Please wait a minute...
浙江大学学报(农业与生命科学版)  2021, Vol. 47 Issue (6): 683-694    DOI: 10.3785/j.issn.1008-9209.2021.06.151
综述     
我国主要园艺植物天然产物研究进展
梁姣娇(),陈云义(),王岳,陈杰标,曹锦萍,李鲜,孙崇德()
浙江大学果实品质生物学实验室/农业农村部园艺植物生长发育与品质调控重点开放实验室/园艺产品冷链物流工艺与装备国家地方联合工程实验室/园艺植物整合生物学研究与应用浙江省重点实验室,杭州 310058
Research progress on natural products of main horticultural plants in China
Jiaojiao LIANG(),Yunyi CHEN(),Yue WANG,Jiebiao CHEN,Jinping CAO,Xian LI,Chongde SUN()
Laboratory of Fruit Quality Biology/Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture and Rural Affairs/Horticultural Products Cold Chain Logistics Technology and Equipment National-Local Joint Engineering Laboratory/Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou 310058, China
 全文: PDF(1438 KB)   HTML
摘要:

天然产物在自然界中广泛存在,其独特的化学多样性赋予它多种与人体健康相关的生物活性。我国园艺植物资源丰富,其中所蕴含的天然产物是值得深入研究与利用的资源宝库。本文主要围绕我国主要园艺植物天然产物的鉴定挖掘、分离纯化及其活性评价应用等方面进行综述,总结归纳了相关研究进展以及不同评价方法的优缺点,旨在为我国主要园艺植物天然产物的深度挖掘利用、活性物质分离效率的提高以及评价技术体系的完善提供参考,从而推进未来我国园艺植物天然产物相关产业融合发展的进程。

关键词: 园艺植物天然产物分离纯化鉴定活性评价    
Abstract:

Natural products are widely found in nature, and their unique chemical diversity endows them with a variety of biological activities related to human health. China is rich in horticultural plant resources, in which the natural products are worthy of in-depth study and utilization. This article mainly focused on the identifi-cation, separation and purification of natural products of main horticultural plants in China and their application in activity evaluation systems, and summarized the progress of related research and the advantages and disadvantages of various evaluation methods, aiming to deepen the utilization of horticultural plant natural products, improve the separation efficiency of active substances and complete the evaluation systems, and further promote the integration and development of horticultural plant natural products related industries in the future.

Key words: horticultural plants    natural products    separation    purification    identification    activity evaluation
收稿日期: 2021-06-15 出版日期: 2021-12-25
CLC:  S 6-0  
基金资助: 国家重点研发计划政府间国际科技创新合作专项(2017YFE0122300);国家自然科学基金(32072132);农业农村部科研杰出人才及其创新团队(果实营养与人类健康创新团队)项目;中央高校基本科研业务费专项资金(2020XZZX003-03)
通讯作者: 孙崇德     E-mail: 12016038@zju.edu.cn;adesun2006@zju.edu.cn
作者简介: 梁姣娇(https://orcid.org/0000-0002-1702-9972),E-mail:12016038@zju.edu.cn|梁姣娇(https://orcid.org/0000-0002-1702-9972),E-mail:12016038@zju.edu.cn
服务  
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章  
梁姣娇
陈云义
王岳
陈杰标
曹锦萍
李鲜
孙崇德

引用本文:

梁姣娇,陈云义,王岳,陈杰标,曹锦萍,李鲜,孙崇德. 我国主要园艺植物天然产物研究进展[J]. 浙江大学学报(农业与生命科学版), 2021, 47(6): 683-694.

Jiaojiao LIANG,Yunyi CHEN,Yue WANG,Jiebiao CHEN,Jinping CAO,Xian LI,Chongde SUN. Research progress on natural products of main horticultural plants in China. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(6): 683-694.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2021.06.151        http://www.zjujournals.com/agr/CN/Y2021/V47/I6/683

图1  有代表性的国家园艺植物种质资源圃在全国各地的分布情况
图2  园艺植物天然产物分离纯化的主要流程与方法
图3  不同活性评价体系的优缺点
  
1 SHEN Y M, HAO X J. Natural product sciences: an integrative approach to the innovations of plant natural products. Science China (Life Sciences), 2020,63(11):1634-1650. DOI:10.1007/s11427-020-1799-y
doi: 10.1007/s11427-020-1799-y
2 PHAM J V, YILMA M A, FELIZ A, et al. A review of the microbial production of bioactive natural products and biologics. Frontiers in Microbiology, 2019,10:1404. DOI:10.3389/fmicb.2019.01404
doi: 10
3 SOROKINA M, STEINBECK C. Review on natural products databases: where to find data in 2020. Journal of Cheminformatics, 2020,12(9):629-661. DOI:10.1186/s13321-020-00424-9
doi: 10.1186/s13321-020-00424-9
4 MARTEL J, OJCIUS D M, KO Y F, et al. Phytochemicals as prebiotics and biological stress inducers. Trends in Biochemical Sciences, 2020,45(6):462-471. DOI:10.1016/j.tibs.2020.02.008
doi: 10.1016/j.tibs.2020.02.008
5 ATANASOV A G, ZOTCHEV S B, DIRSCH V M, et al. Natural products in drug discovery: advances and oppor-tunities. Nature Reviews: Drug Discovery, 2021,20(3):200-216. DOI:10.1038/s41573-020-00114-z
doi: 10.1038/s41573-020-00114-z
6 NEWMAN D J, CRAGG G M. Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. Journal of Natural Products, 2020,83(3):770-803. DOI:10.1021/acs.jnatprod.9b01285
doi: 10.1021/acs.jnatprod.9b01285
7 PATRIDGE E, GAREISS P, KINCH M S, et al. An analysis of FDA-approved drugs: natural products and their derivatives. Drug Discovery Today, 2016,21(2):204-207. DOI:10.1016/j.drudis.2015.01.009
doi: 10.1016/j.drudis.2015.01.009
8 郭瑞霞,李力更,王于方,等.天然药物化学史话:天然产物化学研究的魅力.中草药,2015,46(14):2019-2033. DOI:10.7501/j.issn.0253-2670.2015.14.001
GUO R X, LI L G, WANG Y F, et al. Historical story on natural medicinal chemistry: great charm of study on natural product chemistry. Chinese Traditional and Herbal Drugs, 2015,46(14):2019-2033. (in Chinese with English abstract)
doi: 10.7501/j.issn.0253-2670.2015.14.001
9 UGBOGU E A, ELGHANDOUR M, IKPEAZU V O, et al. The potential impacts of dietary plant natural products on the sustainable mitigation of methane emission from livestock farming. Journal of Cleaner Production, 2019,213:915-925. DOI:10.1016/j.jclepro.2018.12.233
doi: 10.1016/j.jclepro.2018.12.233
10 LIU R H. Health-promoting components of fruits and vegetables in the diet. Advances in Nutrition, 2013,4(3):384S-392S. DOI:10.3945/an.112.003517
doi: 10.3945/an.112.003517
11 武运芳.新疆雪莲植株再生体系的优化及农杆菌介导的遗传转化研究.北京:中央民族大学,2013. DOI:10.5772/22026
WU Y F. Optimization of plant regeneration system and Agrobacterium-mediated genetic transformation of Saussurea involucrata in Xinjiang. Beijing: Minzu University of China, 2013. (in Chinese with English abstract)
doi: 10.5772/22026
12 赵红艳,江丽丽,马淼.濒危药用植物天山雪莲高效植株再生体系的建立.种子,2012,31(4):1-3. DOI:10.16590/j.cnki.1001-4705.2012.04.053
ZHAO H Y, JIANG L L, MA M. In vitro micro-propagation system of Saussurea involucrata an endangered Chinese medicinal herb. Seed, 2012,31(4):1-3. (in Chinese with English abstract)
doi: 10.16590/j.cnki.1001-4705.2012.04.053
13 靳欣欣,田英姿,英犁,等.新疆地区与黄河流域红富士苹果性状与品质分析.现代食品科技,2016,32(7):249-254. DOI:10.13982/j.mfst.1673-9078.2016.7.038
JIN X X, TIAN Y Z, YING L, et al. Fruit characteristics and quality of Red Fuji apples grown in Xinjiang and Yellow River Basin. Modern Food Science and Technology, 2016,32(7):249-254. (in Chinese with English abstract)
doi: 10.13982/j.mfst.1673-9078.2016.7.038
14 严鑫,吴巨友,贡鑫,等.不同产地圆黄梨果实品质差异分析.果树学报,2021. DOI:10.13925/j.cnki.gsxb.20210255
55. DOI:10.13925/j.cnki.gsxb.20210255
doi: 10.13925/j.cnki.gsxb.20210255
14 YAN X, WU J Y, GONG X, et al. Analysis on the difference of fruit quality of Wonhwang pear from different regions. Journal of Fruit Science, 2021. (in Chinese with English abstract)
doi: 10.13925/j.cnki.gsxb.20210255
15 罗广华,王爱国.植物中的多酚物质对超氧物自由基的清除作用.热带亚热带植物学报,1994,2(4):95-99. DOI:10.3969/j.issn.1005-3395.1994.4.010
LUO G H, WANG A G. Scavenging effect of plant polyphenolics on superoxide radicals. Journal of Tropical and Subtropical Botany, 1994,2(4):95-99. (in Chinese)
doi: 10.3969/j.issn.1005-3395.1994.4.010
16 马燕,徐贞贞,邹辉,等.8个品种辣椒籽成分分析与比较.食品科学,2017,38(22):178-183. DOI:10.7506/spkx1002-6630-201722027
MA Y, XU Z Z, ZOU H, et al. Analysis?and?comparison?of?constituents?in?hot?pepper?seeds?of?eight?varieties. Food Science, 2017,38(22):178-183. (in Chinese with English abstract)
doi: 10.7506/spkx1002-6630-201722027
17 韩玉竹,李平兰,何琴,等.辣椒籽抗菌肽提取条件优化及分离纯化.食品科学,2019,40(24):258-264. DOI:10.7506/spkx1002-6630-20181112-123
HAN Y Z, LI P L, HE Q, et al. Optimization of extraction conditions, separation and purification of antifungal peptides from hot pepper seeds. Food Science, 2019,40(24):258-264. (in Chinese with English abstract)
doi: 10.7506/spkx
18 冯鑫,夏宇,陈贵堂,等.生姜皮多糖的分离纯化及其结构组成分析.食品科学,2017,38(6):185-190. DOI:10.7506/spkx1002-6630-201706029
FENG X, XIA Y, CHEN G T, et al. Purification and structural analysis of polysaccharides from ginger peels. Food Science, 2017,38(6):185-190. (in Chinese with English abstract)
doi: 10.7506/spkx
19 王思明,付炎,刘丹,等.天然药物化学史话:“四大光谱”在天然产物结构鉴定中的应用.中草药,2016,47(16):2779-2796. DOI:10.7501/j.issn.0253-2670.2016.16.001
WANG S M, FU Y, LIU D, et al. Historical story on natural medicine chemistry: application of UV, IR, MS, and NMR spectra in structure elucidation of natural products. Chinese Traditional and Herbal Drugs, 2016,47(16):2779-2796. (in Chinese with English abstract)
doi: 10.7501/j.issn.0253-2670.2016.16.001
20 郑振佳.牛蒡活性成分与抗Ⅱ型糖尿病功能研究.山东,泰安:山东农业大学,2018. DOI:10.26420/thrombhaemostres.2021.1058
ZHENG Z J. Study on the active components and anti-type Ⅱ diabetes function of Arctium lappa L. (Burdock). Tai’an, Shandong: Shandong Agricultural University, 2018. (in Chinese with English abstract)
doi: 10.26420/thrombhaemostres.2021.1058
21 XU L S, ZHANG Y J, WANG L Z. Structure characteristics of a water-soluble polysaccharide purified from dragon fruit (Hylocereus undatus) pulp. Carbohydrate Polymers, 2016,146:224-230. DOI:10.1016/j.carbpol.2016.03.060
doi: 10.1016/j.carbpol.2016.03.060
22 王晓天,李兴国,李大龙,等.不同树莓品种中非花色苷酚的鉴定与分析.北方园艺,2021(11):36-43. DOI:10.11937/bfyy.20203753
WANG X T, LI X G, LI D L, et al. Identification and analysis of non-anthocyanin phenolics in various raspberry (Rubus spp.) cultivars. Northern Horticulture, 2021(11):36-43. (in Chinese with English abstract)
doi: 10.11937/bfyy.20203753
23 LU S W, ZHANG Y, ZHU K J, et al. The citrus transcription factor CsMADS6 modulates carotenoid metabolism by directly regulating carotenogenic genes. Plant Physiology, 2018,176(4):2657-2676. DOI:10.1104/pp.17.01830
doi: 10.1104/pp.17.01830
24 陈学森,王楠,张宗营,等.我国高类黄酮(红皮与红肉)苹果育种取得突破性进展.中国果树,2020(2):6-9. DOI:10.16626/j.cnki.issn1000-047.2020.02.002
CHEN X S, WANG N, ZHANG Z Y, et al. Breakthrough in the breeding of high flavonoid (red-skin and red-fleshed) apple in China. China Fruits, 2020(2):6-9. (in Chinese with English abstract)
doi: 10.16626/j.cnki.issn1000-047.2020.02.002
25 吴乾,王艳芳,蔚沐庭,等.红肉苹果果实酚类物质含量及抗氧化性测定.山西农业科学,2020,48(11):1763-1766. DOI:10.3969/j.issn.1002-2481.2020.11.14
WU Q, WANG Y F, YU M T, et al. Determination of phenolics content and antioxidant capacity of red-fleshed apple. Shanxi Agricultural Science, 2020,48(11):1763-1766. (in Chinese with English abstract)
doi: 10.3969/j.issn.1002-2481.2020.11.14
26 TAO L, ZHUO Y T, QIAO Z H, et al. Prenylated coumarins from the fruits of Artocarpus heterophyllus with their potential anti-inflammatory and anti-HIV activities. Natural Product Research, 2021. . DOI:10.1080/14786419.2021.1913590
doi: 10.1080/14786419.2021.1913590
27 陈士林,孙奕,万会花,等.中药与天然药物2015—2020年研究亮点评述.药学学报,2020,55(12):2751-2776. DOI:10.16438/j.0513-4870.2020-1170
CHEN S L, SUN Y, WAN H H, et al. Highlights on the progress of traditional Chinese medicine and natural drugs during 2015—2020. Acta Pharmaceutica Sinica, 2020,55(12):2751-2776. (in Chinese with English abstract)
doi: 10.16438/j.0513-4870.2020-1170
28 ZHANG G J, LI B, CUI H M, et al. Orychophragines A-C, three biologically active alkaloids from Orychophragmus violaceus. Organic Letters, 2018,20:656-659. DOI:10.1021/acs.orglett.7b03801
doi: 10.1021/acs.orglett.7b03801
29 ZHAO Q, GAO J J, QIN X J, et al. Hedychins A and B 6, 7-dinorlabdane diterpenoids with a peroxide bridge from Hedychium forrestii. Organic Letters, 2018,20:704-707. DOI:10.1021/acs.orglett.7b03836
doi: 10.1021/acs.orglett.7b03836
30 黄永娟,张凤启,杨甜甜,等.EMS诱变甘蓝型油菜获得高油酸突变体.分子植物育种,2011,9(5):611-616. DOI:10.3969/mpb.009.000611
HUANG Y J, ZHANG F Q, YANG T T, et al. High oleate mutants of Brassica napus produced by EMS inducement. Molecular Plant Breeding, 2011,9(5):611-616. (in Chinese with English abstract)
doi: 10.3969/mpb.009.000611
31 SONG C, LIU Y F, SONG A P, et al. The Chrysanthemum nankingense genome provides insights into the evolution and diversification of chrysanthemum flowers and medicinal traits. Molecular Plant, 2018,11(12):1482-1491. DOI:10.1016/j.molp.2018.10.003
doi: 10.1016/j.molp.2018.10.003
32 SU W B, JING Y, LIN S K, et al. Polyploidy underlies co-option and diversification of biosynthetic triterpene pathways in the apple tribe. PNAS, 2021,118(20):e2101767118. DOI:10.1073/PNAS.2101767118
doi: 10.1073/PNAS.2101767118
33 LIU M J, ZHAO J, CAI Q H, et al. The complex jujube genome provides insights into fruit tree biology. Nature Communications, 2014,5:5315. DOI:10.1038/ncomms6315
doi: 10.1038/ncomms6315
34 ZHANG Q, WANG L L, LIU Z G, et al. Transcriptome and metabolome profiling unveil the mechanisms of Ziziphus jujuba Mill. peel coloration. Food Chemistry, 2020,312:125903. DOI:10.1016/j.foodchem.2019.125903
doi: 10.1016/j.foodchem.2019.125903
35 LIN T, ZHU G T, ZHANG J H, et al. Genomic analyses provide insights into the history of tomato breeding. Nature Genetics, 2014,46:1220-1226. DOI:10.1038/ng.3117
doi: 10.1038/ng.3117
36 CHEN W, GAO Y Q, XIE W B, et al. Genome-wide association analyses provide genetic and biochemical insights into natural variation in rice metabolism. Nature Genetics, 2014,46:714-721. DOI:10.1038/ng.3007
doi: 10.1038/ng.3007
37 ZHU G T, WANG S C, HUANG Z J, et al. Rewiring of the fruit metabolome in tomato breeding. Cell, 2018,172(1/2):249-261. DOI:10.1016/j.cell.2017.12.019
doi: 10.1016/j.cell.2017.12.019
38 GUO S G, ZHAO S J, SUN H H, et al. Resequencing of 414 cultivated and wild watermelon accessions identifies selection for fruit quality traits. Nature Genetics, 2019,51:1616-1623. DOI:10.1038/s41588-019-0518-4
doi: 10.1038/s41588-019-0518-4
39 ZHANG M Y, XUE C, HU H J, et al. Genome-wide association studies provide insights into the genetic determi-nation of fruit traits of pear. Nature Communications, 2021,12:1144. DOI:10.1038/S41467-021-21378-Y
doi: 10.1038/S41467-021-21378-Y
40 王仁才.果蔬营养与健康.北京:化学工业出版社,2013:184-192.
WANG R C. Nutrition and Health of Fruits and Vegetables. Beijing: Chemical Industry Press, 2013:184-192. (in Chinese)
41 刘静波,陈晶晶,王二雷,等.蓝莓果实中花色苷单体的色谱分离纯化.食品科学,2017,38(2):206-213. DOI:10.7506/spkx1002-6630-201702033
LIU J B, CHEN J J, WANG E L, et al. Separation of anthocyanin monomers from blueberry fruits through chroma-tographic techniques. Food Science, 2017,38(2):206-213. (in Chinese with English abstract)
doi: 10.7506/spkx
42 PENG J M, LI K K, ZHU W, et al. Separation and purification of four phenolic compounds from persimmon by high-speed counter-current chromatography. Journal of Chromatography B, 2018,1072:78-85. DOI:10.1016/j.jchromb.2017.11.010
doi: 10.1016/j.jchromb.2017.11.010
43 MA Q, LUO J G, KONG L Y. Preparative isolation of steroidal saponins from garlic (Allium Sativum L.) using high-speed counter-current chromatography coupled with evaporative light scattering detection. Journal of Liquid Chromatography & Related Technologies, 2011,34(17):1997-2007. DOI:10.1080/10826076.2011.582911
doi: 10.1080/10826076.2011.582911
44 LI Y N, YIN L H, ZHENG L L, et al. Application of high-speed counter-current chromatography coupled with a reverse micelle solvent system to separate three proteins from Momordica charantia. Journal of Chromatography B, 2012,895/896:77-82. DOI:10.1016/j.jchromb.2012.03.017
doi: 10.1016/j.jchromb.2012.03.017
45 芦鑫,洪梦佳,宋国辉,等.树莓汁中黄酮的初级分离纯化.食品科技,2017,42(2):197-203. DOI:10.13684/j.cnki.spkj.2017.02.039
LU X, HONG M J, SONG G H, et al. Preliminary separation and purification of flavone in raspberry juice. Food Science and Technology, 2017,42(2):197-203. (in Chinese with English abstract)
doi: 10.13684/j.cnki.spkj.2017.02.039
46 WANG K B, CHEN Q C, LIN Y, et al. Separation of catechins and O-methylated (-)-epigallocatechin gallate using polyamide thin-layer chromatography. Journal of Chromatography B, 2016,1017/1018:221-225. DOI:10.1016/j.jchromb.2015.11.060
doi: 10.1016/j.jchromb.2015.11.060
47 JIN X, LIU M Y, CHEN Z X, et al. Separation and purification of epigallocatechin-3-gallate (EGCG) from green tea using combined macroporous resin and polyamide column chromatography. Journal of Chromatography B, 2015,1002:113-122. DOI:10.1016/j.jchromb.2015.07.055
doi: 10.1016/j.jchromb.2015.07.055
48 中华人民共和国卫生部.关于进一步规范保健食品原料管理的通知.2002-02-28.[2021-06-12]. . DOI:10.1145/511446.511450
Ministry of Health of the People’s Republic of China. Notice on further standardizing the management of health food raw materials. 2002-02-28.[].
doi: 10.1145/511446.511450
49 高雪婧,罗思奕,汤凯洁,等.山楂粗糖蛋白对高脂小鼠降脂及抗氧化作用的初步研究.食品与发酵工业,2021,47(1):138-142. DOI:10.13995/j.cnki.11-1802/ts.024873
GAO X J, LUO S Y, TANG K J, et al. Effect of crude hawthorn glycoprotein on hypolipidemic and antioxidant activity in high-fat mice. Food and Fermentation Industries, 2021,47(1):138-142. (in Chinese with English abstract)
doi: 10.13995/j.cnki.11-1802/ts.024873
50 李丽静,王领弟,吴晓光.山楂叶总黄酮通过调控miR-133b改善缺氧复氧诱导的神经细胞损伤.中成药,2020,42(6):1443-1449. DOI:10.3969/j.issn.1001-1528.2020.06.009
LI L J, WANG L D, WU X G. Effects of hawthorn leaf flavonoids on improvement of hypoxia-reoxygenation-induced neuronal damage via miR-133b regulation. Chinese Traditional Patent Medicine, 2020,42(6):1443-1449. (in Chinese with English abstract)
doi: 10.3969/j.issn.1001-1528.2020.06.009
51 FU X T, YANG H H, MA C L, et al. Characterization and inhibitory activities on α-amylase and α-glucosidase of the polysaccharide from blue honeysuckle berries. International Journal of Biological Macromolecules, 2020,163:414-422. DOI:10.1016/j.ijbiomac.2020.06.267
doi: 10.1016/j.ijbiomac.2020.06.267
52 PAUL S, ZHANG X M, YANG Y P, et al. Chemical constituents from turnip and their effects on α-glucosidase. Phyton—International Journal of Experimental Botany, 2020,89(1):131-136. DOI:10.32604/phyton.2020.08328
doi: 10.32604/phyton.2020.08328
53 ZHONG Y L, XU H, ZHONG Y, et al. Identification and characterization of the Cucurbitacins, a novel class of small-molecule inhibitors of tropomyosin receptor kinase a. BMC Complementary and Alternative Medicine, 2019,19:295. DOI:10.1186/s12906-019-2709-z
doi: 10.1186/s12906-019-2709-z
54 CHEN F, HUANG G L, HUANG H L. Preparation, analysis, antioxidant activities in vivo of phosphorylated polysaccharide from Momordica charantia. Carbohydrate Polymers, 2021,252:117179. DOI:10.1016/j.carbpol.2020.117179
doi: 10.1016/j.carbpol.2020.117179
55 冯尚坤,陈浩,邵志勇,等.红光处理对萝卜芽菜采后贮藏过程中芥子油苷和抗氧化能力的影响.核农学报,2021,35(6):1340-1346. DOI:10.11869/j.issn.100-8551.2021.06.1340
FENG S K, CHEN H, SHAO Z Y, et al. Effect of red light treatment on glucosinolates and antioxidant capacity in radish sprouts during post-harvest storage. Journal of Nuclear Agricultural Sciences, 2021,35(6):1340-1346. (in Chinese with English abstract)
doi: 10.11869/j.issn.100-8551.2021.06.1340
56 赵彤,安慧,李佳文,等.外源茉莉酸对盐芥芥子油苷含量与抗氧化特性的影响.植物生理学报,2021,57(3):614-622. DOI:10.3969/j.issn.1000-3150.2020.12.001
ZHAO T, AN H, LI J W, et al. Effects of exogenous jasmonic acid on glucosinolate content and antioxidant properties of Eutrema salsugineum. Plant Physiology Journal, 2021,57(3):614-622. (in Chinese with English abstract)
doi: 10.3969/j.issn.1000-3150.2020.12.001
57 LI Y, CHENG X Y, CHEN C L, et al. Apigenin, a flavonoid constituent derived from P. villosa, inhibits hepatocellular carcinoma cell growth by CyclinD1/CDK4 regulation via p38 MAPK-p21 signaling. Pathology—Research and Practice, 2020,216(1):152701. DOI:10.1016/j.prp.2019.152701
doi: 10.1016/j.prp.2019.152701
58 DONG J, ZHANG X, ZHANG L, et al. Quercetin reduces obesity-associated ATM infiltration and inflammation in mice: a mechanism including AMPKα1/SIRT1. Journal of Lipid Research, 2014,55(3):363-374. DOI:10.1194/jlr.M038786
doi: 10.1194/jlr.M038786
59 CHEN J B, WANG Y, ZHU T L, et al. Beneficial regulatory effects of polymethoxyflavone-rich fraction from ougan (Citrus reticulata cv. Suavissima) fruit on gut microbiota and identification of its intestinal metabolites in mice. Antioxidants, 2020,9(9):831. DOI:10.3390/antiox9090831
doi: 10.3390/antiox9090831
60 JIA C S, CAO D D, JI S P, et al. Whey protein isolate conjugated with xylo-oligosaccharides via Maillard reaction: characterization, antioxidant capacity, and application for lycopene microencapsulation. LWT-Food Science and Tech-nology, 2020,118:108837. DOI:10.1016/j.lwt.2019.108837
doi: 10.1016/j.lwt.2019.108837
61 SUN Y, CHI J P, YE X Q, et al. Nanoliposomes as delivery system for anthocyanins: physicochemical characterization, cellular uptake, and antioxidant properties. LWT-Food Science and Technology, 2021,139:110554. DOI: 10.1016/j.lwt.2020.110554
doi: 10.1016/j.lwt.2020.110554
62 屠幼英,马驰.近50年我国茶资源综合利用研究成果及应用进展.中国茶叶,2020,42(12):1-8. DOI:10.3969/j.issn.1000-3150.2020.12.001
TU Y Y, MA C. Research achievements and application progress of utilizations of tea resources in China in recent 50 years. China Tea, 2020,42(12):1-8. (in Chinese with English abstract)
doi: 10.3969/j.issn.1000-3150.2020.12.001
63 朱纪林,郝建宏.涉及蓝莓的化妆品专利技术综述.江西化工,2019(2):190-192. DOI:10.3969/j.issn.1008-3103.2019.02.055
ZHU J L, HAO J H. Summary of cosmetic patent technology involving blueberries. Jiangxi Chemical Industry, 2019(2):190-192. (in Chinese)
doi: 10.3969/j.issn.1008-3103.2019.02.055
64 彭新,牛乐,周宁,等.山药多糖功能活性及新产品开发研究进展.食品研究与开发,2020,41(17):204-209. DOI:10.12161/j.issn.1005-6521.2020.17.033
PENG X, NIU L, ZHOU N, et al. Research progress in functional activity and new product development of Chinese yam polysaccharide. Food Research and Development, 2020,41(17):204-209. (in Chinese with English abstract)
doi: 10.12161/j.issn.1005-6521.2020.17.033
65 孙晓波.总皂苷与总黄酮在制备治疗心血管疾病药中的应用:CN201110188718.4. 2011-11-02. DOI:10.1016/j.chmed.2018.12.003
SUN X B. Application of total saponins and total flavonoids in preparing medicines for treating cardiovascular diseases: CN201110188718.4. 2011-11-02. (in Chinese)
doi: 10.1016/j.chmed.2018.12.003
66 中国农业科学院农产品加工研究所.一种柑橘乳酸菌饮料及其制备方法:CN201710038172.1. 2017-06-20. DOI:10.1055/s-0037-1604374
Chinese Academy of Agricultural Sciences. Institute of Food Science and Technology. A citrus lactobacillus beverage and preparation method thereof: CN201710038172.1. 2017-06-20. (in Chinese)
doi: 10.1055/s-0037-1604374
67 中国农业科学院农产品加工研究所.一种含有多甲氧基黄酮的柑橘风味添加剂及其制备方法:CN201811584224.6. 2019-05-28. DOI:10.1055/s-0039-1688666
Chinese Academy of Agricultural Sciences. Institute of Food Science and Technology. A citrus flavor additive containing polymethoxyflavones and a preparation method thereof: CN201811584224.6. 2019-05-28. (in Chinese)
doi: 10.1055/s-0039-1688666
68 中国农业科学院农产品加工研究所.具有改善肠道菌群结构和缓减动脉粥样硬化功能的柑橘产品及其制备方法与应用:CN202010160680.9. 2020-06-05. DOI:10.37544/0720-5953-2020-05-06
Chinese Academy of Agricultural Sciences. Institute of Food Science and Technology. Citrus product with the function of improving the structure of intestinal flora and reducing atherosclerosis and its preparation method and application: CN202010160680.9. 2020-06-05. (in Chinese)
doi: 10.37544/0720-5953-2020-05-06
[1] 朱泰霖,王慧心,陈杰标,王岳,曹锦萍,李鲜,孙崇德. 不同品种柑橘果实的类黄酮分离纯化及其抗氧化活性研究[J]. 浙江大学学报(农业与生命科学版), 2021, 47(6): 704-718.
[2] 陈国户,王浩,李广,唐小燕,汪承刚,张磊,侯金锋,袁凌云. 白菜PRX基因家族的鉴定与生物信息学分析[J]. 浙江大学学报(农业与生命科学版), 2020, 46(6): 677-686.
[3] 邓艳凤,肖水平,杨绍群,刘新稳,柯兴盛,王涛,杨秀. 长江流域油后棉主要农艺性状的鉴定与优异种质筛选[J]. 浙江大学学报(农业与生命科学版), 2020, 46(5): 551-561.
[4] 陈智雄,谭礼强,张钰玉,胥伟,赵许朋,谢文钢,刘阳,刘燕. 藏茶茶褐素组分特征及其清除1,1-二苯基-2-三硝基苯肼自由基活性的比较[J]. 浙江大学学报(农业与生命科学版), 2020, 46(5): 582-590.
[5] 王术荣, 原渊, 刘洋, 孟俊龙. 华美白鳞伞——中国新记录种[J]. 浙江大学学报(农业与生命科学版), 2020, 46(5): 599-603.
[6] 贡嘎,王一飞,格桑卓玛,索朗斯珠,尼玛央宗,拉巴次仁. 1株藏猪源荚膜血清D型多杀性巴氏杆菌的分离鉴定及其生物学特性[J]. 浙江大学学报(农业与生命科学版), 2020, 46(5): 611-617.
[7] 陈海念,冯蓉,杨胜竹,曹本福,文明江,刘丽,陆引罡. 1株生防菌的鉴定及其发酵条件优化[J]. 浙江大学学报(农业与生命科学版), 2020, 46(2): 177-188.
[8] 王凤丽,裘纪莹,戴美学,陈蕾蕾,赵双枝,辛雪,周庆新. 甜樱桃采后贮藏环境中病原菌的分离鉴定及其致病力研究[J]. 浙江大学学报(农业与生命科学版), 2020, 46(1): 126-134.
[9] 李夏,夏文君,毛鍶超,卢舒婷,莫开昆,廖敏,周继勇,郑肖娟. 血清4型禽腺病毒浙江株的分离鉴定及其全基因组序列分析[J]. 浙江大学学报(农业与生命科学版), 2019, 45(5): 635-646.
[10] 杨芳芳,李佳慧,张赛南,王珍,廖志银,王首锋. 重组人乙酰胆碱酯酶基因在毕赤酵母中分泌表达及其对农药的敏感性评估[J]. 浙江大学学报(农业与生命科学版), 2019, 45(3): 317-324.
[11] 何亚涛,高丹丹,甘森宁,孙婷,蔡葵蒸,刘俊林. 怀地黄内生产红色素真菌血红红曲霉(Monascus sanguineus)的分离与鉴定[J]. 浙江大学学报(农业与生命科学版), 2019, 45(1): 1-7.
[12] 吕靖雯, 刘蕊, 李国华, 李红叶, 王洪凯. 柚果面枝孢菌斑点病病原鉴定[J]. 浙江大学学报(农业与生命科学版), 2018, 44(6): 687-694.
[13] 庞钰洁, 李海燕, 竺啸恒, 高福明, 殷益明, 贾惠娟. “三本提”葡萄芽变“11-06-25”的遗传鉴定[J]. 浙江大学学报(农业与生命科学版), 2017, 43(1): 73-80.
[14] 朱红雪, 田忠玲, 蔡瑞杭, 李晓琳, 郑经武. 朝鲜孢囊线虫--浙江省孢囊线虫新记录种[J]. 浙江大学学报(农业与生命科学版), 2017, 43(1): 81-88.
[15] 王丽丽, 国巍, 付春娜, 燕红. 可降解苯酚的产电芽孢杆菌WL027的分离筛选及其产电机制初探[J]. 浙江大学学报(农业与生命科学版), 2016, 42(6): 654-664.