甲壳动物过氧化物还原酶基因的研究进展

doi:10.3785/j.issn.1008-9209.2020.08.051

浙江大学学报（农业与生命科学版）

2021, Vol. 47

Issue (3): 284-294 DOI: 10.3785/j.issn.1008-9209.2020.08.051

综述

甲壳动物过氧化物还原酶基因的研究进展

李辉^1,²(

),冯伟^2,³,于俊杰^2,³,张明胤^2,³,周春妙^2,³,唐永凯^1,^2,³(

)

^1.水产科学国家级实验教学示范中心（上海海洋大学），上海 201306
^2.中国水产科学研究院淡水渔业研究中心，农业农村部淡水渔业与种质资源利用重点实验室，江苏无锡 214081
^3.南京农业大学无锡渔业学院，江苏无锡 214128

Research progress of peroxiredoxingene in crustaceans

Hui LI^1,²(

),Wei FENG^2,³,Junjie YU^2,³,Mingyin ZHANG^2,³,Chunmiao ZHOU^2,³,Yongkai TANG^1,^2,³(

)

^1.National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai 201306, China
^2.Ministry of Agriculture and Rural Affairs Key Laboratory of Freshwater Aquatic Germplasm Resources, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China
^3.Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214128, Jiangsu, China

全文: PDF(952 KB) HTML

摘要：

过氧化物还原酶（peroxiredoxin, Prx）属于抗氧化蛋白超家族，广泛存在于真核生物和原核生物中，在先天免疫中发挥着重要作用。目前，有关甲壳动物Prx基因及其在先天免疫中的作用研究较少且不够深入，仅克隆出部分甲壳动物Prx基因的部分亚型，在生物学功能方面仅仅研究了其抗氧化作用。本文综述了近年来甲壳动物Prx基因的克隆、系统进化、组织表达以及生物学功能，对Prx基因在甲壳动物中的研究前景作出展望，以期为甲壳动物Prx基因结构和生物学功能研究提供参考。

关键词： 甲壳动物; 过氧化物还原酶; 基因克隆; 组织表达; 生物学功能

Abstract:

Peroxiredoxin (Prx) belongs to the superfamily of antioxidant proteins, which widely exists in eukaryotes and prokaryotes, and plays an important role in innate immunity. At present, there are a few researches on the Prx genes in crustaceans, and only partial subtypes of the Prx genes are cloned, and the antioxidant effect is studied only in terms of biological function. In this study, the cloning, phylogeny, tissue expression and biological functions of crustacean Prx genes in recent years were reviewed. Furthermore, researches on Prx genes in crustaceans were prospected, which would provide references for the researches on the structures and biological functions of Prx genes in crustaceans.

Key words: crustacean peroxiredoxin gene cloning tissue expression biological function

收稿日期: 2020-08-05 出版日期: 2021-06-25

CLC:

S 917.4

基金资助: 江苏省农业重大新品种创制项目(PZCZ201749);江苏省现代农业产业技术体系(JATS〔2019〕385);江苏省渔业科技类项目(D2018-4)

通讯作者: 唐永凯 E-mail: 1147067174@qq.com;tangyk@ffrc.cn

作者简介: 李辉（https://orcid.org/0000-0003-2207-9090），E-mail：1147067174@qq.com

	服务
	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	李辉
	冯伟
	于俊杰
	张明胤
	周春妙
	唐永凯

引用本文:

李辉,冯伟,于俊杰,张明胤,周春妙,唐永凯. 甲壳动物过氧化物还原酶基因的研究进展[J]. 浙江大学学报（农业与生命科学版）, 2021, 47(3): 284-294.

Hui LI,Wei FENG,Junjie YU,Mingyin ZHANG,Chunmiao ZHOU,Yongkai TANG. Research progress of peroxiredoxingene in crustaceans. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(3): 284-294.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2020.08.051 或 http://www.zjujournals.com/agr/CN/Y2021/V47/I3/284

表1 基于序列同源性和结构相似性的Prx分类

表2 甲壳动物Prx基因及其编码的氨基酸序列

物种 Species	Prx亚型 Prx subtype	亚细胞定位 Subcellular localization
脊尾白虾 Exopalaemon carinicauda	Prx5	过氧化物酶体（55.6%）、细胞核（22.2%）、细胞质（11.1%）、线粒体（11.1%）
中国对虾 Fenneropenaeus chinensis	Prx	细胞质（60.9%）、细胞核（21.7%）、线粒体（13.0%）、分泌系统囊泡（4.4%）
日本沼虾 Macrobrachium nipponense	Prx	细胞质（47.8%）、线粒体（26.1%）、细胞核（21.7%）、分泌系统囊泡（4.4%）
三疣梭子蟹 Portunus trituberculatus	Prx	细胞质（52.4%）、线粒体（21.7%）、细胞核（13.0%）、过氧化物酶体（4.3%）、内质网（4.3%）、质膜（4.3%）
斑节对虾 Penaeus monodon	Prx1	细胞质（47.9%）、细胞核（30.4%）、线粒体（21.7%）
	Prx4	线粒体（39.2%）、细胞质（17.4%）、内质网（17.4%）、细胞核（13.0%）、空泡（8.7%）、细胞外（4.3%）
	Prx5	线粒体（73.9%）、细胞核（17.4%）、细胞质（8.7%）
	Prxn	细胞质（47.8%）、细胞核（30.4%）、线粒体（17.4%）、高尔基体（4.4%）
中华绒螯蟹 Eriocheir sinensis	Prx	细胞质（52.3%）、线粒体（26.1%）、细胞核（13.0%）、细胞外（4.3%）、空泡（4.3%）
	Prx3	线粒体（91.4%）、细胞质（4.3%）、细胞核（4.3%）
	Prx4	线粒体（43.8%）、细胞外（13.0%）、内质网（13.0%）、细胞质（13.0%）、过氧化物酶体（4.3%）、高尔基体（4.3%）、空泡（4.3%）、细胞核（4.3%）
	Prx5	过氧化物酶体（77.8%）、线粒体（11.1%）、细胞质（11.1%）
	Prx6	细胞质（61.3%）、细胞核（30.4%）、线粒体（4.3%）、高尔基体（4%）
日本对虾 Penaeus japonicus	Prx	细胞质（47.8%）、线粒体（26.1%）、细胞核（21.7%）、分泌系统囊泡（4.4%）
	Prx3	线粒体（82.6%）、细胞核（8.7%）、细胞质（8.7%）
	Prx4	线粒体（34.8%）、细胞质（30.6%）、细胞核（8.7%）、高尔基体（8.7%）、细胞外（4.3%）、内质网（4.3%）、分泌系统囊泡（4.3%）、空泡（4.3%）
	Prx6	细胞质（56.6%）、细胞核（34.8%）、线粒体（4.3%）、高尔基体（4.3%）
印度明对虾 Fenneropenaeus indicus	Prx	细胞质（47.9%）、细胞核（30.4%）、线粒体（21.7%）
平背泥蟹 Eurypanopeus depressus	Prx	细胞质（47.8%）、线粒体（26.3%）、细胞核（13.0%）、空泡（4.3%）、内质网（4.3%）、细胞外（4.3%）
南美白对虾 Penaeus vannamei	Prx	细胞质（43.5%）、细胞核（30.4%）、线粒体（26.1%）
拟穴青蟹 Scylla paramamosain	Prx1/2	细胞质（52.2%）、细胞核（17.4%）、线粒体（17.4%）、过氧化物酶体（8.7%）、内质网（4.3%）
	Prx3	线粒体（87.0%）、细胞质（8.7%）、细胞核（4.3%）
	Prx4	线粒体（34.8%）、内质网（17.5%）、细胞质（13.0%）、细胞核（13.0%）、细胞外（8.7%）、高尔基体（8.7%）、空泡（4.3%）
	Prx5-1	过氧化物酶体（77.8%）、线粒体（11.1%）、细胞质（11.1%）
	Prx5-2	过氧化物酶体（77.8%）、线粒体（11.1%）、细胞质（11.1%）
	Prx6	细胞质（60.9%）、细胞核（30.5%）、高尔基体（4.3%）、线粒体（4.3%）
中华卤虫 Artemia sinica	Prx	细胞质（43.6%）、线粒体（30.4%）、细胞核（21.7%）、分泌系统囊泡（4.3%）
美国大盐湖卤虫 Artemia franciscana	Prx	细胞质（43.5%）、细胞核（30.4%）、线粒体（26.1%）

表3 亚细胞定位分析

图1 基于9种动物Prx基因蛋白序列构建的系统进化树

表4 甲壳动物Prx的生物学功能研究汇总

1	朱江艳.高温胁迫下刺参消化道菌群变化及Prxs基因表达分析.福建,厦门:集美大学,2013. ZHU J Y. Variation of microflora in the digestive tract and Prxs gene expression analysis of Apostichopus japonicas under high-temperature stress. Xiamen, Fujian: Jimei University, 2013. (in Chinese with English abstract)
2	DREVET J R. The antioxidant glutathione peroxidase family and spermatozoa: a complex story. Molecular and Cellular Endocrinology, 2006,250(1):70-79. DOI:10.1016/j.mce.2005.12.027 doi: 10.1016/j.mce.2005.12.027
3	SANLIOGLU S, WILLIAMS C M, SAMAVATI L, et al. Lipopolysaccharide induces Racl-dependent reactive oxygen species formation and coordinates tumor necrosis factor-α secretion through IKK regulation of NF-κB. The Journal of Biological Chemistry, 2001,276(32):30188-30198. DOI:10.1074/jbc.M102061200 doi: 10
4	BOGDAN C, R?LLINGHOFF M, DIEFENBACH A. Reactive oxygen and reactive nitrogen intermediates in innate and specific immunity. Current Opinion in Immunology, 2000,12(1):64-76. DOI:10.1016/s0952-7915(99)00052-7 doi: 10.1016/s0952-7915(99)00052-7
5	ERMAK G, DAVIES K J A. Calcium and oxidative stress: from cell signaling to cell death. Molecular Immunology, 2002,38(10):713-721. DOI:10.1016/S0161-5890(01)00108-0 doi: 10.1016/S0161-5890(01)00108-0
6	PI J B, ZHANG Q, FU J Q, et al. ROS signaling, oxidative stress and Nrf2 in pancreatic beta-cell function. Toxicology and Applied Pharmacology, 2010,244(1):77-83. DOI:10.1016/j.taap.2009.05.025 doi: 10.1016/j.taap.2009.05.025
7	DUMONT M, BEAL M F. Neuroprotective strategies involving ROS in Alzheimer disease. Free Radical Biology and Medicine, 2011,51(5):1014-1026. DOI:10.1016/j.freerad biomed.2010.11.026 doi: 10.1016/j.freerad
8	张星莹,李甜甜,杨春娜,等.过氧化物还原酶在生殖与妊娠并发症中作用机制的研究进展.山东医药,2019,59(1):94-97. DOI:10.3969/j.issn.1002-266X.2019.01.028 ZHANG X Y, LI T T, YANG C N, et al. Research progress on the mechanism of peroxide reductase in reproductive and pregnancy complications. Shandong Medical Journal, 2019,59(1):94-97. (in Chinese with English abstract) doi: 10.3969/j.issn.1002-266X.2019.01.028
9	NATHAN C, SHILOH M U. Reactive oxygen and nitrogen intermediates in the relationship between mammalian hosts and microbial pathogens. PNAS, 2000,97(16):8841-8848. DOI:10.1073/pnas.97.16.8841 doi: 10.1073/pnas.97.16.8841
10	AISPURO-HERNANDEZ E, GARCIA-OROZCO K D, MUHLIA-ALMAZAN A, et al. Shrimp thioredoxin is a potent antioxidant protein. Comparative Biochemistry and Physiology: Part C, 2008,148(1):94-99. DOI:10.1016/j.cbpc.2008.03.013 doi: 10.1016/j.cbpc.2008.03.013
11	NAKAMURA H, NAKAMURA K, YODOI J. Redox regulation of cellular activation. Annual Review of Immunology, 1997,15:351-369.
12	KANG S W, RHEE S G, CHANG T S, et al. 2-Cys peroxiredoxin function in intracellular signal transduction: therapeutic implications. Trends in Molecular Medicine, 2005,11(12):571-578. DOI:10.1016/j.molmed.2005.10.006 doi: 10.1016/j.molmed.2005.10.006
13	章波,向渝梅,白云.抗氧化蛋白Peroxiredoxin家族研究进展.生理科学进展,2004,35(4):352-355. ZHANG B, XIANG Y M, BAI Y. Research progress of antioxidant protein peroxiredoxin family. Advances in Physiological Science, 2004,35(4):352-355. (in Chinese)
14	张海燕,李国田,王晓杰,等.小麦过氧化物还原酶基因TaPrx的克隆与功能初步分析.中国农业科学,2009,42(4):1222-1229. DOI:10.3864/j.issn.0578-1752.2009.04.013 ZHANG H Y, LI G T, WANG X J, et al. Cloning and primary characteration analysis of peroxiredoxin gene (TaPrx) from wheat. Scientia Agricultura Sinica, 2009,42(4):1222-1229. (in Chinese with English abstract) doi: 10.3864/j.issn.0578-1752.2009.04.013
15	岳思君,周娟,郑蕊,等.发状念珠藻过氧化物还原酶NfPrx基因的克隆与表达分析.植物生理学报,2016,52(8):1287-1294. DOI:10.13592/j.cnki.ppj.2016.0241 YUE S J, ZHOU J, ZHENG R, et al. Cloning and expression analysis of NfPrx gene from Nostoc flagelliforme. Plant Physiology Journal, 2016,52(8):1287-1294. (in Chinese with English abstract) doi: 10.13592/j.cnki.ppj.2016.0241
16	孙晶.七鳃鳗过氧化物还原酶2(Peroxiredoxin 2)基因克隆、表达及生物学活性研究.辽宁,大连:辽宁师范大学,2010. SUN J. Gene cloning, expression and biological activity of a peroxiredoxin 2 homologue from Lampetra japonica. Dalian, Liaoning: Liaoning Normal University, 2010. (in Chinese with English abstract)
17	陈晓峰,胡盼,李岩松,等.小鼠Peroxiredoxin 6基因克隆、原核表达及多克隆抗体的制备.中国生物工程杂志,2016,36(3):11-16. DOI:10.13523/j.cb.20160302 CHEN X F, HU P, LI Y S, et al. Molecular cloning, prokaryotic expression and polyclonal antibody preparation of Peroxiredoxin 6 (Prdx6) from Mus musculus. China Biotechnology, 2016,36(3):11-16. (in Chinese with English abstract) doi: 10.13523/j.cb.20160302
18	裴鹏祖.褶纹冠蚌1-Cys和2-Cys型过氧化物还原酶基因的分子克隆及表达.南昌:南昌大学,2011. PEI P Z. Molecular cloning and expression of 1-Cys and 2-Cys peroxiredoxins from Cristaria plicata. Nanchang: Nanchang University, 2011. (in Chinese with English abstract)
19	RADYUK S N, KLICHKO V I, SPINOLA B, et al. The peroxiredoxin gene family in Drosophila melanogaster. Free Radical Biology and Medicine, 2001,31(9):1090-1100. DOI:10.1016/S0891-5849(01)00692-X doi: 10.1016/S0891-5849(01)00692-X
20	LEYENS G, DONNAY I, KNOOPS B. Cloning of bovine peroxiredoxins-gene expression sequence comparison with rat, in bovine tissues and amino acid mouse and primate peroxiredoxins. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2003,136(4):943-955. DOI:10.1016/S1096-4959(03)00290-2 doi: 10.1016/S1096-4959(03)00290-2
21	RHEE S G, CHAE H Z, KIM K. Peroxiredoxins: a historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling. Free Radical Biology and Medicine, 2005,38(12):1543-1552. DOI:10.1016/j.freerad biomed.2005.02.026 doi: 10.1016/j.freerad
22	CHEN J W, DODIA C, FEINSTEIN S I, et al. 1-Cys peroxiredoxin, a bifunctional enzyme with glutathione peroxidase and phospholipase A2 activities. The Journal of Biological Chemistry, 2000,275(37):28421-28427. DOI:10.1074/jbc.M005073200 doi: 10
23	DUAN Y F, LIU P, LI J T, et aI. cDNA cloning, characterization and expression analysis of peroxiredoxin 5 gene in the ridgetail white prawn Exopalaemon carinicauda. Molecular Biology Reports, 2013,40(12):6569-6577. DOI:10.1007/s11033-013-2702-4 doi: 10
24	KINNULA V L, LEHTONEN S, SORMUNEN R, et al. Overexpression of peroxiredoxinsⅠ, Ⅱ, Ⅲ, Ⅴ, and Ⅵ in malignant mesothelioma. The Journal of Pathology, 2002,196(3):316-323. DOI:10.1002/path.1042 doi: 10.1002/path.1042
25	OBERLEY T D, VERWIEBE E, ZHONG W X, et al. Localization of the thioredoxin system in normal rat kidney. Free Radical Biology and Medicine, 2001,30(4):412-424. DOI:10.1016/S0891-5849(00)00486-X doi: 10.1016/S0891-5849(00)00486-X
26	WATABE S, HIROI T, YAMAMOTO Y, et al. SP-22 is a thioredoxin-dependent peroxide reductase in mitochondria. FEBS Journal, 1997,249(1):52-60. DOI:10.1111/j.1432-1033.1997.t01-1-00052.x doi: 10.1111/j.1432-1033
27	KNOOPS B, CLIPPE A, BOGARD C, et al. Cloning and characterization of AOEB166, a novel mammalian antioxidant enzyme of the peroxiredoxin family. Journal of Biological Chemistry, 1999,274(43):30451-30458. DOI:10.1074/jbc.274.43.30451 doi: 10
28	PéREZ-SáNCHEZ J, BERMEJO-NOGALES A, CALDUCH-GINER J A, et al. Molecular characterization and expression analysis of six peroxiredoxin paralogous genes in gilthead sea bream (Sparus aurata): insights from fish exposed to dietary, pathogen and confinement stressors. Fish & Shellfish Immunology, 2011,31(2):294-302. DOI:10.1016/j.fsi.2011.05.015 doi: 10.1016/j.fsi.2011.05.015
29	RHEE S G, WOO H A. Multiple functions of peroxiredoxins: peroxidases, sensors regulators of the intracellular messenger H2O2, and protein chaperones. Antioxidants & Redox Signaling, 2011,15(3):781-794. DOI:10.1089/ars.2010.3393 doi: 10.1089/ars.2010.3393
30	SEO M S, KANG S W, KIM K, et al. Identification of a new type of mammalian peroxiredoxin that forms an intra-molecular disulfide as a reaction intermediate. Journal of Biological Chemistry, 2000,275(27):20346-20354. DOI:10.1074/jbc.M001943200 doi: 10
31	GEORGIOU G, MASIP L. An overoxidation journey with a return ticket. Science, 2003,300(5619):592-594. DOI:10.1126/science.1084976 doi: 10.1126/science.1084976
32	SIMONI S D, LINARD D, HERMANS E, et al. Mitochondrial peroxiredoxin-5 as potential modulator of mitochondria-ER crosstalk in MPP+-induced cell death. Journal of Neurochemistry, 2013,125(3):473-485. DOI:10.1111/jnc.12117 doi: 10.1111/jnc.12117
33	MANEVICH Y, FISHER A B. Peroxiredoxin 6, a 1-Cys peroxiredoxin, functions in antioxidant defense and lung phospholipid metabolism. Free Radical Biology and Medicine, 2005,38(11):1422-1432. DOI:10.1016/j.freeradbiomed.2005.02.011 doi: 10.1016/j.freeradbiomed.2005.02.011
34	TU D D, ZHOU Y L, GU W B, et al. Identification and characterization of six peroxiredoxin transcripts from mud crab Scylla paramamosain: the first evidence of peroxiredoxin gene family in crustacean and their expression profiles under biotic and abiotic stresses. Molecular Immunology, 2018,93:223-235. DOI:10.1016/j.molimm.2017.11.029 doi: 10.1016/j.molimm.2017.11.029
35	卜瑞倩.斑节对虾三种过氧化物还原酶在应激条件下的表达与功能分析.上海:上海海洋大学,2018. BU R Q. Three peroxiredoxins from Penaeus monodon: identification, characterization, functional analysis under biotic and abiotic stresses. Shanghai: Shanghai Ocean University, 2018. (in Chinese with English abstract)
36	R?SZER T. The invertebrate midintestinal gland (“hepatopancreas”) is an evolutionary forerunner in the integration of immunity and metabolism. Cell and Tissue Research, 2014,358(3):685-695. DOI:10.1007/s00441-014-1985-7 doi: 10.1007/s00441-014-1985-7
37	GROSS P S, BARTLETT T C, BROWDY C L, et al. Immune gene discovery by expressed sequence tag analysis of hemocytes and hepatopancreas in the Pacific White Shrimp, Litopenaeus vannamei, and the Atlantic White Shrimp, L. setiferus. Developmental & Comparative Immunology, 2001,25(7):565-577. DOI:10.1016/S0145-305X(01)00018-0 doi: 10.1016/S0145-305X(01)00018-0
38	ZHANG Q L, HUANG J, LI F H, et al. Molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin4 in Fen-neropenaeus chinensis and its antioxidant activity. Fish & Shellfish Immunology, 2014,37(1):38-45. DOI:10.1016/j.fsi.2013.12.026 doi: 10.1016/j.fsi.2013.12.026
39	ZHANG Q L, LI F H, ZHANG J Q, et al. Molecular cloning, expression of a peroxiredoxin gene in Chinese shrimp Fenneropenaeus chinensis and the antioxidant activity of its recombinant protein. Molecular Immunology, 2007,44(14):3501-3509. DOI:10.1016/j.molimm.2007.03.014 doi: 10.1016/j.molimm.2007.03.014
40	税典章.中华绒螯蟹(Eriocheir sinensis)2-Cys过氧化物还原酶的cDNA克隆及表达模式研究.江苏,苏州:苏州大学,2019. SHUI D Z. cDNA cloning and expression patterns of 2-Cys Prxs in Eriocheir Sinensis. Suzhou, Jiangsu: Suzhou University, 2019. (in Chinese with English abstract)
41	母昌考.中华绒螯蟹(Eriocheir Sinensis)Peroxiredoxin 6和Thioredoxin 1基因的克隆及表达.山东,青岛:中国科学院海洋研究所,2009. MU C K. Molecular cloning and recombinant expression of Peroxiredoxins 6 and Thioredoxin 1 from Chinese mitten crab Eriocheir Sinensis. Qingdao, Shandong: Institute of Oceanography, Chinese Academy of Sciences,2009. (in Chinese with English abstract)
42	RHEE S G, KANG S W, JEONG W, et al. Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins. Current Opinion in Cell Biology, 2005,17(2):183-189. DOI:10.1016/j.ceb.2005.02.004 doi: 10.1016/j.ceb.2005.02.004
43	WOOD Z A, SCHRODER E, HARRIS J R, et al. Structure, mechanism and regulation of peroxiredoxins. Trends in Biochemical Sciences, 2003,28(1):32-40. DOI:10.1016/S0968-0004(02)00003-8 doi: 10.1016/S0968-0004(02)00003-8
44	RHEE S G, BAE Y S, LEE S R, et al. Hydrogen peroxide: a key messenger that modulates protein phosphorylation through cysteine oxidation. Science’s Stke: Signal Transduction Knowledge Environment, 2000,2000(53):PE1. DOI:10.1126/stke.2000.53.pe1 doi: 10.1126/stke.2000.53.pe1
45	FUJII J, IKEDA Y. Advances in our understanding of peroxiredoxin, a multifunctional, mammalian redox protein. Redox Report, 2002,7(3):123-130. DOI:10.1179/135100002125000352 doi: 10.1179/135100002125000352
46	RHEE S G, CHANG T S, BAE Y S, et al. Cellular regulation by hydrogen peroxide. Journal of the American Society of Nephrology, 2003,14():S211-S215. DOI:10.1097/01.ASN.0000077404.45564.7E doi: 10.1097/01.ASN.0000077404.45564.7E
47	THANNICKAL V J, FANBURG B L. Reactive oxygen species in cell signaling. American Journal of Physiology: Lung Cellular and Molecular Physiology, 2000,279(6):L1005-L1028. DOI:10.1152/ajplung.2000.279.6.L1005 doi: 10.1152/ajplung.2000.279.6.L1005
48	谢亚凯.两种过氧化物还原酶在日本囊对虾先天免疫中的功能研究.济南:山东大学,2016. XIE Y K. The function analysis of two peroxiredoxin subfamilies in innate immunity of Kuruma shrimp Marsupenaeus japonicas. Jinan: Shandong University, 2016. (in Chinese with English abstract)
49	CHEN P, LI J T, GAO B Q, et al. cDNA cloning and characterization of peroxiredoxin gene from the swimming crab Portunus trituberculatus. Aquaculture, 2011,322/323:10-15. DOI:10.1016/j.aquaculture.2011.09.009 doi: 10.1016/j.aquaculture.2011.09.009
50	郭宁宁.过氧化物还原酶(Peroxiredoxin)4在日本囊对虾抗WSSV病毒感染中的功能与机制研究.济南:山东大学,2019. GUO N N. The mechanism study on the ant-virus function of Peroxiredoxin4 (Prx4) in Kuruma shrimp Marsupenaeus japonicas. Jinan: Shandong University, 2019. (in Chinese with English abstract)
51	周茜.中华卤虫滞育卵发育和重金属刺激相关蛋白质组学研究.山东,青岛:中国科学院海洋研究所,2008. ZHOU Q. The proteomic analysis of diapause embryo development and heavy metal stress response in Artemia sinica. Qingdao, Shandong: Institute of Oceanography, Chinese Academy of Sciences, 2008. (in Chinese with English abstract)

[1]	刘慧春,张加强,马广莹,周江华,许雯婷,朱开元. *牡丹PsDHN1基因克隆及转基因拟南芥的耐涝性分析*[J]. 浙江大学学报（农业与生命科学版）, 2021, 47(3): 335-346.
[2]	王鹏杰,岳川,陈笛,郑玉成,郑知临,林浥,杨江帆,叶乃兴. *茶树CsWRKY6、CsWRKY31和CsWRKY48基因的分离及表达分析*[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(1): 30-38.
[3]	张驰, 刘丹丹, 刘建中. 植物J蛋白的生物学功能及其作用机制[J]. 浙江大学学报（农业与生命科学版）, 2018, 44(3): 275-282.
[4]	许抗抗，丁天波，严毅，李灿，杨文佳. CO₂气调胁迫下烟草甲谷胱甘肽S-转移酶基因的表达分析[J]. 浙江大学学报(农业与生命科学版), 2017, 43(5): 599-607.
[5]	郑群艳，潘晓艺，沈锦玉，陈少波，徐洋，许婷. 罗氏沼虾谷氨酸脱氢酶基因克隆及其在MrTV 感染下的组织表达分析[J]. 浙江大学学报(农业与生命科学版), 2017, 43(5): 639-648.
[6]	王家庆，董慧明，李振刚，李绍明，王若楠，付玉洁. 青鳉组织蛋白酶E基因全长cDNA克隆与功能预测[J]. 浙江大学学报(农业与生命科学版), 2017, 43(2): 183-191.
[7]	关小燕，陈丽妃，何艳军，王洁，卢钢. 番茄SlMAPK7基因的亚细胞定位与组织表达特性*[J]. 浙江大学学报(农业与生命科学版), 2014, 40(6): 598-604.
[8]	葛延松，曹嫦妤，王丽丽，李楠，江秀清，李金龙. 鸡硒蛋白T的硒代半胱氨酸插入序列元件、蛋白结构与功能及组织表达差异*[J]. 浙江大学学报(农业与生命科学版), 2014, 40(1): 9-15.
[9]	卢泳全1，贾庆1，童再康1，陈见阳2. 柳杉属3个查尔酮合成酶（CHS）新基因的克隆及其序列特异性分析(英文)*[J]. 浙江大学学报(农业与生命科学版), 2013, 39(3): 246-252.
[10]	蒋明,陈孝赏,李金枝. 紫菜薹花青素合成酶基因BcANS的克隆、表达与序列分析[J]. 浙江大学学报(农业与生命科学版), 2011, 37(4): 393-398.
[11]	宋晓毅, 徐幼平, 张志新, 徐秋芳, 蔡新忠. 8个番茄ACE全长cDNA序列的克隆和分析[J]. 浙江大学学报(农业与生命科学版), 2009, 35(3): 249-254.
[12]	俞路,王雅倩,章世元,李燕舞,李治学. *鸡CaBP-D28k基因重组质粒构建*[J]. 浙江大学学报(农业与生命科学版), 2008, 34(6): 608-614.
[13]	石生林　潘敏慧　鲁成. 柞蚕核型多角体病毒ptp-2基因克隆及表达[J]. 浙江大学学报(农业与生命科学版), 2007, 33(2): 125-128.
[14]	李卫芬　陆平　周绪霞. 多粘芽孢杆菌β-葡聚糖酶特性及其基因克隆[J]. 浙江大学学报(农业与生命科学版), 2004, 30(3): 331-335.
[15]	汪以真　刘光富　初晓娜　韩菲菲. 抗菌肽Protegrin-1基因的PCR扩增和克隆及序列分析[J]. 浙江大学学报(农业与生命科学版), 2004, 30(3): 336-339.

Viewed

Full text

Abstract

Cited

Shared

Discussed