芥蓝Aux/IAA家族基因生物信息学与表达分析

doi:10.3785/j.issn.1008-9209.2020.10.221

浙江大学学报（农业与生命科学版）

2021, Vol. 47

Issue (3): 314-324 DOI: 10.3785/j.issn.1008-9209.2020.10.221

园艺学

芥蓝Aux/IAA家族基因生物信息学与表达分析

王尤轩¹(

),王梦雨¹,李煜博¹,陶晗¹,夏楚楚¹,黄凯美²(

),汪俏梅¹(

)

^1.浙江大学农业与生物技术学院园艺系/农业农村部园艺植物生长发育与品质控制重点开放实验室，杭州 310058
^2.杭州市农业技术推广中心，杭州 310017

Bioinformatics and expression analysis of Aux/IAA family gene in Chinese kale

Youxuan WANG¹(

),Mengyu WANG¹,Yubo LI¹,Han TAO¹,Chuchu XIA¹,Kaimei HUANG²(

),Qiaomei WANG¹(

)

^1.Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University/Ministry of Agriculture and Rural Affairs Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Hangzhou 310058, China
^2.Hangzhou Agricultural Technology Extension Center, Hangzhou 310017, China

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摘要：

为更好地理解芥蓝生长素（indole-3-acetic acid, IAA）的响应途径及揭示Aux/IAA基因家族在芥蓝生长和抗盐胁迫中的功能，本研究利用生物信息学的方法从芥蓝基因组中鉴定到了41个Aux/IAA家族基因。系统进化分析结果表明，芥蓝Aux/IAA基因家族成员分布于9条染色体上，在染色体C01和C05上分布的基因最多，各有6个；该家族基因编码的蛋白质的氨基酸残基数在128～427之间，大多数为亲水性蛋白，仅有2个疏水性蛋白。亚细胞定位预测结果表明，Aux/IAA家族成员多位于细胞质、细胞核和叶绿体上。实时荧光定量聚合酶链式反应（real-time quantitative polymerase chain reaction, qRT-PCR）结果表明，芥蓝Aux/IAA基因家族部分成员对生长素（IAA）、脱落酸（abscisic acid, ABA）、油菜素内酯（brassinosteroids, BR）处理存在不同程度的响应，说明芥蓝Aux/IAA基因家族很可能参与植物激素信号的传导。此外，经过盐胁迫处理后，芥蓝BoIAA19-1与BoIAA2-1的表达量在处理前期均显著上调，预示Aux/IAA基因在芥蓝响应逆境的过程中也可能发挥作用。本研究结果为进一步研究Aux/IAA基因家族在芥蓝生长和抗性平衡中的功能奠定了理论基础。

关键词： 芥蓝; Aux/IAA基因家族; 生物信息学分析; 表达分析

Abstract:

To better understand the indole-3-acetic acid (IAA) response pathway and explore thespecific functions of Aux/IAA gene family in the growth of Chinese kale and in response to salt stress, forty-one Aux/IAA genes were collected and analyzed by bioinformatics methods from Chinese kale genome. Phylogenetic analysis showed that the Aux/IAA genes of Chinese kale were distributed on nine chromosomes and the most genes were distributed on chromosome C01 and C05 including six members, respectively. The number of the amino acid residues of proteins encoded by Aux/IAA genes ranged from 128 to 427, and most of them were hydrophilic proteins, and only two were hydrophobic proteins. The subcellular localization analysis indicated that the Aux/IAA family members were mostly located in cytoplasm, nucleus and chloroplast. The results of real-time quantitative polymerase chain reaction (qRT-PCR) showed that several Aux/IAA genes of Chinese kale responded to IAA, abscisic acid (ABA) and brassinosteroids (BR) treatments, which suggested that the Aux/IAA gene family was probably involved in plant hormone signal transduction process. Additionally, under salt stress treatment, the expression of BoIAA19-1 and BoIAA2-1 genes in Chinese kale were significantly up-regulated during the early treatment stage, indicating that Aux/IAA genes may also play roles in response to diverse environmental stresses. The results of this research lay theoretical foundations for further exploring the function of the Aux/IAA gene family in the growth and resistance balance of Chinese kale.

Key words: Chinese kale Aux/IAA gene family bioinformatics analysis expression analysis

收稿日期: 2020-10-22 出版日期: 2021-06-25

CLC:

S 635.9

基金资助: 浙江省万人计划科技创新领军人才项目(2018R52026);农业农村部转基因专项(2016ZX08009003-001)

通讯作者: 黄凯美,汪俏梅 E-mail: 3170100567@zju.edu.cn;haungkm@163.com;qmwang@zju.edu.cn

作者简介: 王尤轩（https://orcid.org/0000-0002-9372-2103），E-mail：3170100567@zju.edu.cn

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引用本文:

王尤轩,王梦雨,李煜博,陶晗,夏楚楚,黄凯美,汪俏梅. 芥蓝Aux/IAA家族基因生物信息学与表达分析[J]. 浙江大学学报（农业与生命科学版）, 2021, 47(3): 314-324.

Youxuan WANG,Mengyu WANG,Yubo LI,Han TAO,Chuchu XIA,Kaimei HUANG,Qiaomei WANG. Bioinformatics and expression analysis of Aux/IAA family gene in Chinese kale. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(3): 314-324.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2020.10.221 或 http://www.zjujournals.com/agr/CN/Y2021/V47/I3/314

表1 qRT-PCR引物

序列编码 Sequence ID	基因 Gene	氨基酸数目 Amino acid number	分子质量 Molecular mass/ Da	等电点 pI	不稳定系数 Instability index	脂肪指数 Aliphatic index	平均亲水系数 Average hydrophilic index
＞Bol038615	BoIAA2-1	191	21 622.69	5.81	52.33	60.63	－0.713
＞Bol028707	BoIAA2-2	198	22 194.54	8.70	41.01	71.41	－0.554
＞Bol040875	BoIAA3-1	186	21 022.09	7.48	42.37	65.91	－0.560
＞Bol011602	BoIAA3-2	178	18 945.90	4.38	18.17	123.31	0.778
＞Bol009531	BoIAA4-1	191	21 622.69	5.81	52.33	60.63	－0.713
＞Bol009871	BoIAA5-1	128	14 445.81	7.63	57.12	86.72	－0.266
＞Bol039162	BoIAA6-1	203	22 637.07	7.62	34.75	89.66	0.299
＞Bol031746	BoIAA8-1	271	29 842.94	8.57	38.33	73.73	－0.500
＞Bol004361	BoIAA9-1	329	35 540.51	8.36	40.21	76.28	－0.385
＞Bol008065	BoIAA9-2	293	31 658.86	6.63	37.90	71.23	－0.400
＞Bol040868	BoIAA10-1	777	83 370.16	9.34	40.60	87.04	－0.087
＞Bol019649	BoIAA11-1	245	26 415.58	5.47	43.92	72.04	－0.473
＞Bol033544	BoIAA11-2	380	41 675.44	6.18	38.16	74.34	－0.339
＞Bol040903	BoIAA12-1	230	25 408.79	9.57	39.78	64.43	－0.770
＞Bol018343	BoIAA12-2	427	46 214.79	9.45	35.67	81.80	－0.277
＞Bol027258	BoIAA13-1	236	25 453.83	9.20	31.29	71.10	－0.555
＞Bol037015	BoIAA13-2	247	26 849.49	9.35	33.26	70.65	－0.582
＞Bol001436	BoIAA15-1	177	20 113.24	7.62	49.15	81.47	－0.264
＞Bol002707	BoIAA16-1	277	30 267.84	9.30	28.07	76.35	－0.374
＞Bol003120	BoIAA16-2	231	25 139.77	8.64	31.57	69.26	－0.400
＞Bol019495	BoIAA18-1	253	28 219.75	9.61	45.10	62.37	－0.911
＞Bol002014	BoIAA18-2	224	25 006.05	9.73	50.50	59.73	－0.745
＞Bol011172	BoIAA19-1	177	19 448.22	5.95	47.76	70.40	－0.441
＞Bol034711	BoIAA19-2	197	21 826.94	5.23	43.17	72.59	－0.393
＞Bol021812	BoIAA20-1	174	19 325.41	4.77	48.03	65.52	－0.438
＞Bol036925	BoIAA26-1	268	30 106.10	9.04	48.65	65.49	－0.894
＞Bol034783	BoIAA26-2	236	26 472.57	6.61	42.55	65.21	－0.967
＞Bol019603	BoIAA27-1	275	30 134.07	8.73	49.02	63.13	－0.599
＞Bol021102	BoIAA27-2	230	25 273.68	8.41	47.57	65.70	－0.511
＞Bol022320	BoIAA28-1	180	20 905.83	9.12	52.73	80.00	－0.653
＞Bol032479	BoIAA28-2	175	20 024.79	8.99	44.03	80.63	－0.679
＞Bol017925	BoIAA29-1	191	22 181.01	5.88	44.78	65.81	－0.705
＞Bol033768	BoIAA29-2	191	22 006.65	5.22	49.78	64.76	－0.646
＞Bol045689	BoIAA30-1	177	20 123.18	4.73	70.69	62.77	－0.634
＞Bol014397	BoIAA30-2	172	19 376.50	4.90	68.62	70.23	－0.477
＞Bol038661	BoIAA31-1	128	14 329.09	9.33	34.88	73.05	－0.586
＞Bol022926	BoIAA31-2	157	18 134.68	8.36	46.15	75.03	－0.527
＞Bol007955	BoIAA32-1	188	21 434.09	7.78	29.40	73.56	－0.634
＞Bol012219	BoIAA33-1	174	19 128.98	4.57	37.66	72.24	－0.502
＞Bol014224	BoIAA33-2	175	19 575.91	6.97	43.88	75.71	－0.603
＞Bol038104	BoIAA34-1	164	19 037.51	6.60	25.41	68.78	－0.668

表2 芥蓝Aux/IAA家族信息及理化性质

图1 芥蓝Aux/IAA基因家族的染色体定位

图2 芥蓝Aux/IAA蛋白家族系统进化树分析

图3 芥蓝Aux/IAA家族蛋白质保守序列基序分析

图4 Aux/IAA蛋白质保守结构域Ⅰ、Ⅱ、Ⅲ、Ⅳ的结构

图5 部分Aux/IAA基因家族成员在植物激素处理下的定量表达情况CK：0 h，清水处理。

图6 部分Aux/IAA基因家族成员在盐胁迫处理下的定量表达情况CK：0 h，清水处理。*表示与对照组（CK）相比在P＜0.05水平差异有统计学意义。

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