Please wait a minute...
浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2018, Vol. 44 Issue (5): 565-572    DOI: 10.3785/j.issn.1008-9209.2017.06.151
作物栽培与生理     
多效唑对毛竹实生苗抗旱性的影响及机制
邵珊璐，杨丽芝，陶晨悦，何安国，应叶青*
（浙江农林大学，省部共建亚热带森林培育国家重点实验室，杭州 311300）
Effects and mechanism of paclobutrazol on drought resistance of Phyllostachys edulis seedlings
SHAO Shanlu, YANG Lizhi, TAO Chenyue, HE Anguo, YING Yeqing* 
(State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China)
 全文: PDF(924 KB)   HTML ( ) 
摘要： 为探讨多效唑对毛竹抗旱性的影响，进行了多效唑质量浓度为0 和40 mg/L、基质相对含水量为30%（干旱胁迫）和70%（正常供水），共4 个处理的毛竹实生苗盆栽试验。在干旱胁迫的第7 天、14 天和21 天测定各处理的实生苗膜脂过氧化酶和抗氧化酶活性及渗透调节物质含量，在干旱胁迫第21 天测定苗高增量、生物量和叶片相对含水量。结果表明：相对于正常供水，干旱胁迫显著降低了毛竹实生苗的苗高增量、总生物量、叶片相对含水量及过氧化氢酶（catalase, CAT）和过氧化物酶（peroxidase, POD）活性，显著提高了根冠比、丙二醛（malondialdehyde, MDA）、脯氨酸（Pro）含量和超氧化物歧化酶（superoxide dismutase, SOD）活性。在干旱胁迫下，施加多效唑显著降低了苗高增量，使其达到最低值1.50 cm，而根冠比达到最大值1.35；多效唑处理可显著提高叶片相对含水量、脯氨酸含量及SOD、POD、CAT活性，显著降低MDA含量。在正常供水条件下，多效唑处理的效应与干旱胁迫下的作用类似，但作用强度不及后者。综上可见，在干旱胁迫下，多效唑处理通过提高毛竹实生苗的根冠比、叶片相对含水量、脯氨酸含量和抗氧化酶活性，降低总生物量和叶片丙二醛含量而增强其抗旱性。
关键词： 干旱胁迫;  多效唑;  毛竹实生苗;  抗旱能力    
Abstract: To reveal the mechanism of paclobutrazol (PP333) on drought resistance of Phyllostachys edulis seedlings, we set the PP333 concentrations with 0 and 40 mg/L and the relative water contents with 30% (drought stress) and 70% (normal water supply), and a total of four treatments in pot experiments. Membrane lipid peroxidases and the activities of antioxidant enzymes and contents of osmotic adjustment substances were determined at the 7th, 14th and 21st days under the drought stress, and the determination of height increment of seedlings, total biomass and leaf relative water content were conducted at the 21st day under the drought stress. The results showed that compared with the normal water supply, drought stress significantly reduced the height increment, total biomass, leaf relative water content, and the activities of catalase (CAT) and peroxidase (POD), but significantly increased the root shoot ratio, malondialdehyde (MDA) content, proline (Pro) content and superoxide dismutase (SOD) activity of P. edulis seedlings. Under the drought stress, the application of PP333 significantly reduced the seedling height increment to the lowest value of 1.50 cm, while the root shoot ratio reached the maximum value of 1.35. The application of PP333 could significantly reduced MDA content and improved the leaf relative water content, proline content, and the activities of SOD, POD and CAT. Under the normal water supply condition, the effect of PP333 was similar to that under the drought stress, while its efficacy was poor than that of the latter. In conclusion, the application of PP333 can increase the relative water content, root shoot ratio, osmotic adjustment substance (Pro) content and antioxidant enzyme activities, and decrease the MDA content to improve the drought resistance of P. eduli seedlings.
Key words: drought stress    paclobutrazol (PP333)    Phyllostachys edulis seedlings    drought resistance
出版日期: 2018-10-30
CLC:  Q 945.78  
基金资助: “十三五”国家重大研发计划“竹资源全产业链增值增效技术集成与示范”（2016YFD0600900）。
通讯作者: 应叶青(https://orcid.org/0000-0002-8113-8022)     E-mail: yeqing@zafu.edu.cn
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　
邵珊璐
杨丽芝
陶晨悦
何安国
应叶青

引用本文:

邵珊璐, 杨丽芝, 陶晨悦, 何安国, 应叶青. 多效唑对毛竹实生苗抗旱性的影响及机制[J]. 浙江大学学报（农业与生命科学版）, 2018, 44(5): 565-572.

SHAO Shanlu, YANG Lizhi, TAO Chenyue, HE Anguo, YING Yeqing. Effects and mechanism of paclobutrazol on drought resistance of Phyllostachys edulis seedlings. Journal of Zhejiang University (Agriculture and Life Sciences), 2018, 44(5): 565-572.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2017.06.151    或    http://www.zjujournals.com/agr/CN/Y2018/V44/I5/565

[1] 王一航，赵路遥，王国明，朱爱意. 舟山新木姜子幼苗对干旱胁迫的生理响应[J]. 浙江大学学报(农业与生命科学版), 2017, 43(5): 543-551.
[2] 辛福梅,贾黎明,杨小林,臧建成. 干旱胁迫对拉萨半干旱河谷主要灌木树种耗水及光合的影响[J]. 浙江大学学报(农业与生命科学版), 2016, 42(5): 617-.
[3] 辛福梅，杨小林，赵垦田，罗桑卓玛. 干旱胁迫对拉萨半干旱河谷主要乔木树种幼树耗水及光合特性的影响[J]. 浙江大学学报(农业与生命科学版), 2016, 42(2): 199-208.
[4] 许庆龙，刘晓敏，徐小兵，李晴晴，张红，肖家欣. 4种丛枝菌根真菌对南高丛蓝莓抗旱性的影响[J]. 浙江大学学报(农业与生命科学版), 2016, 42(04): 427-434.
[5] 陈方永1,2*， 倪海枝1， 王引1， 任正初1， 刘继红2， 王一光3. 多效唑对杨梅枯枝病的影响及防治[J]. 浙江大学学报(农业与生命科学版), 2014, 40(6): 653-660.
[6] . 干旱胁迫对4种砧木樱桃嫁接苗光合生理的影响[J]. 浙江大学学报(农业与生命科学版), 2012, 38(5): 585-592.
[7] 张志军　 李会珍　 姚宏亮　 周伟军. 多效唑对马铃薯试管苗生长和块茎形成的影响[J]. 浙江大学学报(农业与生命科学版), 2004, 30(3): 318-322.
[8] 郭延平　 李嘉瑞. 干旱胁迫下杏叶片膜脂脂肪酸组分和乙烯释放及LOX活性的变化[J]. 浙江大学学报(农业与生命科学版), 2002, 28(5): 513-517.