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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2016, Vol. 42 Issue (2): 179-189    DOI: 10.3785/j.issn.1008-9209.2015.05.041
农业科学     
云烟87突变体新品系的特征特性及其DNA指纹图谱鉴定
陈婷婷1，李显富2，贾春雷1，安雪琴2，朱俊1，肖立增1，戴秀梅1，张建奎1*
1.西南大学烟草科学研究所/重庆烟草科学研究所/南方山地农业教育部工程研究中心，重庆 400715；2.贵州省烟草公司铜仁市公司，贵州 铜仁 554300
Characteristics of new flue-cured tobacco strains developed from Yunyan87 mutants and their DNA fingerprint identification.
CHEN Tingting1, LI Xianfu2, JIA Chunlei1, AN Xueqin2, ZHU Jun1, XIAO Lizeng1, DAI Xiumei1, ZHANG Jiankui1*
(1. Tobacco Science Research Institute/Chongqing Tobacco Science Research Institute/Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, China; 2. Tongren Branch of Guizhou Tobacco Corporation, Tongren 554300, Guizhou, China)
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摘要： 为进一步提高目前生产上推广面积最大的烤烟品种云烟87的产量和品质，从云烟87自然变异群体中经系统选择育成2个突变体新品系T24和凹叶烟，对其特征特性进行鉴定，为其生产应用和品种鉴别与保护提供依据。田间试验以主栽烤烟品种云烟87和K326为对照，对T24和凹叶烟的植物学性状、农艺性状、产量和品质进行鉴定；利用简单重复序列(simple sequence repeat, SSR)分子标记，构建T24、凹叶烟及与其亲缘关系较近的云烟87、K326及其突变系片片黄、云烟97及其突变系M97-1、烤烟新品系09-1和客田烟共9个品种（系）的DNA指纹图谱。结果表明：1）T24和凹叶烟的株型、植物学特征及大田生育期与云烟87相似，但有效叶片数、叶片长宽、茎围、株高都超过了云烟87；两者的产量分别为2 247.38和2 195.62 kg/hm2，产值为53 657.56和52 586.47元/hm2，上中等烟率为90.59%和90.38%，均高于对照；两者的烟叶还原糖、总糖、淀粉、总植物碱含量等均达到优质烟叶标准；感官评吸评价T24为80.0分，高于云烟87（76.3分）和K326（77.7分），凹叶烟为75.3分。2）从14对SSR的核心引物中筛选出重复性好、多态性好、品种鉴别效率高的4对引物PT20213、PT30028、PT30224、PT30399，在T24、凹叶烟、云烟87、K326等9个品种中扩增出了18条多态性条带；选择其中7个谱带构建了这9个烟草品种的指纹数据库，据此绘制了DNA指纹图谱，建立了这9个品种的鉴别流程，即用至少3对引物可将这9个品种完全鉴别开。上述结果表明：T24的产量和品质均优于云烟87，具有良好的生产应用潜力；构建的参试品种的特异性指纹图谱，可作为品种鉴别的依据。
Abstract: Yunyan87 was a flue-cured tobacco variety with largest-scale cultivation in China. To further improve the yield and quality of Yunyan87, two new mutant strains named T24 and Aoyeyan were developed from the natural mutants of Yunyan87 using selection breeding method. The characteristics and DNA fingerprint of the two new mutant strains were identified to provide basis for variety application, identification and protection. The field tests were performed to identify the botanic characters, agronomic characteristics, yield and quality of T24 and Aoyeyan in Tongren City of Guizhou Province from 2011 to 2013, using the leading flue-cured tobacco cultivars Yunyan87 and K326 as control varieties. Furthermore, simple sequence repeat (SSR) molecular marker technology was used to establish the DNA fingerprint maps of T24, Aoyeyan and their genetically related varieties Yunyan87, K326, Yunyan97 and four new mutant lines and strains (M97-1, Pianpianhuang, 09-1, Ketianyan), altogether nine accessions. The results were as follows: 1) The botanic characteristics, plant shape and growth duration in field of T24 and Aoyeyan were similar with Yunyan87, but their effective leaf numbers, leaf length and width, stem circumference, plant height all exceeded Yunyan87. The yields of T24 and Aoyeyan were 2 247.38 and 2 195.62 kg/hm2, with production value of 53 657.56 and 52 586.47 Yuan RMB/hm2, and the rates of superior and middle grade leaves were 90.59% and 90.38%, respectively, which all exceeded control varieties。 The contents of reducing sugar, total sugar, starch, total plant nicotine of T24 and Aoyeyan were all up to the high quality standard of flue-cured tobacco leaves. The sensory taste score of T24 was 80.0, higher than Yunyan87 and K326, with scores of 76.3 and 77.7, respectively. The sensory taste score of Aoyeyan was 75.3. 2) Four SSR primers, i.e. PT20213, PT30028, PT30224 and PT30399 were selected from 14 core primers, which gave stable and polymorphic amplification profiles and had efficient identification ability to tobacco varieties. Eighteen polymorphic DNA fragments of all nine varieties including T24, Aoyeyan, Yunyan87, K326, Yunyan97, M97-1, Pianpianhuang, 09-1, Ketianyan were amplified, among which seven specific bands were selected to encode the fingerprint database. Fingerprint map of the nine varieties were drawn according to the fingerprint database. Each variety had a distinctive fingerprint map, therefore could be identified each other by at least three SSR primers, and the identification process of the nine varieties was established. In conclusion, the yield and quality of T24 are higher than Yunyan87, so T24 has a great application potential. The distinctive fingerprint map of the tested varieties can provide basis for the variety identification and protection.
出版日期: 2016-03-20
CLC:  S 572  
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陈婷婷
李显富
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安雪琴
朱俊
肖立增
戴秀梅
张建奎

引用本文:

陈婷婷，李显富，贾春雷，安雪琴，朱俊，肖立增，戴秀梅，张建奎. 云烟87突变体新品系的特征特性及其DNA指纹图谱鉴定[J]. 浙江大学学报(农业与生命科学版), 2016, 42(2): 179-189.

CHEN Tingting, LI Xianfu, JIA Chunlei, AN Xueqin, ZHU Jun, XIAO Lizeng, DAI Xiumei, ZHANG Jiankui. Characteristics of new flue-cured tobacco strains developed from Yunyan87 mutants and their DNA fingerprint identification.. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 42(2): 179-189.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2015.05.041    或    http://www.zjujournals.com/agr/CN/Y2016/V42/I2/179

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