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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2015, Vol. 41 Issue (03): 261-268    DOI: 10.3785/j.issn.1008-9209.2014.11.041
生物科学与技术     
水稻低纤维突变体LCM527-1性状鉴定、遗传分析与基因定位
童杰鹏,童川,王艳,任三娟,沈圣泉*
浙江大学原子核农业科学研究所,杭州310029
Character identification, genetic analysis and gene mapping of a low cellulose mutant LCM527-1 in rice
Tong Jiepeng, Tong Chuan, Wang Yan, Ren Sanjuan, Shen Shengquan*
(Institute of Nuclear Agricultural Sciences, Zhejiang University,  Hangzhou  310029, China)
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摘要： 水稻茎秆机械强度是抗倒伏能力强弱的一项重要指标。为了深入研究水稻茎秆机械强度和抗倒伏特性的内在生理、生化、遗传和分子等方面机制,经400 Gy60Co γ射线诱变和筛选,获得了一个特异性低纤维突变体LCM527-1。经考查,该突变体与野生型品种527（WT）相比,除整个生长期间表现出茎秆、叶片、叶鞘、穗颈和枝梗均脆性及极易折断外,还伴随有生长势偏弱、植株变矮、穗数减少、剑叶和穗变短等形态特性。抗倒伏指数、茎秆单位长度的鲜质量和干质量以及倒一节和倒二节抗折断力均明显降低,茎秆中Ca、Si、K等重要组成元素显著减少,茎秆纤维素含量显著下降,半纤维素含量增高。遗传分析表明,该低纤维突变性状受单隐性核基因控制（暂名为lcm527-1）,利用SSR分子标记将lcm527-1基因定位于2号染色体的RM3774和RM1092之间,遗传距离分别为10.6 cM和5.1 cM。这些结果将为今后研究低纤维突变及其精细定位提供依据,也为深入研究水稻抗倒伏机制提供分子生物学支持。
Abstract: Low cellulose mutant in rice is a common type of crop culm mutants as reported in Arabidopsis, maize and barley previously. These mutants have obvious traits of fragility in stem and leaf, low mechanical strength and weaker breaking-resistance. A low cellulose mutant 527-1 (LCM527-1) obtained from the offspring of a rice variety 527 by 400 Gy60Co γ irradiation was applied to study it’s biological phenotype, genetic traits and mutant gene, attempting to find reasons for low cellulose mutation and rice lodging. It exhibited fragility with continuity and integrity during the whole period of rice growth. In the process of experiment, some main investigations were made to identify the variation in biological expression, genetic analysis and gene mapping, concerning phenotype observation, agronomic traits, rice quality, chemical mineral elements, inheritance pattern and location of mutation gene. Four crosses were made to inspect the inheritance pattern and genetic stability between the low cellulose mutant527-1 (female parent) and the wild-type 527, Zhe7954, 9311, Minghui63 (male parents). After then, 505 pairs of SSR molecular markers were chosen to map the mutant gene on chromosome, and three markers: RM3774, RM1092 and RM530 presented polymorphism difference. These mapping populations were derived from a cross between LCM527-1 and Zhe7954. The mutant LCM527-1 showed dramatically decreased content of cellulose, high lodging index, big changes in some agronomic traits and mineral elements content. Genetic analysis indicated the character of mutant  LCM527-1 was controlled by a single recessive gene. And gene lcm527-1 was roughly located between SSR marker RM3774 and RM1092 on chromosome 2 with genetic distances of 10.6 cM and 5.1 cM, respectively. Above all, it was concluded that mutant LCM527-1 was of great importance for research of mechanical strength, especially the ability of lodging resistance. The mutant traits appeared single locus mutation and inheritable stably, suggesting some specific traits of LCM527-1 might be controlled by this mutant gene lcm527-1 and resulted in some related changes in agronomic traits and mineral elements content. These results are helpful to make a fine mapping and its gene cloning in the fragile mutant; meanwhile, they could also lay a theoretical basis on molecular biology mechanism of rice lodging resistance.  
出版日期: 2015-05-20
CLC:  Q 343.17  
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童杰鹏
童川
王艳
任三娟
沈圣泉

引用本文:

童杰鹏,童川,王艳,任三娟,沈圣泉. 水稻低纤维突变体LCM527-1性状鉴定、遗传分析与基因定位[J]. 浙江大学学报(农业与生命科学版), 2015, 41(03): 261-268.

Tong Jiepeng, Tong Chuan, Wang Yan, Ren Sanjuan, Shen Shengquan. Character identification, genetic analysis and gene mapping of a low cellulose mutant LCM527-1 in rice. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(03): 261-268.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2014.11.041    或    http://www.zjujournals.com/agr/CN/Y2015/V41/I03/261

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