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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2013, Vol. 39 Issue (6): 591-599    DOI: 10.3785/j.issn.1008-9209.2013.01.081
生物科学与技术     
环境因子变化对褐飞虱非特异性酯酶活性的影响
郭燕， 巨青松， 姚洪渭*， 蒋明星， 叶恭银， 程家安
（浙江大学昆虫科学研究所/水稻生物学国家重点实验室/农业部作物病虫分子生物学重点开放实验室，杭州 310058）
Effect of environmental factor changes on the activity of nonspecific esterase in brown planthopper, Nilaparvata lugens Stl (Hemiptera: Delphacidae)
GUO Yan, JU Qingsong, YAO Hongwei*, JIANG Mingxing, YE Gongyin, CHENG Jiaan
(Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects / State Key Laboratory of Rice Biology / Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China)
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摘要： 为了探明酯酶在褐飞虱（Nilaparvata lugens Stl）快速适应环境变化中的功能与机制，分别就水稻品种、施氮水平和温度等环境因子对褐飞虱不同种群非特异性酯酶活性的影响进行研究.结果表明：褐飞虱不同种群的酯酶活性存在极显著差异，其中以中国广西种群最高，分别是中国浙江种群和泰国彭世洛种群的2.24倍和1.24倍.将褐飞虱转接到不同水稻品种上取食后，浙江种群和彭世洛种群酯酶活性显著升高，广西种群酯酶活性除在水稻品种TN1上取食后显著升高外，均被抑制而降低.褐飞虱酯酶活性对水稻品种更换的应激响应可能与水稻籼/粳类型有关.低氮处理的稻苗可诱导浙江种群和彭世洛种群酯酶活性显著升高，使广西种群酯酶活性出现波动；高氮处理稻苗可诱导彭世洛种群酯酶活性显著升高，使浙江种群和广西种群酯酶活性出现显著的波动.高氮处理的不同褐飞虱种群酯酶活性均明显低于低氮处理的.温度变化能显著降低浙江种群和广西种群酯酶活性，却显著提高彭世洛种群酯酶活性.21 ℃处理的不同褐飞虱种群酯酶活性均明显低于29 ℃ 处理的.褐飞虱酯酶对不同环境因子变化的应激响应具有种群特异性，其中浙江种群和彭世洛种群之间的相似性较高，而与广西种群的相似性相对较低.说明褐飞虱酯酶及其基因可用作评价稻田生态系统对褐飞虱灾变调控功能的候选特征指标.
Abstract: The brown planthopper (BPH), Nilaparvata lugens Stl (Hemiptera: Delphacidae), is an important pest of rice in Asia. It sucks rice phloem sap, transmits rice viruses, and often causes serious loss of rice yield. Changes in modern cropping system like the increase in susceptible hybrid varieties, fertilizers, pesticides, and temperature resulted in high frequency of N. lugens outbreaks, mainly attributed to its capability of environmental adaptation. Esterases catalyze the hydrolysis of endogenous and foreign compounds containing ester bond, and play major roles in xenobiotic detoxification, pheromone degradation, neurogenesis, and regulation of development and reproduction in insects. However, the function and mechanism of N. lugens esterases in rapid adaption to environmental factors remains unclear. In this paper, the effects of environmental factors including rice variety, nitrogen application level, and temperature on the activity response of nonspecific esterase in female adults from different populations were preliminarily studied. Three N. lugens populations, collected from Zhejiang and Guangxi of China and Phitsanulok of Thailand, were respectively treated with rice varieties including TN1 (a insectsusceptible indica variety), IR64 (a local indica variety in tropical areas), Xiushui 11 (a local japonica variety in China) and Liangyou 363 (a local hybrid variety in China), nitrogen applications with low and high doses, and temperatures of 21 ℃ and 29 ℃. The nonspecific esterase activity in N. lugens populations was determined spectrophotometrically following the hydrolysis of pnitrophenyl acetate (pNPA) at 405 nm. The results showed that there were highly significant effects of rice varieties, nitrogen application levels, and temperatures on the esterase activity in N. lugens populations, respectively. The esterase activities in N. lugens populations were significantly different, whereas the activity of Guangxi population was about 2.24 times and 1.24 times higher than that in Zhejiang and Phitsanulok populations, respectively. In contrast to significant increase in esterase activities of Zhejiang and Phitsanulok populations after transferred feeding on different varieties, the esterase activity in Guangxi population was mainly inhibited though it increased markedly after transferred feeding on TN1. It was indicated that the changes of esterase activity of N. lugens in response to host shifting might be related to indica and japonicatypes of rice. The esterase activity was significantly induced in Zhejiang and Phitsanulok populations after transferred feeding on the rice treated with lowdose nitrogen, but fluctuated markedly in Guangxi population. Feeding on the rice treated with highdose nitrogen resulted in an increased esterase activity in Phitsanulok population while fluctuated in Zhejiang and Guangxi populations. The esterase activities in N. lugens populations on the rice treated with highdose nitrogen were lower than those on the rice treated with lowdose nitrogen. The temperature changes led to the significant decrease of the esterase activity in Zhejiang and Guangxi populations, but led to the marked increase in Phitsanulok population. The esterase activities in N. lugens populations at 21 ℃ were lower than those at 29 ℃. It is concluded that the response of esterase activity in N. lugens to different environmental factors is populationspecific. Compared with Guangxi population, Zhejiang population has a higher similarity with Phitsanulok population. It is suggested that the esterase and its genes can be used as a candidate character for evaluation of regulatory function of the paddy ecosystem on rice planthopper outbreak.
出版日期: 2013-11-20
CLC:  S 435.112.3  
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郭燕
巨青松
姚洪渭*
蒋明星
叶恭银
程家安

引用本文:

郭燕， 巨青松， 姚洪渭*， 蒋明星， 叶恭银， 程家安. 环境因子变化对褐飞虱非特异性酯酶活性的影响[J]. 浙江大学学报(农业与生命科学版), 2013, 39(6): 591-599.

GUO Yan, JU Qingsong, YAO Hongwei*, JIANG Mingxing, YE Gongyin, CHENG Jiaan. Effect of environmental factor changes on the activity of nonspecific esterase in brown planthopper, Nilaparvata lugens Stl (Hemiptera: Delphacidae). Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(6): 591-599.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2013.01.081    或    http://www.zjujournals.com/agr/CN/Y2013/V39/I6/591

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