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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (4): 400-406    DOI: 10.3785/j.issn.1008-9209.2012.04.007
生物科学与技术     
低温胁迫对铁皮石斛抗坏血酸过氧化物酶活性及丙二醛和脯氨酸含量的影响
谭艳玲， 张艳嫣， 高冬冬， 陈丹， 沈伟桥， 周建华， 黄冲平
浙江大学 农业与生物技术学院 作物科学研究所，浙江 杭州 310058
Effects of chilling stress on ascorbate peroxidase activity, malondialdehyde and proline contents of Dendrobium candidum
TAN Yanling, ZHANG Yanyan, GAO Dongdong, CHEN Dan, SHEN Weiqiao, ZHOU Jianhua, HUANG Chongping
Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
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摘要： 为研究不同铁皮石斛(Dendrobium candidum)品种在低温胁迫及恢复生长过程中的生理反应，探讨耐寒性指标，了解新品系的耐寒性强弱，将‘ZD-1’、‘ZD-2’、‘ZD-3’ 3个新品系进行低温(5、0和-2 ℃)处理6、12和18 h及恢复生长处理后，分别测定其抗坏血酸过氧化物酶(ascorbate peroxidase, APX)活性、丙二醛(malondialdehyde, MDA)及脯氨酸含量。结果表明：APX活性、MDA和脯氨酸含量均随处理温度的降低和时间的延长而上升；经0 ℃处理、恢复生长10 d后，‘ZD-3’恢复正常生长；‘ZD-2’除0 ℃/18 h外其他各处理均恢复正常生长；‘ZD-1’则在短期内难以恢复到正常水平。试验还表明：APX活性对温度变化敏感，可作为鉴定铁皮石斛耐寒性的指标；MDA和脯氨酸含量也表现出一定的规律性，可作为铁皮石斛耐寒性鉴定的辅助指标。3个品种的耐寒性强弱为：ZD-3>ZD-2>ZD-1。
Abstract: The aim was to understand the physiological responses of chilling stress and regrowth treatment on three Dendrobium candidum new varieties (‘ZD-1’, ‘ZD-2’, ‘ZD-3’). The ascorbate peroxidase (APX) activity, malondialdehyde (MDA) and proline contents in the leaves were determined at a range of different temperatures 5, 0, -2 ℃ for 6, 12, 18 h and in recovery growth for 5 and 10 days, respectively. The results showed that the APX activity, MDA and proline contents in the leaves of three new varieties were enhanced along with the increase of low temperature stress and the time prolongation except for -2 ℃/12 h and 18 h. Based on the measured indices of tenday recoveries, it was demonstrated that ‘ZD-3’ plants had a strong and positive response to chilling stress, and could shortly recover from the 0 ℃ treatments. Compared with the CK, the three indices of ‘ZD-2’ plants showed no significant differences after recovering from the 0 ℃ treatments during tendays except for 0 ℃/18 h treatment. The variety ‘ZD-1’ had a poor response, and had a serious damage among the three varieties. It is concluded that under chilling stress, the significant changes in APX activity can be used as an efficient and robust index of chilling resistance in D. candidum. As MDA and proline contents also responded to chilling stress by bringing certain regularity changes, they can also be used as one of the two auxiliary identification indices of chilling resistance. It was obtained that chilling resistance of three new varieties was decreased in the order of ZD-3, ZD-2 and ZD-1.
出版日期: 2012-07-25
CLC:  S567.23  
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谭艳玲
张艳嫣
高冬冬
陈丹
沈伟桥
周建华
黄冲平

引用本文:

谭艳玲， 张艳嫣， 高冬冬， 陈丹， 沈伟桥， 周建华， 黄冲平. 低温胁迫对铁皮石斛抗坏血酸过氧化物酶活性及丙二醛和脯氨酸含量的影响[J]. 浙江大学学报(农业与生命科学版), 2012, 38(4): 400-406.

TAN Yanling, ZHANG Yanyan, GAO Dongdong, CHEN Dan, SHEN Weiqiao, ZHOU Jianhua, HUANG Chongping. Effects of chilling stress on ascorbate peroxidase activity, malondialdehyde and proline contents of Dendrobium candidum. , 2012, 38(4): 400-406.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.04.007    或    http://www.zjujournals.com/agr/CN/Y2012/V38/I4/400

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