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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (3): 293-298    DOI: 10.3785/j.issn.1008-9209.2012.03.010
生物科学与技术     
二氢杨梅素诱导MDA-MB-231细胞凋亡
周防震1,2,3， 张晓元1， 孙奋勇4， 郭勇2
1.华南理工大学 工业技术研究总院,广东 广州510641；2.华南理工大学 生物科学与工程学院，广东 广州510006；
3.湖北民族学院 生物科学与技术学院，湖北 恩施445000；4.上海市第十人民医院 检验科，上海200072)
MDA-MB-231 cell apoptosis in vitro induced by dihydromyricetin.
ZHOU Fang zhen1,2,3, ZHANG Xiaoyuan1, SUN Fenyong4, GUO Yong2
1. Industrial Technology Research Institute, South China University of Technology, Guangzhou  510641, China; 2. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou  510006, China;3. School of Biological Science and Technology, Hubei Institute for Nationalities， Enshi, Hubei 445000, China; 4. Department of Clinical Laboratory, Shanghai Tenth People’s Hospital, Shanghai 200072, China
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摘要： 为了探讨二氢杨梅素（dihydromyricetin，DMY）诱导人乳腺癌MDA-MB-231细胞凋亡的机制，利用倒置显微镜观察细胞形态变化，用流式细胞仪检测DMY对MDA-MB-231细胞的致凋亡能力和细胞内钙离子浓度和线粒体膜电位（ΔΨm）的改变，用蛋白质印迹（Western blotting）分析检测半胱氨酸天冬氨酸蛋白酶-3（caspase-3）和半胱氨酸天冬氨酸蛋白酶-9（caspase-9）的变化。结果表明：MDA-MB-231细胞随DMY质量浓度加大而变圆变小，细胞存活数减少；流式细胞仪检测显示随DMY质量浓度的增加，DMY对MDA-MB-231细胞有促凋亡作用，胞内钙离子浓度逐渐增加，而线粒体跨膜电位（ΔΨm）逐渐减少；Western blotting结果显示DMY可诱导MDA-MB-231细胞中caspase-3和caspase-9蛋白质活化，并呈现浓度依赖关系。表明DMY可通过线粒体途径诱导MDA-MB-231细胞凋亡。
Abstract: To investigate the effect of dihydromyricetin (DMY) induction on MDA-MB-231 cell apoptosis and its mechanism, the changes of cell morphological characteristics were observed under inverted microscope. Apoptosis, intracellular Ca2+ level and the mitochondrial innermembrane potential (ΔΨm) were analyzed by flow cytometry (FCM). Western blotting was employed to detect the expression of caspase-3 and caspase-9. The results showed that the cell size and volume became smaller with increasing concentration of DMY. Increased Sub-G1 peak and intracellular Ca2+ level, as well as the decreased mitochondrial ΔΨm in MDA-MB-231 cells were obviously  observed in a dosedependent manner. Western blotting analysis showed that DMY dosedependently increased the expression of caspase-3 and caspase-9 in MDA-MB-231 cells. These results indicate that DMY can induce MDA-MB-231 cell apoptosis by the mitochondrial pathway.
出版日期: 2012-05-24
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周防震
张晓元
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郭勇

引用本文:

周防震， 张晓元， 孙奋勇， 郭勇. 二氢杨梅素诱导MDA-MB-231细胞凋亡[J]. 浙江大学学报(农业与生命科学版), 2012, 38(3): 293-298.

ZHOU Fangzhen, ZHANG Xiaoyuan, SUN Fenyong, GUO Yong. MDA-MB-231 cell apoptosis in vitro induced by dihydromyricetin.. , 2012, 38(3): 293-298.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.03.010    或    http://www.zjujournals.com/agr/CN/Y2012/V38/I3/293

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