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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2013, Vol. 14 Issue (4): 289-298    DOI: 10.1631/jzus.B1200278
Articles     
Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability
Zhang-yue Song, Jing-luan Tian, Wei-zhe Fu, Lin Li, Ling-hong Lu, Lian Zhou, Zhi-hui Shan, Gui-xiang Tang, Hui-xia Shou
College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
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Abstract  The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation. Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation system. In this study, twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection, regeneration capacity, and stable transgenic efficiency. Three genotypes, Yuechun 04-5, Yuechun 03-3, and Tianlong 1, showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental system. For the Tianlong 1, the average stable transformation efficiency is 4.59%, higher than that of control genotypes (Jack and Williams 82), which is enough for further genomic research and genetic engineering. While polymerase chain reaction (PCR), LibertyLink strips, and β-glucuronidase (GUS) staining assays were used to detect the insertion and expression of the transgene, leaves painted with 135 mg/L Basta could efficiently identify the transformants.

Key words： Soybean      Agrobacterium-mediated transformation      Genotypes      Stable transgenic efficiency     
Received: 12 October 2012      Published: 03 April 2013
CLC:  Q789  
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Zhang-yue Song
Hui-xia Shou
Jing-luan Tian
Wei-zhe Fu
Lin Li
Ling-hong Lu
Lian Zhou
Zhi-hui Shan
Gui-xiang Tang
Cite this article:

Zhang-yue Song, Jing-luan Tian, Wei-zhe Fu, Lin Li, Ling-hong Lu, Lian Zhou, Zhi-hui Shan, Gui-xiang Tang, Hui-xia Shou. Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(4): 289-298.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1200278     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2013/V14/I4/289

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