Genome-wide identification and expression analysis of <i>Dimocarpus longan</i><i>UBP</i> family and its expression analysis under drought stress

doi:10.3785/j.issn.1008-9497.2023.03.015

Journal of Zhejiang University (Science Edition)

2023, Vol. 50

Issue (3): 367-377 DOI: 10.3785/j.issn.1008-9497.2023.03.015

Life Science

Genome-wide identification and expression analysis of Dimocarpus longanUBP family and its expression analysis under drought stress

Ning TONG,Chunyu ZHANG,Xiaoqiong XU,Xiaohui CHEN,Xu SHEN,Zhongxiong LAI(

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Institute of Horticultural Biotechnology，Fujian Agriculture and Forestry University，Fuzhou 350002，China

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Abstract

Ubiquitin-specific protease (UBP) is the largest among the six families of deubiquitinating enzymes. It is widely found in eukaryotes and is highly conserved. They regulate the growth and development of various organisms through deubiquitination. In order to understand the biological function of DlUBP gene family and its role during somatic embryogenesis of Dimocarpus longan Lour. In this study, we performed genome-wide identification of DlUBP gene family members; analysed their genes structure, protein structure, promoter cis-acting elements and constructed phylogenetic tree based on the third-generation longan genome database and transcriptome database; then analyzed the expression of DlUBP under ABA treatment and drought stress. The results showed that the DlUBP gene family contains 18 members, which are divided into 10 subfamilies, the largest subfamily has four members; the number of amino acids are between 369-1 117, and the number of introns are between 2-31; There are a large number of light response elements, anaerobic induction response elements, and hormone response elements in the DlUBP promoter sequence with a prolonged procecsing time, the expression level of many DlUBP members showed a decreasing trend under abscisic acid treatment, and the expression level of many DlUBP genes increased under drought stress. It is speculated that the DlUBP gene family participates in the regulation of abscisic acid during somatic embryogenesis of longan and responses to drought stress.

Key words： Dimocarpus longan UBP gene genome-wide identification expression

Received: 16 November 2021 Published: 19 May 2023

CLC:

S 667.2

Corresponding Authors: Zhongxiong LAI E-mail: laizx01@163.com

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	Ning TONG
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	Xu SHEN
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Cite this article:

Ning TONG, Chunyu ZHANG, Xiaoqiong XU, Xiaohui CHEN, Xu SHEN, Zhongxiong LAI. Genome-wide identification and expression analysis of Dimocarpus longanUBP family and its expression analysis under drought stress. Journal of Zhejiang University (Science Edition), 2023, 50(3): 367-377.

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https://www.zjujournals.com/sci/EN/Y2023/V50/I3/367

龙眼UBP家族全基因组鉴定及其在外源胁迫下的表达分析

泛素特异蛋白酶（ubiquitin-specific protease，UBP）是去泛素化酶六大家族中最大的，广泛存在于真核生物中，保守性很高。UBP可通过去泛素化作用调节各种生物的生长发育。为了解龙眼（Dimocarpus longan Lour.）DlUBP基因家族的生物学功能及其在龙眼体胚早期发生过程中的作用，基于第3代龙眼基因组数据库与转录组数据库，对DlUBP全基因组进行鉴定，分析其基因结构、蛋白质结构域、启动子顺式元件及构建系统进化树，并对DlUBP基因在脱落酸处理和干旱胁迫处理下的表达情况进行分析。结果显示，DlUBP家族共有18个成员，分为10个亚家族，其中最大的亚家族有4个成员；各成员的氨基酸数为369~1 117个，内含子数为2~31个；在DlUBP启动子序列中存在大量光响应元件、厌氧诱导响应元件、激素响应元件，随着处理时间的增加，在脱落酸处理下，多数DlUBP基因的表达量呈降低趋势，在干旱胁迫处理下，有不少DlUBP基因的表达量升高，推测DlUBP基因在龙眼体胚早期参与脱落酸的调控并对干旱胁迫有应激反应。

关键词： 龙眼, UBP基因, 成员鉴定, 表达情况

Table 1 Real-time fluorescent quantitative PCR primers

Table 2 Characteristics of DlUBP gene family protein

Fig.1 Location of DlUBP gene on chromosome

Fig.2 Longan DlUBP family member gene structure and conservative motif distribution map

Fig.3 DlUBP family protein conserved domain analysis

Fig.4 Phylogenetic tree analysis of plantUBPfamily members

Fig.5 Promoter cis-acting elements of DlUBP gene

Fig. 6 The specific expression of different stages of somatic embryogenesis in the DlUBP gene

Fig.7 Expression of six DlUBP gene family membersunder different abscisic acid treatment timesDifferent lowercase letters indicate significant difference at 0.05 level.

Fig.8 Expression of six DlUBP gene family membersunder different drought stress treatment timesDifferent lowercase letters indicate significant difference at 0.05 level.


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