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浙江大学学报（理学版）
浙江大学学报（理学版）  2020, Vol. 47 Issue (4): 469-475    DOI: 10.3785/j.issn.1008-9497.2020.04.010
化学     
阳离子交换皂石黏土在基质辅助激光解吸电离飞行时间质谱分析中的应用
丁宇琦, 朱价, 周霞, 罗金文
浙江省食品药品检验研究院，浙江 杭州310012
Cation-exchanged smectite layers for matrix-assisted laser desorption/ionization mass spectrometry analysis
DING Yuqi, ZHU Jia, ZHOU Xia, LUO Jinwen
Zhejiang Institute for Food and Drug Control, Hangzhou 310012, China
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摘要： 将一种人工合成的无机聚合物——蒙脱石皂石黏土（smectite，Sm）应用于基质辅助激光解吸电离飞行时间质谱分析（matrix-assisted laser desorption/ionization time of flight mass spectrometry, MALDI-TOF-MS），以检测糖类化合物。 将传统的有机基质2，4，6-三羟基苯乙酮（trihydroxyacetophenone, THAP）与阳离子交换后的皂石黏土混合制备成新型复合基质，应用于糖类化合物的检测。通过比较不同的制样方法，测定不同分子直径的糖类化合物，发现由于受复合基质晶面间距的限制，只有小分子糖类化合物能进入晶面间隙充分接触有机基质并被离子化，从而实现对小分子糖类化合物的选择性检测。
关键词： 基质辅助激光解吸电离;  晶面间距;  皂石黏土;  糖类化合物    
Abstract: Smectite, a synthetic inorganic polymer with a saponite structure, was subjected to matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) for the detection of saccharides. Typical organic matrix molecules 2,4,6-trihydroxyacetophenone (THAP) were intercalated into the layer spacing of cation-exchanged smectite and the complex was used as a new matrix for laser desorption/ionization mass spectrometry. Through the comparison of different sample preparation methods as well as the measurement of saccharides with disparate grain size, it is found that only saccharides of small analyte could enter the layer and bind to THAP can be ionized because of layer spacing limitations.
Key words: smectite    matrix-assisted laser desorption/ionization    layer spacing    saccharides
收稿日期: 2018-10-12 出版日期: 2020-07-25
CLC:  O657.63  
基金资助: 浙江省科技厅科技计划项目（2018C37011）；浙江省食品药品监督管理局食品药品监管系统科技计划项目（SP201703）.
通讯作者: ORCID:http://orcid.org/0000-0003-1695-1647， E-mail: 35386375@qq. com.     E-mail: 35386375@qq. com
作者简介: 丁宇琦（1986—），ORCID:http://orcid.org/0000-0001-6420-0325 ，男，博士，工程师，主要从事仪器分析和食品检验方法的开发研究.。
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引用本文:

丁宇琦, 朱价, 周霞, 罗金文. 阳离子交换皂石黏土在基质辅助激光解吸电离飞行时间质谱分析中的应用[J]. 浙江大学学报（理学版）, 2020, 47(4): 469-475.

DING Yuqi, ZHU Jia, ZHOU Xia, LUO Jinwen. Cation-exchanged smectite layers for matrix-assisted laser desorption/ionization mass spectrometry analysis. Journal of Zhejiang University (Science Edition), 2020, 47(4): 469-475.

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https://www.zjujournals.com/sci/CN/10.3785/j.issn.1008-9497.2020.04.010    或    https://www.zjujournals.com/sci/CN/Y2020/V47/I4/469

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