In order to solve the problems of poor positioning accuracy and high power consumption of electronic tags in the traditional RFID positioning technology,a design scheme of 2.4 GHz high accuracy regional positioning system based on low frequency trigger was proposed.The scheme combined the low frequency 125 kHz trigger signal with the 2.4 GHz radio-frequency signal,the power consumption of the electronic tag was greatly reduced by the method of low frequency wake,the positioning accuracy of RFID system could be further improved by using the trigger mechanism which could adjust the trigger distance,2.4 GHz active electronic tag,125 kHz trigger and 2.4 GHz reader were designed based on the proposed scheme.The separation design of reader and trigger reduced the cost of layout.In the design of system hardware circuit,the adjustable potentiometer and digital voltage regulator were added to adjust the recognition distance and the trigger distance,these made the configuration of the equipment more convenient and enhanced the practicability of system.The testing data showed that the maximum communication distance between the reader and the tag was 120 m,standby time of active electronic tag can be about 2 years,the positioning accuracy was 1-5.2 m.The result verifies the effectiveness of the proposed scheme.