Uridine diphosphate-glucose 4-epimerase encoding gene galE affects the pathogenicity and carbon metabolism of Ralstonia pseudosolanacearum

doi:10.3785/j.issn.1008-9209.2023.09.191

Journal of Zhejiang University (Agriculture and Life Sciences)

2023, Vol. 49

Issue (5): 662-676 DOI: 10.3785/j.issn.1008-9209.2023.09.191

Special Topic: Major Bacterial and Viral Diseases in Crops

Uridine diphosphate-glucose 4-epimerase encoding gene galE affects the pathogenicity and carbon metabolism of Ralstonia pseudosolanacearum

Hong ZHANG¹(

),Zhijian LIN²,Jindong ZHU¹,Zhaomiao LIN¹,Guoliang LI¹,Yongqing XU¹,Zhonghua LIU¹,Yongxiang QIU¹,Sixin QIU¹(

)

^1.Crop Research Institute, Fujian Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China, Ministry of Agriculture and Rural Affairs/Fujian Engineering Research Center for Characteristic Upland Crops Breeding, Fuzhou 350013, Fujian, China
^2.Sanming Academy of Agricultural Sciences, Sanming 365509, Fujian, China

Download:

HTML

HTML ( )

PDF(6149KB)
Export: BibTeX | EndNote (RIS)

Abstract

In order to clarify the pathogenic function of uridine diphosphate (UDP)-glucose 4-epimerase encoding gene galE on Ralstonia pseudosolanacearum, the association between galE and the pathogenic-related phenotypes, and the gene deletion effect on physiological and biochemical metabolism of R. pseudosolanacearum were explored by constructing galE gene knock-out strain ΔgalE and its complementary strain CΔgalE. The results showed that the deletion of galE gene significantly decreased the pathogenicity of R. pseudosolanacearum on sweet potatoes and affected pathogenic-related phenotypes. The colony fluidity, swimming mobility, exopolysaccharide content and biofilm formation of ΔgalE reduced compared to those of wild-type SPRS911 and CΔgalE. In the galactose metabolism pathway, after deletion of galE gene, the expression levels of galU, pgm, and glk genes involved in the metabolism between uridine diphosphate glucose (UDPG) and glucose decreased, and D-glucose 6-phosphate accumulated. The expression levels of UDP-galactose (UDP-Gal) metabolism related genes dgoK, dgoAa, dgoAb, malZ and galM increased. The deletion of galE gene also enhanced the assimilation of malic acid in R. pseudosolanacearum. These results indicate that the galE gene has significant effects on the pathogenicity and the carbon metabolism of R. pseudosolanacearum.

Key words： Ralstonia pseudosolanacearum sweet potato blast uridine diphosphate-glucose 4-epimerase exopolysaccharide galactose metabolism carbon metabolism

Received: 19 September 2023 Published: 03 November 2023

CLC:

S432.1

Corresponding Authors: Sixin QIU E-mail: teeteeking@163.com;25273531@qq.com

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Hong ZHANG
	Zhijian LIN
	Jindong ZHU
	Zhaomiao LIN
	Guoliang LI
	Yongqing XU
	Zhonghua LIU
	Yongxiang QIU
	Sixin QIU

Cite this article:

Hong ZHANG,Zhijian LIN,Jindong ZHU,Zhaomiao LIN,Guoliang LI,Yongqing XU,Zhonghua LIU,Yongxiang QIU,Sixin QIU. Uridine diphosphate-glucose 4-epimerase encoding gene galE affects the pathogenicity and carbon metabolism of Ralstonia pseudosolanacearum. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(5): 662-676.

URL:

https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2023.09.191 OR https://www.zjujournals.com/agr/Y2023/V49/I5/662

尿苷二磷酸-葡萄糖4-差向异构酶编码基因galE影响甘薯青枯菌致病性和碳代谢

为明确尿苷二磷酸（uridine diphosphate, UDP）-葡萄糖4-差向异构酶编码基因galE的功能，通过构建甘薯青枯菌galE基因敲除菌株ΔgalE及回补菌株CΔgalE，探索galE基因与甘薯青枯菌致病相关表型间的关联及对甘薯青枯菌生理代谢的影响。结果表明：galE基因缺失显著降低了青枯菌对甘薯的致病性，并且影响了致病相关表型。ΔgalE菌落流动性、菌体泳动能力、胞外多糖含量和生物膜形成量均明显低于野生型SPRS911和CΔgalE。在半乳糖代谢途径中，galE基因缺失后，参与尿苷二磷酸葡糖与葡萄糖之间代谢的galU、pgm、glk基因表达量降低，D-葡萄糖-6-磷酸累积；与UDP-半乳糖代谢有关的dgoK、dgoAa、dgoAb、malZ和galM基因表达量升高。galE基因缺失还加强了甘薯青枯菌对于苹果酸的同化作用。上述研究结果说明，galE基因对青枯菌的致病性与碳代谢水平均有明显影响。

关键词： 甘薯青枯菌, 甘薯瘟病, 尿苷二磷酸-葡萄糖4-差向异构酶, 胞外多糖, 半乳糖代谢, 碳代谢

Table 1 Strains and plasmids used in this study

Table 2 Media used in this study

引物名称 Primer name	引物序列（5 $′$ →3 $′$ ） Primer sequence (5 $′$ →3 $′$ )	用途 Purpose
galE-UP	F: TATGACATGATTACGAATTCGAAGCCCAGCAGCAGGCA (EcoRⅠ) R: TACCCTTCACCCGATGCAGACCCGGGGCTGATC	扩增galE基因上游重组臂
galE-DO	F: TCAGCCCCGGGTCTGCATCGGGTGAAGGGTTCGA R: TCGACGGCCAGTGCCAAGCTTCAATGCCAGTGCCTTCAAGCT (HindⅢ)	扩增galE基因下游重组臂
BRgalE	F: CCCAAGCTTATGAACGAAACCATCCTGCTGACC (HindⅢ) R: TGGACTAGTCAGCCCCGGGTCTGAAAG (SpeⅠ)	扩增galE基因全长序列
qgalE	F: TTACCGTTAACCTCGGCACC R: TCGCGACGATCTCATAGGGG	实时荧光定量PCR（real-time fluorescence quantita-tive PCR, qRT-PCR）扩增
qdgoK	F: GAGAACCTCGGACTGCACAA R: TCAGGCTCGGTGATGGACAG
qdgoAa	F: TCGAGATCCCGCTCAACTCGC R: TCCGCAGCCAACTGGTCAACG
qdgoAb	F: TCAACGGCTGTGAGGAGATG R: TATGAACAGCGGGTGGTACG
qmalZ	F: CCTTGCTCGACTACGGCATC R: TCGCATCATCAGCTGGGTCT
qgalM	F: GCTCACACATCGTCCTGCT R: TCATGCTTGATGGCGAACGTC
qlacC	F: CTACTGGGCACCGTACTATCG R: TCTGAGCATCGAGGTTGACCA
qpgm	F: GTACATCGACCGCATCGTCT R: TGTGGTGGTTCGGAAAATGGC
qglk	F: CTGTCCGATTTCAAGCTGCG R: TAAGGCGAGGGCGTCGAT
qgalU	F: CGACTTCGAGGCGTATCTGT R: TCCAGCGGCAACACTTTCTTG
qgyrA	F: CGACTGGAACCGTCCCTAC R: TCCGCACGATGGTGTCATA

Table 3 Primers used in this study

Fig. 1 Structures of GALE protein andUDP-glucose 4-epimeraseA. Predicted structure of GALE protein in R. pseudosolanacearum; B. Alignment of amino acid sequences; C. Structure of UDP-glucose 4-epimerase of B. pseudomallei.

Fig. 2 Phylogenetic tree based on GALE amino acid sequences

Fig. 3 Verification of the galE gene knock-outand complementary strainsA. PCR amplification of the knock-out strain ΔgalE; B. PCR amplification of the complementary strain CΔgalE; C. qRT-PCR detection of the transcriptional levels of galE gene in SPRS911, ΔgalE andCΔgalE. M: DL2000 DNA marker. Double asterisks (**) and triple asterisks (***) indicate highly and extremely highly significant differences at the 0.01 and 0.001 probability levels, respectively (the same as below), and n=3.

Fig. 4 Colony morphologies of SPRS911, ΔgalE and CΔgalE strains on CPG-TTC plates

Fig. 5 Growth curves of SPRS911, ΔgalE and CΔgalE strains in CPG liquid media

Fig. 6 Pathogenicity of SPRS911, ΔgalE and CΔgalE strains on sweet potato leavesA. Diagram of the disease grade division; B. Disease symptoms on sweet potato leaves after 6 days post inoculation; C. Disease index on sweet potato leaves after 6 days post inoculation (n=4).

Fig. 7 Swimming mobility of SPRS911, ΔgalE and CΔgalE strainsA. Colony morphology after cultured for 36 h; B. Colony diameter change within 72 h of culture.

Fig. 8 EPS contents of SPRS911, ΔgalE and CΔgalE strainsQuadruple asterisks (****) indicate extremely highly significant differences at the 0.000 1 probability level (the same as below), and n=5.

Fig. 9 Biofilm formation in SPRS911, ΔgalE and CΔgalE strainsSingle asterisk (*) indicates significant differences at the 0.05 probability level (the same as below), and n=4.

Fig. 10 Galactose metabolism related gene expression levels of SPRS911, ΔgalE and CΔgalE strains after inoculating sweet potatoes

Fig. 11 galE gene involved galactose metabolism pathway of R. pseudosolanacearumRed letter indicates the gene expression trend as galE; green letter indicates the gene expression trend opposite to galE; gray dotted arrow indicates the intermediate process is not clear.

Fig. 12 D-glucose 6-phosphate contents of SPRS911, ΔgalE and CΔgalE strains

Table 4 Physiological and biochemical characteristics of SPRS911, ΔgalE and CΔgalE strains


[7]	刘中华,余华,方树民,等.甘薯瘟田间自然诱发鉴定及系统聚类分析[J].江西农业大学学报,2014,36(5):1066-1073. DOI:10.13836/j.jjau.2014170 LIU Z H, YU H, FANG S M, et al. The resistance evaluation of natural Ralstonia solanacearum nursery of sweetpotato varieties and cluster analysis[J]. Acta Agriculturae Universitatis Jiangxiensis, 2014, 36(5): 1066-1073. (in Chinese with English abstract) doi: 10.13836/j.jjau.2014170

[8]	张鸿,刘中华,林志坚,等.福建甘薯薯瘟病菌致病型分布和甘薯抗病品种筛选[J].江苏师范大学学报(自然科学版),2017,35(4):15-20. DOI:10.3969/j.issn.2095-4298.2017.04.004 ZHANG H, LIU Z H, LIN Z J, et al. Distribution of different pathotypes of sweetpotato Ralstonia solanacearum and selection of disease resistant sweetpotato varieties in Fujian Province[J]. Journal of Jiangsu Normal University (Natural Science Edition), 2017, 35(4): 15-20. (in Chinese with English abstract) doi: 10.3969/j.issn.2095-4298.2017.04.004

[9]	潘哲超.植物青枯菌遗传多样性及致病力分化研究[D].北京:中国农业科学院,2010. PAN Z C. Genetic diversity and pathogenicity variation of Ralstonia solanacearum [D]. Beijing: Chinese Academy of Agricultural Sciences, 2010. (in Chinese with English abstract)

[10]	卢同,种藏文,王长方,等.甘薯青枯菌的生理小种研究[J].福建省农科院学报,1996,11(1):36-40. LU T, ZHONG Z W, WANG C F, et al. A study on physiological races of Pseudomonas solanacearum in sweet potatoes[J]. Journal of Fujian Academy of Agricultural Sciences, 1996, 11(1): 36-40. (in Chinese with English abstract)

[11]	卢同,种藏文,王长方,等.甘薯青枯菌的生化型研究[J].福建省农科院学报,1990,5(1):40-44. LU T, ZHONG Z W, WANG C F, et al. Studies on biotypes in isolates of Pseudomonas solanacearum from sweet potatoes[J]. Journal of Fujian Academy of Agricultural Sciences, 1990, 5(1): 40-44. (in Chinese with English abstract)

[12]	JYOTI P, SHREE M, JOSHI C, et al. The Entner-Doudoroff and nonoxidative pentose phosphate pathways bypass glycolysis and the oxidative pentose phosphate pathway in Ralstonia solanacearum [J]. mSystems, 2020, 5(2): e00091-20. DOI: 10.1128/mSystems.00091-20 doi: 10.1128/mSystems.00091-20

[13]	KRISPIN O, ALLMANSBERGER R. The Bacillus subtilis galE gene is essential in the presence of glucose and galactose[J]. Journal of Bacteriology, 1998, 180(8): 2265-2270. DOI: 10.1128/JB.180.8.2265-2270.1998 doi: 10.1128/JB.180.8.2265-2270.1998

[1]	PRIOR P, AILLOUD F, DALSING B L, et al. Genomic and proteomic evidence supporting the division of the plant pathogen Ralstonia solanacearum into three species[J]. BMC Genomics, 2016, 17: 90. DOI: 10.1186/s12864-016-2413-z doi: 10.1186/s12864-016-2413-z

[2]	WICKER E, GRASSART L, CORANSON-BEAUDU R, et al. Ralstonia solanacearum strains from Martinique (French West Indies) exhibiting a new pathogenic potential[J]. Applied and Environmental Microbiology, 2007, 73(21): 6790-6801. DOI: 10.1128/AEM.00841-07 doi: 10.1128/AEM.00841-07

[14]	CHAI Y R, BEAUREGARD P B, VLAMAKIS H, et al. Galactose metabolism plays a crucial role in biofilm formation by Bacillus subtilis [J]. eBio, 2012, 3(4): e00184-12. DOI: 10.1128/mbio. 00184-12 doi: 10.1128/mbio. 00184-12

[15]	SHUSTER C W, RUNDELL K. Resistance of Salmonella typhimurium mutants to galactose death[J]. Journal of Bac-teriology, 1969, 100(1): 103-109.

[3]	SAFNI I, CLEENWERCK I, DE VOS P, et al. Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: proposal to emend the descriptions of Ralstonia solanacearum and Ralstonia syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii subsp. nov., R. solanacearum phylotype Ⅳ strains as Ralstonia syzygii subsp. indonesiensis subsp. nov., banana blood disease bacterium strains as Ralstonia syzygii subsp. celebesensis subsp. nov. and R. solanacearum phylotypeⅠand Ⅲ strains as Ralstonia pseudosolanacearum sp. nov.[J]. International Journal of Systematic and Evolutionary Microbiology, 2014, 64(9): 3087-3103. DOI: 10.1099/ijs.0.066712-0 doi: 10.1099/ijs.0.066712-0

[4]	FEGAN M, PRIOR P. How complex is the “Ralstonia solanacearum species complex”[M]//ALLEN C, PRIOR P, HAYWARD A C. Bacterial Wilt Disease and the Ralstonia solanacearum Species Complex. Saint Paul, MN: APS Press, 2005: 449-461.

[16]	FRY B N, FENG S, CHEN Y Y, et al. The galE gene of Campylobacter jejuni is involved in lipopolysaccharide synthesis and virulence[J]. Infection and Immunity, 2000, 68(5): 2594-2601. DOI: 10.1128/IAI.68.5.2594-2601.2000 doi: 10.1128/IAI.68.5.2594-2601.2000

[17]	ROBERTSON B D, FROSCH M, VAN PUTTEN J P M. The role of galE in the biosynthesis and function of gonococcal lipopolysaccharide[J]. Molecular Microbiology, 1993, 8(5): 891-901. DOI: 10.1111/j.1365-2958.1993.tb01635.x doi: 10.1111/j.1365-2958.1993.tb01635.x

[5]	LOWE-POWER T, AVALOS J, BAI Y L, et al. A meta-analysis of the known global distribution and host range of the Ralstonia species complex[J]. bioRxiv, 2020, 7: 189936. DOI: 10.1101/2020.07.13.189936 doi: 10.1101/2020.07.13.189936

[6]	蔡刘体,汪汉成,刘艳霞,等.青枯菌种内分型研究进展[J].生物技术通报,2013(7):20-23. DOI:10.13560/j.cnki.biotech.bull.1985.2013.07.001 doi: 10.13560/j.cnki.biotech.bull.1985.2013.07.001

[18]	NAKAO R, SENPUKU H, WATANABE H. Porphyromonas gingivalis galE is involved in lipopolysaccharide O-antigen synthesis and biofilm formation[J]. Infection and Immunity, 2006, 74(11): 6145-6153. DOI: 10.1128/IAI.00261-06 doi: 10.1128/IAI.00261-06

[19]	NIOU Y K, WU W L, LIN L C, et al. Role of galE on biofilm formation by Thermus spp.[J]. Biochemical and Bio-physical Research Communications, 2009, 390(2): 313-318. DOI: 10.1016/j.bbrc.2009.09.120 doi: 10.1016/j.bbrc.2009.09.120

[6]	CAI L T, WANG H C, LIU Y X, et al. Advance of the classification in Ralstonia solanacearum species[J]. Biote-chnology Bulletin, 2013(7): 20-23. (in Chinese with English abstract) doi: 10.13560/j.cnki.biotech.bull.1985.2013.07.001

[20]	LI C T, LIAO C T, DU S C, et al. Functional charac-terization and transcriptional analysis of galE gene encoding a UDP-galactose 4-epimerase in Xanthomonas campestris pv. campestris [J]. Microbiological Research, 2014, 169(5/6): 441-452. DOI: 10.1016/j.micres.2013.08.005 doi: 10.1016/j.micres.2013.08.005

[21]	陈书冰,许孜书,黄倩,等.甘蓝型油菜肌醇加氧酶基因家族鉴定与表达分析[J].浙江大学学报(农业与生命科学版),2023,49(4):484-496. DOI:10.3785/j.issn.1008-9209.2022.09.281 CHEN S B, XU Z S, HUANG Q, et al. Identification and expression analysis of myo-inositol oxygenase gene family in Brassica napus L.[J]. Journal of Zhejiang University (Agriculture & Life Sciences), 2023, 49(4): 484-496. (in Chinese with English abstract) doi: 10.3785/j.issn.1008-9209.2022.09.281

[22]	FAN X J, ZHAO Z W, SUN T Y, et al. The LysR-type trans-criptional regulator CrgA negatively regulates the flagellar master regulator flhDC in Ralstonia solanacearum GMI1000[J]. Journal of Bacteriology, 2020, 203(1): e00419-20. DOI: 10.1128/JB.00419-20 doi: 10.1128/JB.00419-20

[23]	张鸿,林志坚,林赵淼,等.水培法鉴定甘薯抗甘薯瘟病技术的构建[J].江苏师范大学学报(自然科学版),2022,40(3):22-26. DOI:10.3969/j.issn.2095-4298.2022.03.005 ZHANG H, LIN Z J, LIN Z M, et al. Construction of identification of sweetpotato resistance to Ralstonia solanacearum by hydroponics[J]. Journal of Jiangsu Normal University (Natural Science Edition), 2022, 40(3): 22-26. (in Chinese with English abstract) doi: 10.3969/j.issn.2095-4298.2022.03.005

[24]	陈小强,陈德局,朱育菁,等.青枯雷尔氏菌胞外多糖合成缺失突变株构建及其生物学特性[J].微生物学报,2018,58(5):926-938. DOI:10.13343/j.cnki.wsxb.20170590 CHEN X Q, CHEN D J, ZHU Y J, et al. Construction and characterization of extracellular polysaccharide deletion mutant of Ralstonia solanacearum [J]. Acta Microbiologica Sinica, 2018, 58(5): 926-938. (in Chinese with English abstract) doi: 10.13343/j.cnki.wsxb.20170590

[25]	梁欢.植物青枯菌spoT与relA基因功能研究[D].北京:中国农业科学院,2020. LIANG H. Research on function of spoT and relA genes in Ralstonia solanacearum [D]. Beijing: Chinese Academy of Agricultural Sciences, 2020. (in Chinese with English abstract)

[26]	PATRA T, KOLEY H, RAMAMURTHY T, et al. The Entner-Doudoroff pathway is obligatory for gluconate utilization and contributes to the pathogenicity of Vibrio cholerae [J]. Journal of Bacteriology, 2012, 194(13): 3377-3385. DOI: 10.1128/JB.06379-11 doi: 10.1128/JB.06379-11

[27]	GENIN S, DENNY T P. Pathogenomics of the Ralstonia solanacearum species complex[J]. Annual Review of Phytopa-thology, 2012, 50: 67-89. DOI: 10.1146/annurev-phyto-081211-173000 doi: 10.1146/annurev-phyto-081211-173000

[28]	D’HAEZE W, HOLSTERS M. Surface polysaccharides enable bacteria to evade plant immunity[J]. Trends in Micro-biology, 2004, 12(12): 555-561. DOI: 10.1016/j.tim.2004.10.009 doi: 10.1016/j.tim.2004.10.009

[29]	陈德局,张海峰,刘波,等.青枯雷尔氏菌胞外多糖研究进展[J].福建农业科技,2017(11):45-48. DOI:10.13651/j.cnki.fjnykj.2017.11.017 CHEN D J, ZHANG H F, LIU B, et al. Research process in exopolysaccharide of Ralstonia solanacearum [J]. Fujian Agricultural Science and Technology, 2017(11): 45-48. (in Chinese with English abstract) doi: 10.13651/j.cnki.fjnykj.2017.11.017

[30]	YANG L, WEI Z L, LI S L, et al. Plant secondary metabolite, daphnetin reduces extracellular polysaccharides production and virulence factors of Ralstonia solanacearum [J]. Pesticide Biochemistry and Physiology, 2021, 179: 104948. DOI: 10.1016/j.pestbp.2021.104948 doi: 10.1016/j.pestbp.2021.104948

[31]	周偲健.番茄青枯病菌(Ralstonia solanacearum)致病相关基因epsC的克隆及功能分析[D].广东,广州:华南农业大学,2019. ZHOU C J. Cloning and functional analysis of the pathogenic-related gene epsC of Ralstonia solanacearum [D]. Guangzhou, Guangdong: South China Agricultural University, 2019. (in Chinese with English abstract)

[32]	袁婷,李金豪,刘吉平.茄科劳尔氏菌复合体毒力基因及调控网络最新研究进展[J].微生物学通报,2023,50(5):2227-2248. DOI:10.13344/j.microbiol.china.220742 YUAN T, LI J H, LIU J P. Latest research progress in virulence genes and regulatory networks of Ralstonia sola-nacearum species complex[J]. Microbiology China, 2023, 50(5): 2227-2248. (in Chinese with English abstract) doi: 10.13344/j.microbiol.china.220742

[33]	LIU A, MI Z H, ZHENG X Y, et al. Exopolysaccharides play a role in the swarming of the benthic bacterium Pseudoal-teromonas sp. SM9913[J]. Frontiers in Microbiology, 2016, 7: 473. DOI: 10.3389/fmicb.2016.00473 doi: 10.3389/fmicb.2016.00473

[34]	ORGAMBIDE G, MONTROZIER H, SERVIN P, et al. High heterogeneity of the exopolysaccharides of Pseudomonas solanacearum strain GMI 1000 and the complete structure of the major polysaccharide[J]. Journal of Biological Chemistry, 1991, 266(13): 8312-8321.

[35]	ARAUD-RAZOU I, VASSE J, MONTROZIER H, et al. Detection and visualization of the major acidic exopoly-saccharide of Ralstonia solanacearum and its role in tomato root infection and vascular colonization[J]. European Journal of Plant Pathology, 1998, 104(8): 795-809.

[36]	McGARVEY J A, DENNY T P, SCHELL M A. Spatial-temporal and quantitative analysis of growth and EPSⅠ production by Ralstonia solanacearum in resistant and susceptible tomato cultivars[J]. Phytopathology, 1999, 89(12): 1233-1239. DOI: 10.1094/PHYTO.1999.89.12.1233 doi: 10.1094/PHYTO.1999.89.12.1233

[37]	王正荣,生吉萍,申琳.细菌胞外多糖的生物合成与基因控制[J].生物技术通报,2010(11):48-55. DOI:10.13560/j.cnki.biotech.bull.1985.2010.11.007 WANG Z R, SHENG J P, SHEN L. Biosynthesis of bacterial exopolysaccharides and gene cluster[J]. Biotechnology Bulletin, 2010(11): 48-55. (in Chinese with English abstract) doi: 10.13560/j.cnki.biotech.bull.1985.2010.11.007

[38]	PEYRAUD R, COTTRET L, MARMIESSE L, et al. A resource allocation trade-off between virulence and proliferation drives metabolic versatility in the plant pathogen Ralstonia solanacearum [J]. PLoS Pathogens, 2016, 12(10): e1005939. DOI: 10.1371/journal.ppat.1005939 doi: 10.1371/journal.ppat.1005939

[1]	MA Xiaokui1, JIANG Hua1, LI Junzhi2, CHEN Yong3，HE Xiaojing1. Optimization of medium components for the production of exopolysaccharide by Lycoperdon pyriforme Schaeff.: Pers. using response surface methodology*[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(5): 481-488.

[2]	DAI Xian-jun,ZHANG Wei,LIU Ming-qi，HUANG Xiao-lin. Isolation,identification and biological characteristics of an exopolysaccharideproducing lactic acid bacteria strain from intestinal tract of black porgy (Sparus macrocephalus)[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2011, 37(2): 149-154.

[3]	YE Ming,CHEN Wu-xi,PENG Wei,MA Jian,YANG Liu. Effects of batch and fed-batch cultures on biomass and exopolysaccharide production of Lachnum calyculiforme[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2010, 36(6): 630-634.

Viewed

Full text

Abstract

Cited

Shared

Discussed