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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2011, Vol. 37 Issue (2): 149-154    DOI: 10.3785/j.issn.1008-9209.2011.02.005
Biological sciences & biotechnology     
Isolation,identification and biological characteristics of an exopolysaccharideproducing lactic acid bacteria strain from intestinal tract of black porgy (Sparus macrocephalus)
DAI Xian-jun1,ZHANG Wei1,LIU Ming-qi1，HUANG Xiao-lin2
1 .College of Life Sciences , China Jiliang University , Hangzhou 310018 ,China ;2 .Zhejiang Mariculture Research Institute , Wenzhou , Zhejiang 325005 ,China
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Abstract  A strain of exopolysaccharide -producing lactic acid bacteria was isolated from intestines of blackporgy, Sparus macrocephalus ,and was identified as Leuconostoc lactis by conventional biochemical characteristics, 16S rDNA genes equence analysis.Physiological -biochemical tests for the isolate showed that it had good tolerance to acid, bilesalt and protease,but couldn,t live above 70 ℃ .The culture supernatant of  the isolate could inhibit the growth of Vibrio parahaemolyticus , Vibrio harveyi and Vibrio alginolyticus , and the inhibition zone diameters were 1.12‐1.22cm and had not significant difference( P ＞0.05) at different culture time of 24 , 32 ,40, 48 , 56 h. The concentrations of exopolysaccharidesin the culture medium were 167.24-184.45 mg.L －１ with no significant difference ( P＞ 0.05) at the culture time of 24 ,32, 40 , 48 , 56h. The erythrocyte and leukocyte counts, hemoglobin concentration and lymphocyte ratio of  black porgies increased significantly ( P＜ 0.05) after being fed orally exopolysaccharides from the isolate culture for 20d.The results above indicate the isolate, Leuconostoc lactis can be a candidate probiotic strain in marine aquaculture.

Published: 25 March 2011
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DAI Xian-jun
ZHANG Wei
LIU Ming-qi
HUANG Xiao-lin
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DAI Xian-jun,ZHANG Wei,LIU Ming-qi，HUANG Xiao-lin. Isolation,identification and biological characteristics of an exopolysaccharideproducing lactic acid bacteria strain from intestinal tract of black porgy (Sparus macrocephalus). Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(2): 149-154.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.02.005     OR     http://www.zjujournals.com/agr/Y2011/V37/I2/149


产胞外多糖的黒鲷肠道乳酸菌的分离鉴定及其生物学特性

通过常规鉴定和16S rDNA 序列分析 , 从黒鲷 ( Sparus macrocephalus ) 肠道中分离鉴定出1株明串珠菌属 ( Leuconostoc ) 乳酸菌菌株 ; 该分离株对酸、胆盐、蛋白酶具有良好耐受性 , 但对温度耐受能力较差 ; 分离株培养上清液对哈维氏弧菌 ( Vibrio harveyi ) 、溶藻弧菌 ( Vibrio alginolyticus ) 、副溶血弧菌( Vibrio parahaemolyticus ) 的抑菌圈直径为1.12 ～ 1.22cm, 不同培养时间的抑菌效果差异无统计学意义 ( P＞ 0.05) ; 分离株在培养24 、32 、40 、48 和56h时的胞外多糖质量浓度为167.24～ 184.45 mg.L －１ ,不同取样时间的胞外多糖质量浓度差异无统计学意义 ( P＞0.05) ; 灌服胞外多糖20d 后黑鲷血液的红细胞、白细胞数量和血红素浓度、淋巴细胞比率均显著升高 ( P＜0.05) . 综上 , 该分离株为产胞外多糖的明串珠属乳酸菌 , 可作为开发海水鱼用微生态制剂的候选菌株.
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