Study on influencing factors of bulblet enlargement <i>in vitro</i> of <i>Lilium amoenum</i>

doi:10.3785/j.issn.1008-9209.2023.03.161

Journal of Zhejiang University (Agriculture and Life Sciences)

2024, Vol. 50

Issue (1): 42-52 DOI: 10.3785/j.issn.1008-9209.2023.03.161

Horticultural Sciences

Study on influencing factors of bulblet enlargement in vitro of Lilium amoenum

Minhui ZHANG¹(

),Junji CHEN¹,Wei QUAN²,Zhijia GU²,Hongzhi WU¹(

)

^1.College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, Yunnan, China
^2.Yunnan Rural Science and Technology Service Center, Kunming 650021, Yunnan, China

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Abstract

Lilium amoenum is a rare lily germplasm with an agreeable rose fragrance. This study aimed to explore the optimal culture conditions and medium formula for the enlargement of L. amoenum bulblets in vitro. Bulblets of L. amoenum with a diameter of 0.4-0.6 cm were inoculated on Murashige & Skoog (MS) medium supplemented with different concentrations of sucrose or sucrose combined with naphthalene acetic acid (NAA), N-(phenylmethyl)-9H-purin-6-amine (6-BA), indole butyric acid (IBA), or paclobutrazol (PP₃₃₃). The culture conditions of 16 hours of light/8 hours of darkness and full darkness were set up. After 45 days of culture, the enlargement coefficient and diameter increase multiple of the bulblets were counted. The results showed that in the range of experimental concentrations, with the increase of sucrose and hormone concentrations, the enlargement coefficient and diameter increase multiples of L. amoenum bulblets overall first increased and then decreased. When the sucrose concentration was 50-120 g/L, the bulblet enlargement effect at 90 g/L sucrose was the greatest, and the bulblets were green and shiny, and had good cohesion. When 50-120 g/L sucrose was combined with 0.1-0.7 mg/L NAA/6-BA, 0.5-4.0 mg/L IBA or 5-40 mg/L PP₃₃₃, the effect of bulblet enlargement was the greatest at 70 g/L sucrose and 1.0 mg/L IBA, and the enlargement coefficient and diameter increase multiple were 3.63 and 1.83, respectively. The synergistic effect of high concentrations of sucrose and PP₃₃₃ significantly inhibited the enlargement of bulblets. Lilium amoenum bulblets cultured under 16 hours of light/8 hours of darkness grew better than those cultured under full darkness, but the rooting effect was greater under full darkness. This study provides a scientific basis for shortening bulb growth time in the field and for germplasm resource protection.

Key words： Lilium amoenum bulblets enlargement in vitro rapid propagation system

Received: 16 March 2023 Published: 01 March 2024

CLC:

S682.29

Corresponding Authors: Hongzhi WU E-mail: zmh97964@163.com;hwu1128@163.com

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	Minhui ZHANG
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Cite this article:

Minhui ZHANG,Junji CHEN,Wei QUAN,Zhijia GU,Hongzhi WU. Study on influencing factors of bulblet enlargement in vitro of Lilium amoenum. Journal of Zhejiang University (Agriculture and Life Sciences), 2024, 50(1): 42-52.

URL:

https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2023.03.161 OR https://www.zjujournals.com/agr/Y2024/V50/I1/42

玫红百合组培小鳞茎瓶内膨大影响因子的研究

玫红百合（Lilium amoenum）是具有玫瑰甜香味的珍稀百合资源。为探索玫红百合组培小鳞茎瓶内膨大的最适培养条件及培养基配方，以直径0.4～0.6 cm的玫红百合组培小鳞茎为材料，将其接种于添加不同质量浓度蔗糖或蔗糖与萘乙酸（naphthalene acetic acid, NAA）、6-苄氨基嘌呤[N-(phenylmethyl)-9H-purin-6-amine, 6-BA]、吲哚丁酸（indole butyric acid, IBA）、多效唑（paclobutrazol, PP₃₃₃）组合的Murashige & Skoog（MS）培养基中，设置16 h光照/8 h黑暗和全黑暗2种光照环境，培养45 d后统计小鳞茎的膨大系数和直径增大倍数。结果表明，在本试验条件下，随着蔗糖及激素质量浓度的升高，玫红百合组培小鳞茎膨大系数及直径增大倍数基本呈先增加后下降的趋势，其中：在添加蔗糖质量浓度为50～120 g/L处理中，以90 g/L蔗糖处理的膨大效果最好，小鳞茎嫩绿有光泽，抱合度好；当50～120 g/L蔗糖与0.1～0.7 mg/L NAA/6-BA、0.5～4.0 mg/L IBA或5～40 mg/L PP₃₃₃协同作用时，以70 g/L蔗糖和1.0 mg/L IBA处理的小鳞茎膨大效果最好，其膨大系数与直径增大倍数最高，分别为3.63和1.83；高质量浓度的蔗糖与PP₃₃₃协同作用会显著抑制小鳞茎的膨大。在16 h光照/8 h黑暗环境下培养的玫红百合组培小鳞茎较全黑暗环境下长势好，其膨大系数及直径增大倍数高，但黑暗环境下生根效果更好。本研究可缩短玫红百合田间培育成球时间，为种质资源保护提供了科学依据。

关键词： 玫红百合, 小鳞茎, 瓶内膨大, 快繁体系

Table 1 Sucrose and hormone treatments for bulblet enlargement in vitro of L. amoenum

Table 2 Combinations of sucrose and hormone for bulblet enlargement in vitro of L. amoenum

Fig. 1 Growth of L. amoenum bulblets treated with different sucrose concentrations for 45 dA-D. Sucrose concentration is 50, 70, 90, 120 g/L, respectively.

Fig. 2 Changes of enlargement coefficients of L. amoenum bulblets with different sucrose and NAA concentrationsDifferent lowercase letters above bars indicate significant differences at the 0.05 probability level, and the same as below.

Fig. 3 Changes of diameter increase multiples of L. amoenum bulbletswith different sucrose and NAA concentrationsThe same lowercase letter above bars indicates no significant differences at the 0.05 probability level, and the same as below.

Fig. 4 Changes of enlargement coefficients of L. amoenum bulblets with different sucrose and 6-BA concentrations

Fig. 5 Changes of diameter increase multiples of L. amoenum bulblets with different sucrose and 6-BA concentrations

Fig. 6 Changes of enlargement coefficients of L. amoenum bulblets with different sucrose and IBA concentrations

Fig. 7 Changes of diameter increase multiples of L. amoenum bulblets with different sucrose and IBA concentrations

Fig. 8 Growth of L. amoenum bulblets under high-concentration sucrose and IBA treatmentsA. Plants cultured in the medium of MS+70 g/L sucrose+1.0 mg/L IBA for 25 d; B-D. Plants cultured in the medium of MS+120 g/L sucrose+4.0 mg/L IBA for about 15, 25, 35 d, respectively.

Fig. 9 Changes of enlargement coefficients of L. amoenum bulblets with different sucrose and PP₃₃₃ concentrations

Fig. 10 Changes of diameter increase multiples of L. amoenum bulbletswith different sucrose and PP₃₃₃ concentrations

Table 4 Effects of adding hormones to 70 g/L sucrose on enlargement coefficients and diameter increase multiples of L. amoenum bulblets

Table 5 Effects of light conditions on bulblet enlargements of L. amoenum

Fig. 11 Growth of L. amoenum bulblets under different light conditionsA. Bulblets cultured under the 16 hours of light/8 hours of darkness environment; B. Bulblets cultured under the full darkness environment; C. Rooting of bulblets cultured under the 16 hours of light/8 hours of darkness environment; D. Rooting of bulblets cultured under the full darkness environment.


[1]	中国科学院中国植物志编辑委员会.中国植物志:第十四卷[M].北京:科学出版社,1980:116-166. Chinese Academy of Sciences. Editorial Committee of Flora of China. Flora of China: Vol. 14[M]. Beijing: Science Press, 1980: 116-166. (in Chinese)

[2]	LIANG S Y, TAMURA M N. Lilium Linnaeus Sp. Pl. 1: 302. 1753[M]//WU Z Y, RAVEN P H. Flora of China: Vol. 14. Beijing: Science Press, 2000: 118-152. (in English)

[3]	DU Y P, HE H B, WANG Z X, et al. Investigation and evaluation of the genus Lilium resources native to China[J]. Genetic Resources and Crop Evolution, 2014, 61(2): 395-412. DOI: 10.1007/s10722-013-0045-6 doi: 10.1007/s10722-013-0045-6

[4]	张雪敏,李佳敏,康圣楠,等.百合鳞茎发生发育研究进展[J/OL].分子植物育种,2022. ZHANG X M, LI J M, KANG S N, et al. Research progress on the development of lily bulbs[J/OL]. Molecular Plant Breeding, 2022. (in Chinese with English abstract)

[5]	吴丽芳,崔光芬,张艺萍,等.玫红百合高效离体繁殖和试管鳞茎诱导[J].植物生理学通讯,2010,46(9):967-968. DOI:10.13592/j.cnki.ppj.2010.09.021 WU L F, CUI G F, ZHANG Y P, et al. Efficient in vitro propagation and bulb induction of Lilium amoenum Wilson ex Sealy[J]. Plant Physiology Communications, 2010, 46(9): 967-968. (in Chinese with English abstract) doi: 10.13592/j.cnki.ppj.2010.09.021

[6]	陈姝男.宝兴百合组织培养和试管鳞茎膨大研究[D].成都:四川农业大学,2018. CHEN S N. Study on tissue culture and development of tube bulbls in Lilium duchartrei [D]. Chengdu: Sichuan Agricultural University, 2018. (in Chinese with English abstract)

[7]	任君芳,张利,刘建霞,等.东方百合‘Vivian’高效离体快繁体系的建立[J].四川林业科技,2020,41(6):84-88. DOI:10.12172/202009010001 REN J F, ZHANG L, LIU J X, et al. Establishment of an efficient approach in vitro propagation for oriental hybrid lily cultivar ‘Vivian’[J]. Journal of Sichuan Forestry Science and Technology, 2020, 41(6): 84-88. (in Chinese with English abstract) doi: 10.12172/202009010001

[8]	张彦妮,李兆婷,张艳波,等.毛百合试管鳞茎形成和膨大的培养优化[J].江苏农业科学,2016,44(4):74-78. DOI:10.15889/j.issn.1002-1302.2016.04.019 ZHANG Y N, LI Z T, ZHANG Y B, et al. Culture optimization of bulblet formation and enlargement in vitro of Lilium dauricum [J]. Jiangsu Agricultural Sciences, 2016, 44(4): 74-78. (in Chinese) doi: 10.15889/j.issn.1002-1302.2016.04.019

[9]	吕翠竹,王有国,王立.绿花百合组培芽增殖与试管鳞茎膨大的影响因素研究[J].西南林业大学学报(自然科学),2020,40(1):38-45. DOI:10.11929/j.swfu.201903131 LÜ C Z, WANG Y G, WANG L. Study on the influencing factors of bud proliferation and bulblet swelling of Lilium fargesii in vitro [J]. Journal of Southwest Forestry University (Natural Sciences), 2020, 40(1): 38-45. (in Chinese with English abstract) doi: 10.11929/j.swfu.201903131

[10]	周玲云,高素萍,陈锋.蔗糖和光周期在泸定百合试管鳞茎膨大中的作用机制[J].浙江大学学报(农业与生命科学版),2016,42(4):435-441. DOI:10.3785/j.issn.1008-9209.2015.09.151 ZHOU L Y, GAO S P, CHEN F. Action mechanism of sucrose and photoperiod on bulblet enlargement of Lilium sargentiae [J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 42(4): 435-441. (in Chinese with English abstract) doi: 10.3785/j.issn.1008-9209.2015.09.151

[11]	常琳,杜方,王丽婷,等.金石和伯爵百合鳞茎诱导及膨大研究[J].山西农业科学,2018,46(8):1279-1281. DOI:10.3969/j.issn.1002-2481.2018.08.13 CHANG L, DU F, WANG L T, et al. Study on bulblet induction and enlargement of Golden Stone and Album lily[J]. Journal of Shanxi Agricultural Sciences, 2018, 46(8): 1279-1281. (in Chinese with English abstract) doi: 10.3969/j.issn.1002-2481.2018.08.13

[12]	舒宁.几种云南野生百合资源调查引种及离体繁殖体系研究[D].武汉:华中农业大学,2015. SHU N. Research on investigation, introduction and micro-propagation system of resources of Lilium in Yunnan Province[D]. Wuhan: Huazhong Agricultural University, 2015. (in Chinese with English abstract)

[13]	王爱勤,周歧伟,何龙飞,等.百合试管结鳞茎的研究[J].广西农业大学学报,1998,17(1):71-75. WANG A Q, ZHOU Q W, HE L F, et al. Studies on the bulblet formation in tube of Lilium longiflorum L.[J]. Journal of Guangxi Agricultural University, 1998, 17(1): 71-75. (in Chinese with English abstract)

[14]	牛慧婷,杨道兰,冯炜弘,等.不同因子对兰州百合组培小鳞茎诱导及膨大的影响研究[J].甘肃农业科技,2022,53(6):66-71. DOI:10.3969/j.issn.1001-1463.2022.06.016 NIU H T, YANG D L, FENG W H, et al. Effects of different factors on bulblet induction and enlargement in Lanzhou lily bulb tissue cultivation[J]. Gansu Agricultural Science and Technology, 2022, 53(6): 66-71. (in Chinese with English abstract) doi: 10.3969/j.issn.1001-1463.2022.06.016

[15]	任亚萍.百合试管鳞茎形成及膨大的研究[D].武汉:华中农业大学,2008. REN Y P. Studies on formation and enlargement of lily (Lilium L.) bulblets in vitro[D].Wuhan: Huazhong Agricultural University, 2008. (in Chinese with English abstract)

[16]	周玲云.蔗糖在泸定百合试管鳞茎形成及膨大过程中的信号作用初探[D].成都:四川农业大学,2016． ZHOU L Y. Study of signal effects of sucrose on formation and enlargement of bulblet of Lilium sargentiae [D]. Chengdu: Sichuan Agricultural University, 2016. (in Chinese with English abstract)

[17]	裴怀弟,李琦,李淑洁,等.兰州百合试管小鳞茎膨大及碳水化合物变化规律研究[J].甘肃农业科技,2021,52(12):5-10. DOI:10.3969/j.issn.1001-1463.2021.12.002 PEI H D, LI Q, LI S J, et al. Regularity of enlargement and carbohydrate changes in bulblets in vitro of Lanzhou lily[J]. Gansu Agricultural Science and Technology, 2021, 52(12): 5-10. (in Chinese with English abstract) doi: 10.3969/j.issn.1001-1463.2021.12.002

[18]	张延龙,梁建丽,牛立新.东方百合试管鳞茎膨大的研究[J].西北农林科技大学学报(自然科学版),2006,34(6):75-78. DOI:10.13207/j.cnki.jnwafu.2006.06.015 ZHANG Y L, LIANG J L, NIU L X. Research on the growth of oriental lily bulblet in tube[J]. Journal of Northwest Sci-Tech University of Agriculture and Forestry (Natural Science Edition), 2006, 34(6): 75-78. (in Chinese with English abstract) doi: 10.13207/j.cnki.jnwafu.2006.06.015

[19]	孙玉燕,李锡香.蔬菜变态根茎发育的分子机制研究进展[J].中国农业科学,2015,48(6):1162-1176. DOI:10.3864/j.issn.0578-1752.2015.06.12 SUN Y Y, LI X X. A review on molecular mechanism of the modified roots or stems development in vegetables[J]. Scientia Agricultura Sinica, 2015, 48(6): 1162-1176. (in Chinese with English abstract) doi: 10.3864/j.issn.0578-1752.2015.06.12

[20]	王丽婷.百合组培苗鳞茎诱导及膨大研究[D].晋中:山西农业大学,2017. WANG L T. Study on bulb induction and expansion of lily by tissue culture[D]. Jinzhong: Shanxi Agricultural University, 2017. (in Chinese with English abstract)

[21]	张艳波.毛百合组织培养与试管鳞茎膨大的研究[D].哈尔滨:东北林业大学,2013. ZHANG Y B. Study on tissue culture and bulblet swelling of Lilium dauricum [D]. Harbin: Northeast Forestry University, 2013. (in Chinese with English abstract)

[22]	朱岩,王红,代宁,等.大花卷丹百合组培快繁技术研究[J].甘肃农业科技,2022,53(9):76-80. DOI:10.3969/j.issn.1001-1463.2022.09.018 ZHU Y, WANG H, DAI N, et al. Study on techniques of rapid propagation for the tissue culture of Lilium leichtlinii var. maximowiczii (Regel) Baker[J]. Gansu Agricultural Science and Technology, 2022, 53(9): 76-80. (in Chinese with English abstract) doi: 10.3969/j.issn.1001-1463.2022.09.018

[23]	吴银亮,王红霞,杨俊,等.甘薯储藏根形成及其调控机制研究进展[J].植物生理学报,2017,53(5):749-757. DOI:10.13592/j.cnki.ppj.2017.1014 WU Y L, WANG H X, YANG J, et al. Advances in storage root development and regulation in sweetpotato [Ipomoea batatas (L.) Lam.][J]. Plant Physiology Journal, 2017, 53(5): 749-757. (in Chinese with English abstract) doi: 10.13592/j.cnki.ppj.2017.1014

[24]	梁艳,杨晓杰,陈典,等.多效唑对大蒜试管微鳞茎形成和膨大的影响[J].北方园艺,2010(5):57-59. LIANG Y, YANG X J, CHEN D, et al. Effects of PP333 concentration on the bulblet formation and growth of ‘Acheng’ garlic in vitro [J]. Northern Horticulture, 2010(5): 57-59. (in Chinese with English abstract)

[25]	王晓丽,韩立群,刘杰,等.活性炭和多效唑对垂花百合试管鳞茎膨大的影响[J].安徽农业科学,2011,39(19):11429-11430. DOI:10.13989/j.cnki.0517-6611.2011.19.222 WANG X L, HAN L Q, LIU J, et al. Effects of AC and PP333 on the development of the tube bulbs of Lilium cernuum [J]. Journal of Anhui Agricultural Sciences, 2011, 39(19): 11429-11430. (in Chinese with English abstract) doi: 10.13989/j.cnki.0517-6611.2011.19.222

[26]	王晓丽.垂花百合组织培养及试管鳞茎膨大初步研究[D].哈尔滨:东北林业大学,2011. WANG X L. Study on tissue culture and development of tube bulbs in Lilium cernuum [D].Harbin: Northeast Forestry University, 2011. (in Chinese with English abstract)

[27]	孙红梅,张静,李雪艳,等.光暗不同培养条件下植物生长调节剂对亚洲百合鳞茎诱导的影响[J].沈阳农业大学学报,2011,42(5):549-554. DOI:10.3969/j.issn.1000-1700.2011.05.007 SUN H M, ZHANG J, LI X Y, et al. Lilium Asiatic hybrid ‘Elite’ bulblets formation in vitro: effects of plant growth regulators as well as culture condition[J]. Journal of Shenyang Agricultural University, 2011, 42(5): 549-554. (in Chinese with English abstract) doi: 10.3969/j.issn.1000-1700.2011.05.007

[28]	张延龙,张启翔,薛晓娜.光周期对野生卷丹试管苗鳞茎形成及糖代谢的影响[J].园艺学报,2010,37(6):957-962. DOI:10.16420/j.issn.0513-353x.2010.06.015 ZHANG Y L, ZHANG Q X, XUE X N. The effects of the photoperiods on the bulblet formation and sugar metabolism change of wild Lilium lancifolium in vitro [J]. Acta Horticulturae Sinica, 2010, 37(6): 957-962. (in Chinese with English abstract) doi: 10.16420/j.issn.0513-353x.2010.06.015

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