Characteristics of the rhizosphere bacterial community of endangered plant <i>Cupressus gigantea</i> in Tibet

doi:10.3785/j.issn.1008-9209.2022.03.071

Journal of Zhejiang University (Agriculture and Life Sciences)

2023, Vol. 49

Issue (2): 241-252 DOI: 10.3785/j.issn.1008-9209.2022.03.071

Resource utilization & environmental protection

Characteristics of the rhizosphere bacterial community of endangered plant Cupressus gigantea in Tibet

Wenfeng GONG¹(

),Zeying WANG²,Jinliang LIU³,Yu SUN¹,Xinxin YANG²,Shuai WEI²,Liping WEI²(

)

^1.College of Plant Sciences, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, Xizang, China
^2.College of Resources and Environment, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, Xizang, China
^3.College of Forestry, Northwest A & F University, Yangling 712100, Shaanxi, China

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Abstract

Rhizosphere microorganisms play an important role in plant growth and adaptation to the environment. In order to reveal the characteristics of rhizosphere bacterial community of endangered plant Cupressus gigantea in Tibet, we employed 16S rRNA gene high-throughput amplicon sequencing technology combined with soil chemical properties to examine the rhizosphere bacterial composition and diversity in Bayi District, Milin County, and Langxian County of Nyingchi City in Tibet and their influencing factors. The results showed that the diversity of bacteria in the rhizosphere of C. gigantea was rich, and the dominant bacterial phylum were Actinobacteriota, Proteobacteria, and Acidobacteriota. There were 757 shared genera (65.83%) of rhizosphere bacteria in C. gigantea in Langxian County, Bayi District, and Milin County, with 125 (10.87%), 39 (3.39%) and 41 (3.56%) unique genera, respectively, and the β-diversity analysis of non-metric multidimensional scaling based on Bray-Curtis distance revealed significant differences among populations. The positive correlation of the co-occurrence network of bacterial communities was more than 68%, and the cooperative relationship was greater than the competitive relationship. Soil available phosphorus (AP), total phosphorus (TP), and ammonium nitrogen (NH₄⁺-N) were important factors driving changes in the structure of rhizosphere bacterial community. In order to adapt to different growth environments, various groups of C. gigantea have formed unique rhizosphere bacterial communities. The above results can provide a reference for studying the role of rhizosphere microorganisms in the growth and environmental adaptation of C. gigantea and the protection of C. gigantea resources.

Key words： Cupressus gigantea rhizosphere bacteria community structure soil chemical characteristics

Received: 07 March 2022 Published: 25 April 2023

CLC:

Q938.1

Corresponding Authors: Liping WEI E-mail: zkxygwf@xza.edu.cn;34984262@qq.com

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Cite this article:

Wenfeng GONG,Zeying WANG,Jinliang LIU,Yu SUN,Xinxin YANG,Shuai WEI,Liping WEI. Characteristics of the rhizosphere bacterial community of endangered plant Cupressus gigantea in Tibet. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(2): 241-252.

URL:

https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2022.03.071 OR https://www.zjujournals.com/agr/Y2023/V49/I2/241

西藏濒危植物巨柏根际细菌群落特征

根际微生物在植物生长和环境适应中起着重要的作用。为揭示西藏濒危珍稀植物巨柏根际细菌群落特征，通过16S rRNA基因扩增子高通量测序技术，并结合土壤化学特性，分析西藏林芝市巴宜区、米林县、朗县3个种群巨柏根际细菌的组成、多样性及影响因子。结果显示：巨柏根际细菌多样性丰富，其中优势菌门为放线菌门（Actinobacteriota）、变形菌门（Proteobacteria）和酸杆菌门（Acidobacteriota）。朗县、巴宜区和米林县巨柏根际细菌共有属为757个（65.83%），独有属分别为125个（10.87%）、39个（3.39%）和41个（3.56%）；基于Bray-Curtis距离的非度量多维尺度的β多样性分析显示，不同种群间根际细菌群落差异显著。细菌群落的共现性网络正相关连接均在68%以上，表明群落间合作关系大于竞争关系。土壤中有效磷、全磷、铵态氮是驱动根际细菌群落结构变化的重要因子。为适应不同生长环境，各种群巨柏形成了特有的根际细菌群落。本研究结果可为探究根际微生物在巨柏生长和环境适应中的作用及保护巨柏植物资源提供参考。

关键词： 巨柏, 根际细菌, 群落结构, 土壤化学特性

Fig. 1 Analysis of compositions and differences of rhizosphere bacteria in three populations of C. giganteaSingle asterisk (*) indicates significant differences at the 0.05 probability level.

采样点 Sampling site	编号 Serial number	纬度 Latitude (N)	经度 Longitude (E)	海拔 Altitude/m	胸径 Diameter at breast height/m	生境 Habitat	主要伴生种 Main associated species
巴宜区 Bayi District (BY)	1	29°37 $′$ 36.94 $″$	94°23 $′$ 57.73 $″$	3 105.00	0.12	高山峡谷半阳坡沙质土疏林地	绢毛蔷薇（Rosa sericea）、二色锦鸡儿（Caragana bicolor）、楔叶绣线菊（Spiraea canescens）等
	2	29°37 $′$ 36.22 $″$	94°23 $′$ 55.06 $″$	3 064.00	0.15
	3	29°37 $′$ 32.64 $″$	94°23 $′$ 59.75 $″$	3 068.00	0.24
	4	29°37 $′$ 30.69 $″$	94°24 $′$ 04.34 $″$	3 068.00	0.77
	5	29°37 $′$ 38.54 $″$	94°23 $′$ 56.18 $″$	3 128.00	1.27
	6	29°37 $′$ 23.66 $″$	94°24 $′$ 07.46 $″$	3 063.00	1.50
	7	29°37 $′$ 29.88 $″$	94°23 $′$ 29.19 $″$	3 003.00	1.78
	8	29°37 $′$ 38.51 $″$	94°23 $′$ 57.58 $″$	3 126.00	1.87
	9	29°37 $′$ 37.77 $″$	94°23 $′$ 57.51 $″$	3 116.00	2.44
朗县 Langxian County (LX)	1	28°59 $′$ 35.57 $″$	93°18 $′$ 23.40 $″$	3 030.00	0.10	高山峡谷阳坡沙质土疏林地	砂生槐（Sophora moorcroftiana）、西藏野丁香（Leptodermis xizangensis）、猪毛蒿（Artemisia scoparia）、木帚栒子（Cotoneaster dielsianus）、米林蔓黄芪（Phyllolobium milingense）、翅果蓼（Fagopyrum tibeticum）等
	2	28°59 $′$ 09.00 $″$	93°14 $′$ 00.67 $″$	3 002.00	0.13
	3	29°04 $′$ 03.84 $″$	93°04 $′$ 37.60 $″$	3 088.00	0.50
	4	29°04 $′$ 03.88 $″$	93°04 $′$ 36.50 $″$	3 068.00	0.57
	5	29°04 $′$ 02.69 $″$	93°04 $′$ 38.76 $″$	3 100.00	0.72
	6	28°59 $′$ 32.66 $″$	93°18 $′$ 23.12 $″$	3 076.00	0.98
	7	29°04 $′$ 02.37 $″$	93°04 $′$ 38.39 $″$	3 100.00	1.61
	8	29°00 $′$ 47.70 $″$	93°19 $′$ 00.88 $″$	3 014.00	2.25
	9	29°04 $′$ 19.88 $″$	93°23 $′$ 11.80 $″$	3 030.00	3.00
米林县 Milin County (ML)	1	29°07 $′$ 39.90 $″$	93°48 $′$ 49.27 $″$	3 063.00	0.20	高山峡谷阳坡沙质土疏林地	高山松（Pinus densata）、砂生槐（Sophora moorcroftiana）、西南野丁香（Leptodermis purdomii）、烦果小檗（Berberis ignorata ）、二色锦鸡儿（Caragana bicolor）、川藏香茶菜（Isodon pharicus）等
	2	29°05 $′$ 24.80 $″$	93°23 $′$ 59.60 $″$	3 115.00	0.37
	3	29°09 $′$ 04.36 $″$	93°27 $′$ 55.24 $″$	2 982.00	0.45
	4	29°08 $′$ 39.80 $″$	93°27 $′$ 45.00 $″$	3 057.00	0.87
	5	29°05 $′$ 24.80 $″$	93°23 $′$ 59.60 $″$	3 115.00	1.03
	6	29°08 $′$ 39.80 $″$	93°27 $′$ 45.00 $″$	3 057.00	1.38
	7	29°08 $′$ 39.80 $″$	93°27 $′$ 45.00 $″$	3 057.00	1.67
	8	29°07 $′$ 33.22 $″$	93°51 $′$ 11.80 $″$	3 046.00	2.06
	9	29°07 $′$ 32.79 $″$	93°51 $′$ 11.50 $″$	3 050.00	2.29

Table 1 Basic information of sampling sites

Table 2 Soil chemical characteristics in the rhizosphere of three populations of C. gigantea

Fig. 2 Venn diagram of rhizosphere bacteria in C. gigantea at the genus level

Table 3 Alpha diversity analysis of rhizosphere bacteria in C. gigantea

Fig. 3 β-diversity analysis of NMDS based on Bray-Curtis distance

Fig. 4 Analysis of rhizosphere bacterial network of three populations of C. giganteaA. Network diagram of rhizosphere bacteria in three populations of C. gigantea; B. Within-module connectivity (Z_i) and among-module connectivity (P_i) of rhizosphere bacterial nodes in three populations of C. gigantea.

Table 4 Characteristics of bacterial community network in the rhizosphere of C. gigantea

Fig. 5 Pearson correlation analysis between soil chemical factors and rhizosphere bacteria (Bacteriophyta) of C. giganteaSingle asterisk (*) indicates significant correlations at the 0.05 probability level; double asterisks (**) indicate extremely significant correlations at the 0.01 probability level. n=27.

Fig. 6 RDA of soil chemical factors and rhizosphere bacterial OTUs of C. giganteaThe red dots in the figure represent sample groups; the black arrows represent OTUs, and the red arrows indicate quantitative soil chemical factors, and the lengths of arrows represent the degree of impact (explanatory quantity) of soil chemical factors on rhizosphere bacterial community.

Table 5 RDA of soil chemical factors


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