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Journal of Zhejiang University (Agriculture and Life Sciences)  2021, Vol. 47 Issue (2): 203-211    DOI: 10.3785/j.issn.1008-9209.2020.07.291
Food sciences     
Aroma compositions and comparison of their related components of Longjing tea with different aroma types
Senjie ZHOU(),Chuangsheng HUANG,Chunlin LI,Shuying GONG,Haowei GUO,Yilin TONG,Fangyuan FAN()
Tea Research Institute, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
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Abstract  

Longjing tea is a famous traditional tea in China and the aroma is an important factor in the quality evaluation of tea. In this study, Longjing tea samples were widely collected and explored by sensory evaluation, which dividing them into three aroma groups: tender aroma type, clean and refreshing aroma type, and approaching high aroma type. Then, simultaneous distillation extraction (SDE) and gas chromatography-mass spectrometry (GC-MS) were used for extraction and determination of aroma compositions. The result suggested that the aroma-characteristic compositions of Longjing tea with tender aroma, clean and refreshing aroma, and approaching high aroma, respectively, showed differences in types and relative contents of alcohols, aldehydes, terpenes, ketones, alkanes, alkenes, heterocycles, esters and so on. The results of principal component analysis (PCA) based on main aroma components showed that: 1) The contents of styrene, cetane, 3-methoxy-1, 2-propanediol were significantly and positively correlated with the tender aroma of Longjing tea; 2) the contents of (E)-β-farnesene, trans-4-tert-butylcyclohexanol and methyl jasmonate were significantly and positively correlated with the clean and refreshing aroma of Longjing tea; 3) the relative contents of phenylacetonitrile, acetophenone, 1, 3-cyclohexadiene-2, 2, 6-trimethyl-benzaldehyde were higher in Longjing tea with approaching high aroma. This study provides a theoretical basis for aroma description in sensory evaluation of Longjing tea.



Key wordsLongjing tea      aroma performance      aroma component      principal component analysis     
Received: 29 July 2020      Published: 25 April 2021
CLC:  S 571.1  
Corresponding Authors: Fangyuan FAN     E-mail: zhousenjie1996@163.com;FangyFan@zju.edu.cn
Cite this article:

Senjie ZHOU,Chuangsheng HUANG,Chunlin LI,Shuying GONG,Haowei GUO,Yilin TONG,Fangyuan FAN. Aroma compositions and comparison of their related components of Longjing tea with different aroma types. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(2): 203-211.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2020.07.291     OR     http://www.zjujournals.com/agr/Y2021/V47/I2/203


不同香气类型龙井茶香气组成及其相关组分比较

本研究广泛收集龙井茶样品,基于感官分析表现出的嫩香、清鲜、尚高特征的香气类型分组,利用可控温的同时蒸馏萃取法(simultaneous distillation extraction, SDE)提取其香气物质,并结合气相色谱-质谱联用(gas chromatography-mass spectrometry, GC-MS)技术进行香气组分及特征的比较。结果显示:嫩香类、清鲜类及尚高类龙井茶类型间的香气组分在醇类、醛类、萜烯类、酮类、烷烃类、烯类、杂环类及酯类等化合物上表现出种类及相对含量的差异性。基于主要香气组分进行主成分分析(principal component analysis, PCA)的结果显示:1)龙井茶嫩香特征与香气组分中的苯乙烯、十六烷、3-甲氧基-1,2-丙二醇等化合物含量呈明显的正相关性;2)清鲜特征与(E)-β-金合欢烯、反-4-叔丁基环己醇、甲基茉莉酮酸酯含量呈明显的正相关性;3)尚高类龙井茶样品中的苯乙腈、苯乙酮、1,3-环己二烯-2,2,6-三甲基苯甲醛等组分含量较高。本研究可为龙井茶感官审评中的香气描述提供理论依据。


关键词: 龙井茶,  香气特征,  香气组分,  主成分分析 

编号

No.

产地

Place of production

等级

Grade

品种

Variety

NX1绍兴新昌特级乌牛早
NX2磐安玉峰特级龙井群体种
NX3杭州翁家山一级龙井43
NX4杭州钱塘一级龙井群体种
NX5杭州淳安特级龙井43
NX6绍兴新昌特级乌牛早
NX7杭州西湖特级龙井43
NX8杭州富阳特级混合品种
QX1杭州余杭特级混合品种
QX2杭州淳安一级龙井群体种
QX3绍兴新昌一级乌牛早
QX4绍兴新昌特级乌牛早
QX5杭州西湖一级龙井43
QX6杭州富阳特级混合品种
SG1杭州富阳四级混合品种
SG2杭州淳安四级混合品种
SG3绍兴新昌三级龙井43
SG4绍兴嵊州二级龙井43
SG5磐安玉峰四级混合品种
SG6杭州余杭三级龙井43
SG7绍兴新昌四级龙井43
SG8杭州富阳四级龙井43
Table 1 Information of the tested tea samples
编号No.

感官评价

Sensory evaluation

评分

Scoring

香气

类型

Aroma

type

外形

Appearance

汤色

Liquor color

香气

Aroma

滋味

Taste

叶底

Brewed leaves

香气

Aroma

总分

Total

score

NX1

扁平,光滑,挺直,尖削,匀

整,带鱼叶,较嫩绿,油润

较嫩绿,明亮嫩鲜

较醇厚,鲜

爽,微涩

幼嫩厚实,成朵,带鱼

叶,较嫩绿,较明亮

94.0093.70嫩香类
NX2扁平,光滑,挺直,绿翠嫩绿,清澈明亮嫩香,鲜爽醇厚,较甘

较嫩厚,多芽,成朵,

尚嫩绿,明亮

92.0092.95
NX3

扁平,挺直,光滑,尖削,嫩

绿,油润

嫩绿,清澈明亮

嫩香,馥郁,

鲜爽,有栗香

甘醇,鲜爽

嫩厚,成朵,较嫩绿,

明亮

95.0094.98
NX4

扁平,光滑,挺直,尖削,嫩

绿,较鲜活,油润,匀净

嫩绿,清澈明亮

嫩香,馥郁,

鲜爽

醇厚,甘鲜,

较浓

幼嫩厚实,多芽,成

朵,匀齐,嫩绿,鲜亮

95.0094.75
NX5

扁平,挺直,光滑,尖削,带

鱼叶,稍宽,嫩绿,尚油润

较嫩绿,明亮嫩香,闷甜尚醇厚,甘鲜

嫩厚,成朵,尚嫩绿,

尚明亮

92.0093.45
NX6

扁平,光滑,挺直,尖削,匀

整,带鱼叶,嫩绿,较鲜润

嫩绿,清澈明亮嫩香显,略有花香(越冬芽)甘醇,鲜爽

幼嫩厚实,成朵,带

鱼叶,嫩绿,鲜亮

94.0094.80
NX7

扁平,挺直,光削,匀整,嫩

绿,较鲜润

嫩绿,明亮

嫩香显(越

冬芽)

甘醇,较爽

(欠鲜)

细嫩厚实,嫩绿,明亮92.0093.48
NX8扁平,光滑,挺直,绿嫩绿,清澈明亮嫩香,略陈

尚浓醇,清

鲜,尚甘润

嫩厚,多芽,成朵,较

匀齐,较嫩绿,明亮

95.0093.80
QX1

扁平,光滑,挺直,尚尖削,嫩

绿,鲜活带翠

尚杏绿,明亮

清鲜,有嫩

香,略带花香

较醇厚,鲜爽

较细嫩,成朵,匀齐,

尚嫩绿,明亮

94.0093.05清鲜类
QX2

扁平,挺直,光削,较匀整,

嫩绿,油润

嫩绿,清澈明亮清鲜,花香显尚浓醇,清鲜细嫩厚实,嫩绿,明亮93.0094.27
QX3

扁平,挺直,尖削,匀整,嫩

绿,油润

较嫩绿,明亮清高,鲜爽

尚浓醇,青

尚爽

细嫩,成朵,嫩绿, 明亮94.0093.20
QX4

扁平,挺直,光削,嫩绿显

翠,光润

较嫩绿,明亮

清高,鲜爽,

略有花香

尚浓醇,较鲜幼嫩厚实,嫩绿,明亮93.5093.48
QX5

扁平,光滑,挺直,尖削,嫩

绿,较鲜活,油润,匀净

嫩绿,清澈明亮清香,鲜爽醇厚,甘鲜

幼嫩厚实,多芽,成

朵,匀齐,嫩绿,鲜亮

95.0094.75
QX6

扁平,挺直,光削,匀整,绿

润,略带青条

嫩绿,明亮

清鲜,有花

香,微生

较醇厚,清

爽,生涩

细嫩厚实,嫩绿,明亮94.5093.69
SG1

扁平,尚挺直,多青条、暗条,

深绿,稍花杂

尚嫩绿,明亮尚高,纯正

浓,青涩,

微苦

嫩,有条,黄绿,较明亮84.0079.95尚高类
SG2

扁平,尚挺直,稍宽松,多青

壳,稍黄绿

嫩绿,明亮尚高,火工足醇,尚爽厚软,有芽,较绿亮83.0081.10
SG3

扁平,较挺直,尚光,较宽松,

多片,有青壳、青条,尚绿翠

黄较亮

尚高,生,

微闷

浓,生涩,苦嫩有芽,多单张,绿亮82.0080.90
SG4

扁平,尚挺直,尚光,多青

条、紧条,绿

黄亮平和,尚高尚浓,微涩多嫩单张,绿尚亮85.0082.75
SG5扁平,松空(青壳),青枯绿亮

尚高,略带

花香

醇和,清爽,

有花香

软尚厚,绿亮82.0078.50
SG6扁欠平,欠挺直,黄绿稍暗黄亮

尚高,略老

火,微闷

较浓,略涩,

糙,闷,老火

尚嫩,多摊张,有红

茎,黄绿,稍暗

83.0078.48
SG7扁平,尚挺直,宽松,绿尚润杏绿,尚亮尚高

浓,涩,略苦,

微生

尚嫩,多单张,有芽,

绿亮

84.0081.43
SG8

扁平,尚挺直,稍狭长,深

绿,稍花杂

尚嫩绿,明亮尚高,有花香较浓,微苦涩

尚嫩,略有芽,多青

张,尚绿亮

82.0077.85
Table 2 Sensory evaluation and scoring of the tested tea samples
Fig. 1 Aroma components of Longjing tea with different aroma types
Fig. 2 Heat map of contents of aroma components in Longjing tea samples with different aroma types
Fig. 3 Distribution of tea samples and score chart of chemical components in Longjing tea based on PCAIn figure B, please see the Fig. 2 for the details of numbers.
Fig. 4 PCA chart of main aroma compounds and correlation chart between chemical components and aroma properties

香气物质

Aroma compound

化学式

Chemical formula

香气特征

Aroma performance

苯乙烯 StyreneC8H8油脂类香气*
十六烷 CetaneC16H34烃类气味*
3-甲氧基-1,2-丙二醇 3-methoxy-1, 2-propanediolC4H10O3类似甜杏、坚果类香气*
甲基茉莉酮酸酯 Methyl jasmonateC13H20O3茉莉花香气[14]
1,1,6-三甲基-1,2-二氢萘 1, 1, 6-trimethyl-1, 2-dihydronaphthaleneC13H16甘草香气[20]
二氢猕猴桃内酯 DihydroactinidideC11H16O2玫瑰花香气[19]
2,3-二氢苯并呋喃 2, 3-dihydrobenzofuranC8H8O芳香气味*
香豆素 CoumarinC9H6O2甜草香气[21]
乙烯基-2-甲氧基苯酚 Vinyl-2-methoxyphenolC9H10O2辛辣、烟熏气味[5]
E)-β-金合欢烯 (E)-β-farneseneC15H24花香、甜香[22]
反-4-叔丁基环己醇 trans-4-tert-butylcyclohexanolC10H20O类似广藿香的香气*
苯乙腈 PhenylacetonitrileC8H7N刺激性气味*
苯乙酮 AcetophenoneC8H8O水果香气*
Table 3 Performances of main related aroma compounds
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[3] XU Pengcheng, FAN Fangyuan, LU Debiao, JIN jing, GONG Shuying1. Infusion color and taste characteristic of Dafo Longjing tea processed from different cultivars[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2017, 43(3): 317-328.
[4] ZHAI Rongrong, YU Peng, YE Shenghai, WANG Junmei, WU Mingguo, LIN Jianrong, ZHU Guofu, ZHANG Xiaoming. Screening and comprehensive evaluation of low nitrogen tolerance of Zhejiang photosensitive japonica rice cultivars[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 42(5): 565-.
[5] Su Wenhao, Liu Guishan*, He Jianguo, Wang Songlei, He Xiaoguang, Wang Wei, Wu Longguo . Detection of external defects on potatoes by hyperspectral imaging technology and image processing method.[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2014, 40(2): 188-196.
[6] ZHU Zuochun, TANG Yi*, PAN Rong, HU Shuqin. Effect of heating rate on the quality of Longjing tea during baking and aromaimproving process[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(4): 435-443.
[7] WU Yifei1, YAO Xiaohong1, SUN Hong2, WANG Xin1, TANG Jiangwu1. Optimization of solidstate fermentation conditions of rapeseed meal using response surface analysis combined with principal component analysis[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2012, 38(4): 490-496.
[8] FENG Lei,ZHANG Derong, CHEN Shuangshuang,FENG Bin, XIE Chuanqi,CHEN Youyuan,HE Yong. Early detection of gray mold on eggplant leaves using hyperspectral imaging technique.[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2012, 38(3): 311-317.
[9] TANG Jiang-wu,SUN Hong,YAO Xiao-hong,WU Yi-fei,WANG Xin. Fate,dissemination of Bacillus subtilis spores in gastrointestinal tract of broiler chicken and its effect onintestinal flora and digestive enzyme activities[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2011, 37(3): 319-325.
[10] ZOU Wei,FANG Hui,ZHOU Kang-yun,BAO Yi-dan,HE Yong. Identification of rapeseed varieties based on hyperspectral imagery[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2011, 37(2): 175-180.
[11] . Phenotypic variation and relationships among sesame (Sesamum indicum L.) sub-core collections[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2004, 30(1): 10-16.