|
|
Effects of different storage time on polysaccharide content and antioxidant activity of edible fungi |
YANG Hui, KE Leqin, SHU Ruonan, CHEN Yuwei, ZHU Tingyu, WANG Jun, FAN Jintao, WANG Liangliang* |
College of Ecology, Lishui University, Lishui 323000, Zhejiang, China |
|
|
Abstract
The Lentinula edodes 808, Pleurotus eryngii kE015 and Auricularia auricula-judae 916 were used as materials to investigate the effects of different storage time on polysaccharide content and its protective effect on HepG2 cells under oxidative stress. The results showed that the extraction rate of Lentinula edodes 808 was significantly higher than that of the other two kinds of edible fungi, and the yield of polysaccharide in Lentinula edodes 808, stored for three years was the highest with (6.92±0.18)% . However, the antioxidant effect of Lentinula edodes 808 stored for one year was better than the other years. The polysaccharides extracted from Pleurotus eryngii kE015 stored for two or three years had a better effect on the survival rate of HepG2 cells after oxidative injury than the other storage time. The highest survival rate of HepG2 cells reached (99.37±8.23)% at the polysaccharide concentration of 1 mg/mL and storage for three years, which was 1.91 times of the control group, indicating that the polysaccharide extracted from Pleurotus eryngii kE015 could effectively reduce the H2O2-induced oxidative damage of HepG2 cells. Consistently, the polysaccharide extracted from Pleurotus eryngii kE015 significantly increased the superoxide dismutase (SOD) activity, and reduced malondialdehyde (MDA) level in HepG2 cells after oxidative injury. In the Auricularia auricula-judae 916, one-year storage had the highest polysaccharide yield, while the four-year storage displayed the highest antioxidant activity. This study provides some references for optimizing storage time and conditions, and obtaining better production process in production of edible fungi such as Lentinula edodes, Pleurotus eryngii and Auricularia auricula-judae.
|
Published: 29 June 2018
|
|
不同贮藏时间对食用菌多糖含量及其抗氧化活性的影响
以香菇808、杏鲍菇kE015和黑木耳916为供试材料,探讨不同贮藏时间对食用菌多糖提取率及其对HepG2细胞氧化损伤的保护作用。结果表明:3种食用菌中香菇多糖的提取率明显高于其他2种,且贮藏3年的香菇多糖提取率最高,为(6.92±0.18)%;而贮藏1年的香菇多糖抗氧化效果优于其他年限。在贮藏2年和3年的杏鲍菇多糖处理组中,经H2O2氧化损伤后的HepG2细胞存活率优于其他2个年限,且以贮藏时间为3年、多糖质量浓度为1 mg/mL时HepG2细胞存活率最高,达(99.37±8.23%,为对照组的1.91倍,表明杏鲍菇多糖可以有效降低对H2O2诱导的HepG2细胞的氧化损伤作用;相应地,杏鲍菇多糖还显著提高了氧化损伤后HepG2细胞的超氧化物歧化酶活性,降低了丙二醛含量。在黑木耳组中贮藏1年的多糖提取率最高,贮藏4年的抗氧化活性最好。本研究为香菇、杏鲍菇、黑木耳等食用菌在生产实践中优化贮藏时间及条件、获得良好的生产工艺提供了一定的参考。
关键词:
食用菌,
贮藏时间,
多糖,
抗肿瘤活性,
抗氧化活性
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|