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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2010, Vol. 36 Issue (6): 630-634    DOI: 10.3785/j.issn.1008-9209.2010.06.006
Biological sciences & biotechnology     
Effects of batch and fed-batch cultures on biomass and exopolysaccharide production of Lachnum calyculiforme
YE Ming,CHEN Wu-xi,PENG Wei,MA Jian,YANG Liu
YE Ming,College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009,China CHEN Wu-xi,College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009,China PENG Wei,College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009,China MA Jian,College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009,China YANG Liu,College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009,China
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Abstract  The effects of cultural conditions on the biomass and exopolysaccharide (EPS) production of Lachnumcalyculiforme were studied by shake flask experiments .Batch and fed-batch cultures were held in5 L stirred fermentor,and the effects of the two culture methods on the biomass and EPS production of L . calyculiforme were compared . At 25℃,shake flask experiments indicated that the optimum conditions for biomass and EPS production of L . calyculiforme were as follows : glucose 30 g.L-1,yeast extract5 g.L-1 and the pH 6.0 . The 10 g.L-1 olive oil significantly stimulated the mycelial growth,and 10 g.L-1 soybean oil could enhance EPS production . The highest biomass and EPS production of L .calyculiforme in the batch fermentation were 6.4 0 and 8.2 8 g.L-1 respectively,but those of the fed-batch fermentation by glucose feeding were7.7 2 and11.8 2 g.L-1 respectively when the glucose concentration in the medium was lower than5 g.L-1,showing that feeding glucose can enhance greatly the accumulation of biomass and EPS of L .calyculiforme .

Published: 09 November 2010
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YE Ming
CHEN Wu-xi
PENG Wei
MA Jian
YANG Liu
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YE Ming,CHEN Wu-xi,PENG Wei,MA Jian,YANG Liu. Effects of batch and fed-batch cultures on biomass and exopolysaccharide production of Lachnum calyculiforme. Journal of Zhejiang University: Agric. & Life Sci., 2010, 36(6): 630-634.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2010.06.006     OR     http://www.zjujournals.com/agr/Y2010/V36/I6/630


分批与流加培养对萼状粒毛盘菌生物量和胞外多糖产量的影响

通过摇瓶实验对萼状粒毛盘菌(Lachnumcalyculiforme)生物量及其产胞外多糖的发酵条件进 行研究 ;并在5 L发酵罐中进行分批与流加培养,比较二者对萼状粒毛盘菌生物量及其多糖产量的影响。结果表明 :在25℃时,粒毛盘菌生物量和产胞外多糖的最佳碳、氮源及其浓度为葡萄糖30 g.L-1、 酵母膏5 g.L-1,pH值为6.0 ;10 g.L-1橄榄油能显著加速菌丝体的生长,而10 g.L-1大豆油能明显提高胞外多糖的产量 ;分批培养时粒毛盘菌的最大生物量和多糖产量分别为6.4 0和8.2 8 g.L-1,而在葡萄糖浓度低于5 g.L-1时进行流加培养可以显著提高粒毛盘菌的生物量和多糖产量,最大生物量和多糖产量分别为7.7 2和11.8 2 g.L-1 。
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