Please wait a minute...
浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2018, Vol. 44 Issue (1): 31-40    DOI: 10.3785/j.issn.1008-9209.2017.03.211
Plant protection     
Description of two new records of genus Longidorus (Nematoda: Dorylaimida) in China
BARSALOTE Eda Marie, TIAN Zhongling, CAI Ruihang, LI Xiaolin, ZHENG Jingwu*
Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Download: HTML ( )    PDF(2988KB)
Export: BibTeX | EndNote (RIS)      

Abstract  

Two new recorded species of genus Longidorus (L. pius and L. diadecturus) were recovered and collected from rhizosphere of deciduous trees in Hangzhou of Zhejiang Province and Wuhan of Hubei Province, China, respectively. Morphological identity was confirmed using the revised polytomous key of Loof & Chen, 1999. The codes for identifying the species of L. pius and L. diadecturus are A56-B4-C3-D1-E1-F3-G1-H1-I1 and A4-B23-C5-D3-F2-G2-H1-I1, respectively. L. pius was characterized by medium body size (L=4.7-6.3 mm), anterior region abruptly tapering with rounded lip continuous from body profile, odontostyle long (130-143 μm) and short rounded tail. Meanwhile, L. diadecturus could be distinguished by short body size (3.2-4.6 mm), short odontostyle (108-120 μm), posterior position of guide ring (33-38 μm), wide lip region and a relatively anterior vulva. Phylogenetically, the rDNA internal transcribed spacer 1 (ITS1) region of L. pius from China showed 99% supported clade with L. pius topotype (AM743178) from Macedonia, and the similar relationship
was obtained in L. diadecturus from China with 99% well supported clade to L. diadecturus type population (AF511416) from Canada. In addition, the 28S region of L. diadecturus from China showed 99% supported clade with L. diadecturus type specimen (AY601584), whereas L. pius noticeably clustered to L. raskii (AJ549983) and Longidorus sp. FDL-2011 (FR775757). The two species, L. pius and L. diadecturus were recorded for the first time in China, and the morphological description and phylogenetic relationships of the given species were provided in this study.

Key words： morphology      phylogeny      new record      Longidorus      China     
Received: 21 March 2017      Published: 31 May 2017
CLC:  S 432  
Fund:  Project supported by the National Natural Science Foundation of China (Nos. 31371921, 30870274)
Corresponding Authors: jwzheng@zju.edu.cn     E-mail: mail: jwzheng@zju.edu.cn
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
BARSALOTE Eda Marie
TIAN Zhongling
CAI Ruihang
LI Xiaolin
ZHENG Jingwu
Cite this article:

BARSALOTE Eda Marie, TIAN Zhongling, CAI Ruihang, LI Xiaolin, ZHENG Jingwu. Description of two new records of genus Longidorus (Nematoda: Dorylaimida) in China. Journal of Zhejiang University (Agriculture and Life Sciences), 2018, 44(1): 31-40.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2017.03.211     OR     http://www.zjujournals.com/agr/Y2018/V44/I1/31


中国长针线虫属（线虫门：矛线目）2 新记录种记述（英文）

本文记述了从浙江省和湖北省落叶树根围采集的隶属于长针线虫属的中国2 新记录种——Longidorus pius和L. diadecturus，通过修订的Loof & Chen（1999）多岐检索表确认，其鉴定代码分别为A56-B4-C3-D1-E1-F3-G1-H1-I1 和A4-B23-C5-D3-F2-G2-H1-I1。L. pius的主要形态学特征为：体长中等（4.7～6.3 mm），虫体前端明显变细，唇区圆滑，与体驱间无缢缩，齿针长（130～143 μm），尾短而圆。L.diadecturus的主要鉴别特征为：体长较短（3.2～4.6 mm），齿针长（108～120 μm），导环位置相对靠后（至唇端距离为33～38 μm），唇区宽，阴门的位置相对靠前等。系统发育研究表明：来自中国的L. pius rDNA 中ITS1 区序列与来自马其顿的模式种L. pius群体相应序列（AM743178）处于同一进化枝中，置信度达99%；L. diadecturus与来自加拿大的模式种L. diadecturus群体相应序列（AF511416）处于同一进化枝中，置信度也达99%。另外，来自中国的L. diadecturus 28S 区序列与模式种L.diadecturus的序列（AY601584）处于同一进化枝中，置信度达99%，而L. pius和L. raskii（AJ549983）、Longidorus sp.FDL-2011（FR775757）属于同一分枝。L. pius和L. diadecturus这2 个种在中国均系首次报道，本文提供了详细的形态学描述及系统发育关系的有关信息。


关键词： 系统发育,  中国,  长针线虫属,  形态学,  新记录 
[1] WANG Jiakun, HEWenfeng.

Community and functionality of fungi in the gastrointestinal tracts of herbivores revealed by omics [J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2018, 44(2): 131-139.
[2] XU Changchun, ZHENG Ge, LIN Youhua. Analysis of program for research and development on comprehensive prevention and control techniques for agricultural non-point source and heavy metal polluted croplands[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2017, 43(6): 657-662.
[3] TAN Bowen, ZHU Fanghuan, XU Jiaming, ZHANG Xinyuan, LI Duo.  Understanding motives for food choice of Chinese young people in mainland China[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2017, 43(2): 220-228.
[4] LIU Xi, CHEN Xianxing. Five plant species newly recorded in Zhejiang Province, China[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2017, 43(1): 33-36.
[5] ZHU Hongxue, TIAN Zhongling, CAI Ruihang, LI Xiaolin, ZHENG Jingwu. Heterodera koreana, a new record of cyst-forming nematodes in Zhejiang Province, China[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2017, 43(1): 81-88.
[6] SHI Xiaoping, YUAN Sen, LI Xinhua, XING Guipei . Leaf characteristics and geographical distribution of Berberis amurensis and B. anhweiensis of Berberidaceae[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 42(6): 687-693.
[7] CHEN Lichun, CHEN Zhenghai, MA Dandan, LIN Hailun, LI Xiupeng, LI Genyou. Six newly recorded plant species  in Zhejiang Province[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 42(5): 551-.
[8] TU Yanla, XIAO Fang, ZHANG Jingze, LU Qiqiang, WANG Lefu. Two “myxomycete diseases” occurred on cultivated fields of Dendrobium candidum.[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 42(2): 137-142.
[9] Zhang Xiaomei, Ding Yanfeng, Zhang Wujun, Wu Xiaoran, Li Ganghua. Spatial and temporal variation of rice yield in rice region of Southwest China.[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(6): 695-702.
[10] Zhou Zhuang, Wu Difei, Chen Xianxing, Tao Zhengming, Xiong Xianhua, Yang Yanping, Ding Bingyang. Two genera and four species newly recorded in Zhejiang Province, China.[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(2): 201-204.
[11] Hong Chuntao, Miao Lihua, Ji Mengcheng. Syntrichia laevipila Brid.： A new record species of bryophytes from Zhejiang Province[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(03): 320-322.
[12] Wu Shengjiang1, Dian Ruili2, Wei Kesu1, Li Delun1, Han Zhikang3, Lin Yechun1, Pan Wenjie1, Xie Yishu1*. Comparison of botanical characters and physiological characteristics among different flue-cured tobacco genotypes in
seedling transplanting period[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2014, 40(5): 511-518.
[13] Lin Zhangxiang， Wang Yingying， Fu Fei， Ye Chuyu， Fan Longjiang. Complete chloroplast genome of Dongxiang wild rice and its application in phylogenetic analysis[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2014, 40(4): 397-403.
[14] LIU Kaidong, YUAN Changchun*, CHEN Yan, XIAO Zhengquan, LIU Zhu. Comparison of leaf epidermis and pollen micromorphology among Dimocarpus longana and Litchi chinensis cultivars in Western Guangdong[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(6): 645-654.
[15] XIONG Xianhua1, WU Qingling2, CHEN Xianxing3, HU Renyong3, WU Yunan3, DING Bingyang3*. Two genera and five species newly recorded in Zhejiang Province, China[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(6): 695-698.