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浙江大学学报（农业与生命科学版）
  2012, Vol. 38 Issue (3): 256-262    DOI: 10.3785/j.issn.1008-9209.2012.03.004
Biological sciences & biotechnology     
Cloning and characterization of Bombyx mandarina prophenoloxidase  gene PPO1
ZHANG Yongliang1, SHENG Dongfeng1, ZHU Yong2
1. Department of Life Science, Zhoukou Normal University, Zhoukou, Henan 466001, China; 2. College of Biotechnology, Southwest University, Chongqing 400715, China
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Abstract  The complemental deoxyribonucleic acid（cDNA）of Bombyx mandarina prophenoloxidase   gene PPO1 was cloned by  reverse transcriptionpolymerase chain reaction （RTPCR）．The results showed that the cDNA with 2086 bp in length contained an open reading frame (ORF) of 2058 bp which encoded 685 amino acid residues．The deduced amino acid sequence had a high identity to the reported sequence of PPO1 from other lepidopterous insects and shared the typical structural features of PPO from other insects. The PPO1 gene was only expressed in head and blood of B. mandarina by RTPCR, and only expressed in blood by Northern blot analysis. These results provide a molecular basis for further studying the function of PPO1 gene in B. mandarina.

Published: 24 May 2012
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SHENG Dongfeng
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Cite this article:

ZHANG Yongliang, SHENG Dongfeng, ZHU Yong. Cloning and characterization of Bombyx mandarina prophenoloxidase  gene PPO1. , 2012, 38(3): 256-262.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2012.03.004     OR     http://www.zjujournals.com/agr/Y2012/V38/I3/256


野桑蚕酚氧化酶原基因PPO1的克隆及其特征(英文)

利用反转录聚合酶链式反应(RTPCR)克隆野桑蚕(Bombyx mandarina)酚氧化酶原基因PPO1，获得其cDNA序列；该序列长2086 bp，含有一个2058 bp的完整开放阅读框，编码一个由685个氨基酸残基组成的蛋白质；该基因推导的氨基酸序列与其他鳞翅目昆虫PPO1基因相应的氨基酸序列有较高的同源性，该序列具有它们的PPO基因所共有的典型特征。RTPCR检测分析表明该基因仅在野桑蚕的头部和血液中表达，而Northern杂交检测表明该基因仅在血液中有表达。这些结果为进一步研究该基因的功能提供了分子基础。
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