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浙江大学学报（农业与生命科学版）
  2012, Vol. 38 Issue (3): 250-255    DOI: 10.3785/j.issn.1008-9209.2012.03.003
Biological sciences & biotechnology     
Transformation of Lagerstroemia indica by improved Agrobacteriummediated floral-dip and pollen-tube pathway method.
CHEN Yan1， SUN Kuanying2,  ZHANG Tao2
1. College of Life Sciences, Liaocheng University, Liaocheng, Shandong 252059, China； 2. College of Agriculture, Liaocheng University, Liaocheng, Shandong 252059, China
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Abstract  Lagerstroemia indica was transformed by an improved Agrobacteriummediated floral-dip and pollen-tube pathway method, respectively, using green fluorescent protein gene (GFP) as a report gene, and its fruit number, rate of seedsetting set and seedling rate were counted. The transformed plants were screened from the 28-day seedlings irradiated by a portable ultraviolet lamp at 365 nm. The experimental results showed that the rate of seedsetting set and seedling rate by pollen-tube pathway method were significantly higher than those  by the floraldip method. The transformation rate of L. indica by the floral-dip method was higher by 0.06% than that by the pollentube pathway, which didn’t  yet reach significant levels. The rate of seedsetting set by the pollentube pathway method was higher by 11.87%-26.74% than that by the floral-dip method, indicating that the pollen-tube pathway method is more beneficial for transformation of L. indica.

Published: 24 May 2012
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SUN Kuanying
ZHANG Tao
CHEN Yan
Cite this article:

CHEN Yan， SUN Kuanying, ZHANG Tao. Transformation of Lagerstroemia indica by improved Agrobacteriummediated floral-dip and pollen-tube pathway method.. , 2012, 38(3): 250-255.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2012.03.003     OR     http://www.zjujournals.com/agr/Y2012/V38/I3/250


通过改良农杆菌介导的floral-dip法和花粉管通道法转化紫薇

以绿色荧光蛋白(GFP)基因为报告基因，分别用改良农杆菌介导的花序浸渍法（floral-dip）和花粉管通道法转化紫薇(Lagerstroemia indica)，统计果实数、结实率、成苗率，然后用紫外灯照射28 d左右的幼苗筛选转化株。结果表明：花粉管通道法转化紫薇的结实率、成苗率均明显高于floral-dip法；用手携式紫外灯（365 nm）照射进行活体鉴定，floral-dip法转化率比花粉管通道转基因法转化率高0.06%，但未达到显著水平；而花粉管通道法的结实率比floral-dip法的高11.87%~26.74%，更利于获得转化植株。说明通过花粉管通道法进行转化更适合于紫薇转基因新品种的培育。
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