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浙江大学学报（农业与生命科学版）
  2012, Vol. 38 Issue (3): 237-242    DOI: 10.3785/j.issn.1008-9209.2012.03.001
Biological sciences & biotechnology     
Transcriptional differences between two distinct manganese containing superoxide dismutase genes in Bacillus cereus.
LIU Juan1, WANG Song1, ZHANG Liqin1, WANG Yongjun1, WANG Qi2
1. School of Forestry and Biotechnology, Zhejiang A & F University, Lin′an, Zhejiang 311300, China; 2. Department of Plant Pathology, China Agricultural University, Beijing 100094, China
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Abstract  The promoter activity differences of two manganesecontaining superoxide dismutase (MnSOD) genes sodA1 and sodA2 in Bacillus cereus 905 were assayed in vivo by use of lacZ reporter. The results showed that sodA1 and sodA2 were transcripted constitutively during the bacterial growth. The promoter activity of sodA1 were 34 times higher than that of sodA2. Compared  with  wild type, both sodA1 and sodA2 were induced in a MnSODdeficient strain KOS, which suggested some unknown regularity for sodA1 and sodA2 transcriptions in response to oxidative stress. Additionally, bacterial blue colonies caused by the high βgalactosidase activity under the sodA1lacZ reporter indicated  that sodA1lacZ reporter was feasible to label B. cereus.

Published: 24 May 2012
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WANG Yongjun
WANG Qi
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Cite this article:

LIU Juan, WANG Song, ZHANG Liqin, WANG Yongjun, WANG Qi. Transcriptional differences between two distinct manganese containing superoxide dismutase genes in Bacillus cereus.. , 2012, 38(3): 237-242.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2012.03.001     OR     http://www.zjujournals.com/agr/Y2012/V38/I3/237


蜡样芽孢杆菌中锰超氧化物歧化酶基因的表达差异性

对蜡样芽孢杆菌(Bacillus cereus)905中2个不同的锰超氧化物歧化酶（MnSOD）基因（sodA1和sodA2）转录水平的差异性进行分析。结果发现：sodA1和sodA2在细菌的不同生长阶段为组成性表达，sodA1基因启动子的活性比sodA2高3~4倍；超氧化物歧化酶（SOD）的缺失能同时诱导sodA1和sodA2的转录。该研究结果解释了这2个不同的MnSOD在细胞内的酶活性差异，并推测在sodA1和sodA2基因启动子上可能存在与氧自由基代谢相关的调控元件；同时，本研究也成功实现了LacZ在芽孢杆菌上的标记。
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