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Journal of Zhejiang University: Agric. & Life Sci.  2011, Vol. 37 Issue (3): 326-331    DOI: 10.3785/j.issn.1008-9209.2011.03.014
Food sciences     
Analysis of conjugated linolenic acid from plant seeds
YUAN Gao-feng1,2,SUN Hai-yan1,LI Duo2
1 . College of Food and Medicine , Zhejiang Ocean University , Zhoushan , Zhejiang 316004 , China ; 2 . Department of Food Science and Nutrition , College of Biosystems Engineering and Food Science , Zhejiang University , Hangzhou 310029 , China
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Abstract  The objective was to determine the lipid classes and conjugated linolenic acid (CLNA) contents in T richosanthes kirilow ii , pomegranate and bitter melon seed oils from China . Three pomegranate cultivars (Sanbaiyu ,Qingpiruanzi and Tianlüzi)and six bitter melon cultivars (Changbai ,Manyouqing ,Bingchengyihao , Kaihua-1 , Kaihua-2 and Qingfeng) were chosen for analysis . The predominant lipid was triacylglycerol in the analyzed samples ranging from 92.6% to 97.0% . The major fatty acids were oleic acid (22.6% , 18 :1) , linoleic acid ( 32.6% , 18 :2n-6) and punicic acid (32.6% , c9 , t11 , c13-18 :3) in T . kirilow ii seed oil . The total CLNA was 36.9% in T . kirilow ii seed oil . Punicic acid was the most predominant fatty acid , ranging from 73.4% to 77.5% and the total CLNA ranged from 83.4% to 87.9% in the seed oils of pomegranate cultivars . The total CLNA ranged from 65.2% to 71.9% , and α-eleostearic acid ( c9 , t11 , t13‐18 :3 ) was predominant one , account from 56.0% to 63.7% in bitter melon seed oils . T . kirilow ii , pomegranate and bitter melon were rich in CLNA , however , main CLNA in T . kirilow ii and pomegranate was punicic acid , and one in bitter melon was α‐eleostearic acid . Natural resources containing CLNA , especially edible T . kirilow ii seed , could be a potential dietary source of CLA , following CLNA metabolism .

Published: 20 May 2011
Cite this article:

YUAN Gao-feng,SUN Hai-yan,LI Duo. Analysis of conjugated linolenic acid from plant seeds. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(3): 326-331.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.03.014     OR     http://www.zjujournals.com/agr/Y2011/V37/I3/326


植物种子共轭亚麻酸含量分析

以栝楼、3 个石榴品种(三白玉、青皮软籽和甜绿子)和6 个苦瓜品种(长白、满优青、槟城一号、开化白苦瓜一号、开化白苦瓜二号和青丰)的种子为材料分析其脂质组成和共轭亚麻酸含量.结果表明:所分析的植物种子脂质组成中最主要的为甘油三酯,其质量分数在92.6% 到97.0% 之间;栝楼籽油主要的脂肪酸为油酸( 22.6% ) 、亚油酸( 32.6% ) 和石榴酸( 32.6% ) ,而总的共轭亚麻酸( conjugatedlinolenic acid , CLNA)质量分数为36.9% ;石榴籽中石榴酸质量分数高达73.4% ~ 77.5% ,总的CLNA质量分数达83.4% ~ 87.9% ;苦瓜籽α‐桐酸质量分数高达56.0% ~ 63.7% ,总的CLNA 质量分数达65.2% ~ 71.9% .研究结果表明这些植物种子富CLNA ,其中栝楼籽和石榴籽中主要的CLNA 为石榴酸,而苦瓜籽中主要的CLNA 为α-桐酸.
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