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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2011, Vol. 37 Issue (3): 263-274    DOI: 10.3785/j.issn.1008-9209.2011.03.005
Biological sciences & biotechnology     
Variation analysis of S1 gene of avian infectious bronchitis virus epidemicstrains isolated from chicken flocksin Henan Province
XU Xue1,ZHAO Jun1,WANG Chuan-qing1,WANG Ze-lin1,YANG  Xia1,ZHOU Xin1,DILIBAIER Amuti2
1 .Institute of poultry Diseases , Henan Agricultural University ,Zhengzhou 450002 ,China ; 2 .AKs Vocational and Technical College , AKs , Xinjiang 843000 ,China）
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Abstract  Seven isolates of infectious bronchitis virus ( IBV) were obtained and identified from clinical infected chicken flocks in Henan Province by using the techniques of chicken embryo inoculation , reverse transcription polymerase chain reaction (RT-PCR) and tracheal organ culture ( TOC) .Spike protein gene of 7 isolates and vaccine strain H120 was amplified by RT-PCR and sequenced . The result showed that the sizes of the whole length for S1 gene of 8 IBV strains were divided into four groups : 1632, 1620,1617 and 1611 bp ,respectively .There were multisite mutations among these sequences in addition to type-specific insertion and deletion . The analysis of deduced amino acid sequences showed that sequential patterns of S protein cleavage sites of 8 IBV strains fell into three categories : HRRRR , RRFRR and RRSRR . Homology and phylogenetic analysis showed that Jin-13 isolate was respiratory-type of IBV ,and YI isolate was ArkDPI-type , and HN/SG isolate had a distant relationship to other domestic nephropathogenic-type isolates else , and HN/HL , XP/ 1/09 , XP/3 and WZL isolates had a close relationship to Shandong nephropathogenic-type isolates but had a distant relationship to HN99 isolate .

Published: 20 May 2011
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XU Xue
ZHAO Jun
WANG Chuan-qing
WANG Ze-lin
YANG Xia
ZHOU Xin
DILIBAIER Amuti
Cite this article:

XU Xue,ZHAO Jun,WANG Chuan-qing,WANG Ze-lin,YANG Xia,ZHOU Xin,DILIBAIER Amuti. Variation analysis of S1 gene of avian infectious bronchitis virus epidemicstrains isolated from chicken flocksin Henan Province. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(3): 263-274.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.03.005     OR     http://www.zjujournals.com/agr/Y2011/V37/I3/263


鸡传染性支气管炎病毒河南流行毒株S1基因的变异分析

经鸡胚接种、反转录-聚合酶链式反应(RT-PCR) 、气管环培养等方法从河南不同地区发病鸡群中先后共分离、鉴定出7株鸡传染性支气管炎病毒( IBV) .应用RT-PCR 方法对7株病毒以及IBVH120 疫苗株的S1 基因全序列进行扩增,并对其进行克隆测序及序列分析.结果显示:8株IBV 的S1基因全长分为4类,分别是1632、1620、1617和1611 bp ;序列相互之间存在多位点变异,同时存在型特异性的插入和缺失;其S 蛋白裂解位点序列模式有HRRRR 、RRFRR 和RRSRR 3种;同源性及遗传进化分析显示Jin-13为呼吸型毒株,YI 为ArkDPI 型肾型毒株,HN/SG 株与国内其他肾型分离株亲缘关系均较远;河南肾型分离株HN/HL 、XP/1/09 、XP/3 、WZL 与山东肾型分离株之间的同源性较高且其亲缘关系最近,而与河南HN99株的亲缘关系较远.说明河南地区存在不同的IBV 流行毒株,这些流行毒株在主要保护性抗原蛋白S1 基因上发生了一定的变异.
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