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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2011, Vol. 37 Issue (3): 259-262    DOI: 10.3785/j.issn.1008-9209.2011.03.004
Biological sciences & biotechnology     
Isolation and screening of  fungal strains with high ligninolytic enzyme activities
YAN Hong1,2,3,SU Jun2,YU Cai-lian3,AI Heng-yu3,ZHANG Xiao-tian3
1 . Post‐doctoral research station , Northeast Forestry University , Harbin 150040 , China ; 2 . Work Station of Science and Technique for Post-doctorate , Heilongjiang Academy of A gricultural Sciences , Harbin 150086 ,China ; 3 . College of Chemistry and Environmental Engineering , Harbin University of Science and Technology , Harbin 150040 , China
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Abstract  The 155 strains were isolated from rot-wood and soil to screen the strains with high ligninolytic enzyme activities . Nine fungi which showed color-zone in the guaiacols medium were obtained . By measuring the enzyme activity of static liquid culture filtrates , two fungal strains (termed 14-7  and 15-1 , respectively) with higher ligninase activity of those were obtained . The activities of lignin peroxidase , manganese peroxidase and laccase from fungal strain  14-7  were up to 0.087、0.060, and 0.144 U.mL-1, respectively , after 7 d fermentation ,and the ones from strain15-1 were up to 0.070,0.059, and 0.000 U.mL-1 , respectively .

Published: 20 May 2011
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YAN Hong
SU Jun
ZHANG Xiao-tian
YU Cai-lian
AI Heng-yu
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YAN Hong,SU Jun,YU Cai-lian,AI Heng-yu,ZHANG Xiao-tian. Isolation and screening of  fungal strains with high ligninolytic enzyme activities. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(3): 259-262.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.03.004     OR     http://www.zjujournals.com/agr/Y2011/V37/I3/259


高效木质素降解菌株的分离筛选

为筛选能高效降解木质素的菌株,从31份采集的朽木和土壤样品中分离纯化获得155株菌;将纯化获得的菌株接入到含愈创木酚的选择培养基上进行初筛,观察其颜色变化,根据菌落圈和变色圈直径的比值,筛选出9株可降解木质素的菌株;将这9株菌接入产酶培养基中进行液体静止培养并测定木质素酶活力,最终获得2株高产木质素酶的真菌菌株14-7 和15-1 .将这2株真菌培养7 d 后,所产木质素过氧化物酶、锰过氧化物酶和漆酶的活力分别为0.087、0.060、0.144和0.070、0.059、0.000U.mL-1.
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