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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2011, Vol. 37 Issue (3): 245-251    DOI: 10.3785/j.issn.1008-9209.2011.03.002
Biological sciences & biotechnology     
Preparation of xylo-oligosaccharides and its effect on in vitro proliferation of probiotics
LI Yan-li1,XU Shao-chun1,LIU Yong1,YANG Hui-ling2,XU Yao-xing1
1 . Institute o f Plant Protection and M icrobiology , Zhej iang A cademy o f A gricultural Sciences , H angz hou 310021 , China ; 2 . College o f Animal Science and Technology , Northwest A & F University ,Yangling , Shaanxi 712100 , China
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Abstract  Xylo-oligosaccharides ( XOS ) from corncob were prepared by microwave digestion and a commercial endoxylanase hydrolysis."One-factor-at-a-time" method was applied to optimize the reaction parameters for improving XOS concentration , and the effect of obtained XOS on in vitro proliferation of probiotics ( Bacillus subtilis and L actobacillus ) was studied . The results showed that the optimal conditions were as follows : microwave digestion pressure of 1.6 Mpa and microwave digestion time of 5 min , endoxylanase concentration of 140 U .g -1 and enzymatic reaction time for 6 h . Under optimal conditions , hydrolysis of alkali pretreated corncob powder using microwave digestion and endoxylanase produced 82.5% of XOS in the hydrolyzate , equivalent to 11.02 g.L-1  XOS . In vitro proliferations of Lactobacillus and B .subtilis were activated by 311% and183% with the addition of 0.2% and 0.4% XOS , respectively , however their growths were inhibited with increasing the amount of XOS addition .

Published: 20 May 2011
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LI Yan-li
XU Shao-chun
LIU Yong
YANG Hui-ling
XU Yao-xing
Cite this article:

LI Yan-li,XU Shao-chun,LIU Yong,YANG Hui-ling,XU Yao-xing. Preparation of xylo-oligosaccharides and its effect on in vitro proliferation of probiotics. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(3): 245-251.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.03.002     OR     http://www.zjujournals.com/agr/Y2011/V37/I3/245


低聚木糖的制备及其对益生菌体外增殖的作用

针对玉米芯微波消解‐内切木聚糖酶水解制备低聚木糖的工艺,以低聚木糖的得率为主体评价指标,通过单因素实验对影响低聚木糖得率的微波消解过程和内切木聚糖酶水解过程的因素与水平进行研究,并考察获得的低聚木糖对益生菌的体外增殖作用.结果表明:玉米芯酶法制备低聚木糖的最佳工艺条件为微波处理压力1.6 MPa ,微波处理时间5 min ,内切木聚糖酶用量140 U.g -１ ,酶解时间6 h ;在最适条件下,玉米芯酶解液中低聚木糖的得率为82.5%,质量浓度为11.02 g.L-１ ;低聚木糖对益生菌的体外增殖实验表明,低聚木糖添加量在0.2%和0.4%时,分别可以对乳酸杆菌和枯草芽孢杆菌起到最好的增殖作用,分别达到311%和183% ,添加量进一步提高反而会抑制这2种菌的生长.
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