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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2011, Vol. 37 Issue (2): 169-174    DOI: 10.3785/j.issn.1008-9209.2011.02.008
Agricultural sciences     
Screening and identification of the mutants from tworow barley cultivar ZJU3 induced by ethyl methane sulfonate
ZHANG Xiao-qin1,2,XUE Da-wei1,ZHOU Wei-hui2,WU Fei-bo2,ZHANG Guo-ping2
1 .College of Life and  Environment  Sciences , Hangzhou  Normal  University , Hangzhou  310036 , China ;2 . Department of  Agronomy , College of  Agriculture & Biotechnology , Zhejiang University ,Hangzhou 310029 , China
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Abstract   The seeds of two -row barley ( Hordeum vulgare L .) cultivar ZJU3 were treated by chemical mutagen ethyl methane sulfonate  (EMS)  to develop a mutant library . In M2 generation , biological and agronomiccharacters were examined ,and the identified mutants were validated in M3 generation . Totally 97 phenotypemutants , involved in seedling habits , maturity , leaf morphology , plant height , spike shape and spikelet fertility were obtained from 3000treated plants , with mutated frequency of 7.46% The results indicate thefeasibility of developing barley mutant population using EMS treatment and the discussion is done on forward genetics approachof genefunction study using mutant population for barley geneticimprovement .

Published: 25 March 2011
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ZHANG Xiao-qin
XUE Da-wei
ZHOU Wei-hui
WU Fei-bo
ZHANG Guo-ping
Cite this article:

ZHANG Xiao-qin,XUE Da-wei,ZHOU Wei-hui,WU Fei-bo,ZHANG Guo-ping. Screening and identification of the mutants from tworow barley cultivar ZJU3 induced by ethyl methane sulfonate. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(2): 169-174.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.02.008     OR     http://www.zjujournals.com/agr/Y2011/V37/I2/169


用甲基磺酸乙酯（EMS）诱变的大麦浙农大3号突变体的筛选和鉴定

利用化学诱变剂甲基磺酸乙酯 (ethyl methane sulfonate ,EMS) 诱变处理大麦品种浙农大3号的种子 , 然后种于大田 , 将获得的M 2代材料进行生物学性状与农艺性状鉴定 , 入选的M 3代材料继续进行验证 . 结果表明 , 实验获得了幼苗、生育期、叶、茎秆、穗及育性等生物学特性与主要农艺性状的突变体 , 变异类型丰富 , 突变群体的表型变异率约为7.46%. 本研究构建的突变群体为大麦功能基因组研究奠定了基础 , 同时为大麦遗传改良提供了重要的材料 .
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