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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2011, Vol. 37 Issue (2): 125-132    DOI: 10.3785/j.issn.1008-9209.2011.02.002
Biological sciences & biotechnology     
Polymorphism and phylogenetic analysis of ribosomal protein genes of Candidatus  Liberibacter asiaticus
LU Lian-ming,FAN Guo-cheng,YAO Jin-ai,HU Xiu-rong,CHEN Guo-qing
1 .Zhejiang Citrus Research Institute , Taizhou , Zhejiang 318020 , China ;2 . Research Institute of  Fruits , Fujian Academy of Agricultural Sciences , Fuzhou 350013 , China ;3 .Research Institute of Plant Protection , Fujian Academy of Agricultural Sciences , Fuzhou 350013 ,China
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Abstract  Ribosomal protein genes of Candidatus Liberibacter asiaticus from six different geographical regions were amplified ,and the restriction fragment length polymorphism (RFLP) of PCR products were analyzed . Then , the sequences of ribosomal protein genes were aligned with software DNAman and NCBI Blast , and the phylogenetic tree was constructed . The results showed that the ribosomal protein genes of six isolates had different RFLP fingerprints and showed some polymorphism , and the differences existed in the nucleotide sequences and amino acid sequences of ribosomal protein genes of different isolates , while the homology between each other was very high . Their homology level was from 99% to 100% with other  Ca.L.asiaticus in GenBank , 85% with  Ca.L.africanus,and 77% to 80% with Ca.L.americanus , respectively . Phylogenetic analysis suggested that all Ca.L.asiaticus were clustered into one group , and then clustered in a larger group as Candidatus Liberibacter with  Ca.L.africanus and  Ca.L.americanus.

Published: 25 March 2011
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LU Lian-ming
FAN Guo-cheng
YAO Jin-ai
HU Xiu-rong
CHEN Guo-qing
Cite this article:

LU Lian-ming,FAN Guo-cheng,YAO Jin-ai,HU Xiu-rong,CHEN Guo-qing. Polymorphism and phylogenetic analysis of ribosomal protein genes of Candidatus  Liberibacter asiaticus. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(2): 125-132.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.02.002     OR     http://www.zjujournals.com/agr/Y2011/V37/I2/125


柑橘黄龙病菌核糖体蛋白基因的多态性及系统发育分析

通过 PCR 扩增6 个不同地区柑橘黄龙病 (citrus huanglongbing ,HLB) 病原菌的核糖体蛋白基因 , 采用4种不同的限制性内切酶对 PCR 产物进行限制性片段长度多态性 (RFLP) 分析 ; 并对核糖体蛋白基因克隆和测序后 , 使用 DNAman 和 NCBI Blast 等软件对基因序列进行比对分析 , 并通过软件 Mega4.1构建其系统发育树 . 结果表明 : 6个分离物核糖体蛋白基因的 RFLP 指纹图谱有所差异 , 表现出一定的多态性 ; 不同分离物核糖体蛋白基因的核酸序列和氨基酸序列均存在差异 , 但相互之间同源性很高 ,其中核酸序列与数据库中亚洲种 ( Candidatus Liberibacter asiaticus) 的同源性高达99% ~100% , 与非洲种 ( Ca.L.africanus) 的同源性为85% , 与美洲种 ( Ca.L.americanus) 的同源性为77% ~80% ; 系统发育分析显示所有的 Ca.L.asiaticus 亲缘关系极近 , 归为一个类群 , 而后与 Ca.L.africanus 和 Ca.L.americanus 组成韧皮部杆菌属 ( Candidatus Liberibacter) 一个大的类群 .
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