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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2011, Vol. 37 Issue (2): 119-124    DOI: 10.3785/j.issn.1008-9209.2011.02.001
Biological sciences & biotechnology     
SCAR marker linked to resistance gene of powdery mildew in bottle gourd [ Lagenaria siceraria (Molina) Standl.]  breeding line J083
WANG Ling-ping,WU Xiao-hua,WANG Bao-gen，XU Pei,LI Guo-jing
Institute of Vegetables ,Zhejiang Academy of Agricultural Sciences , Hangzhou 310021 ,China
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Abstract  The genetic analysis of bottle gourd [ Lagenaria siceraria (Molina) Standl .] resistance to powdery mildew was evaluated with a highly resistant breeding line J083 and a highly susceptible breeding line J73 and their F1 ,F2 populations .The results showed that the resistance to powdery mildew in J083 was controlled by a single recessive gene . From 100 amplified fragment length polymorphism (AFLP) primer pair combinations ,a pair of stable polymorphic AFLP markers (E -ATG/M-CTC) , 105 bp in size was obtained . The AFLP fragment was then converted to a sequence-characterized amplified region (SCAR) marker ,named as GPDS ATG/CTC75 .This SCAR marker could be used effectively for molecular marker ‐assisted selection (MAS) inbreeding programs to developbottlegourdcultivars resistance to powdery mildew .

Published: 25 March 2011
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XU Pei
LI Guojing
WANG Lingping
WU Xiaohua
WANG Baogen
Cite this article:

WANG Lingping,WU Xiaohua,WANG Baogen，XU Pei,LI Guojing. SCAR marker linked to resistance gene of powdery mildew in bottle gourd [ Lagenaria siceraria (Molina) Standl.]  breeding line J083. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(2): 119-124.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.02.001     OR     http://www.zjujournals.com/agr/Y2011/V37/I2/119


与瓠瓜品系‘J083’白粉病抗性基因连锁的SCAR分子标记

以瓠瓜抗白粉病品系 J083和感病品系J73及它们的 F1 代和F2 代分离群体为试验材料 , 经接种鉴定和抗性遗传规律分析表明 : 瓠瓜品系J083 对白粉病的抗性受单隐性基因控制 ; 从100 对扩增片段长度多态性 (AFLP) 引物组合中获得稳定的多态性引物组合1对 , 即 E ‐ ATG/M ‐ CTC ; 经回收、测序 , 特异片段全长为105bp , 并成功将其转化为序列特征性扩增区域 (SCAR) 标记 ; 经连锁分析 , 该SCAR 标记与白粉病抗性基因的连锁距离为9.6cM , 将其命名为 GPDS ATG/CTC75 . 此标记可用于瓠瓜抗白粉病品种的辅助选育 .
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