| Resource & environmental sciences |
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| Fourier transform infrared (FTIR) spectroscopic characteristics of ornamental plant leaves in response to formaldehyde stress |
| CHENG Qin,HUANG Shu-shi,MEI Yan,XU Di,CHEN Li-mei |
| CHENG Qin,Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China;Lab of Biophysics,Guangxi Academy of Sciences,Nanning530007,China HUANG Shu-shi,Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China; MEI Yan,Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China; XU Di,Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China; CHEN Li-mei,Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China; |
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Abstract To provide more evidence for selection of plant species with strong formaldehyde (HCHO)-absorbing and-purifying capacity,the HCHO-absorbing and-metabolizing capacity and the physiological characteristics of two ornamental plants,Philodendron panduriforme and Cymbidium grandiflorium,under2 and4 mmol.L-1 HCHO stress conditions were studied by the method of FTIR combined with conventional physiological approach . The results showed that the HCHO-absorbing and-metabolizing capacity of C . grandiflorium leaves was about 2 fold greater than that of P . panduriforme . P . panduriforme and C. grandiflorium had different physiological pattern in response to HCHO stress . P . panduriforme had a dramatic response to HCHO stress in a very short period (1 d) after HCHO treatment and augmented its HCHO-metabolism and-resistance ability by increasing the synthesis of substances in leaf cells .Contrary,the response of C . grandiflorium to HCHO stress was not obvious and the HCHO-metabolism and-resistance ability was increased by consuming the storage materials in leaf cells . There was a better relativity between the time for completely absorbing HCHO by P panduriforme and C. grandiflorium leaves after2 and4 mmol.L-1 HCHO treatment and the time for the recovery of each band height to normal level in FTIR spectra . Under HCHO stress conditions,the change trends of total soluble protein and soluble sugar contents in the two plant leaves basically corresponded with those shown by FTIR data,suggesting that FTIR data could reflect the physiological characteristics of the two plant leaves in response to HCHO stress . Therefore,FTIR analysis can be applied to investigate the changes in physiological characteristics of plants under HCHO stress conditions and the ability of plants to absorb and metabolize HCHO .
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Published: 09 November 2010
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用红外光谱研究植物响应甲醛胁迫的生理特性
采用傅里叶变换红外(FTIR)光谱技术结合常规生理学方法分析2种观赏植物琴叶喜林芋 ( Philodendron panduriforme)和大花蕙兰(Cymbidium grandiflorium)叶片吸收和代谢甲醛的能力及 它们响应甲醛胁迫的生理特性 。结果表明 :2种植物对甲醛胁迫产生的生理响应模式不同,琴叶喜林芋 在甲醛胁迫的短时间内作出激烈响应,并通过增加物质合成来提高其代谢和抵抗甲醛的能力,而大花蕙 兰对甲醛胁迫的响应不明显,主要通过消耗自身原来的物质来代谢和抵抗甲醛 ;2种植物完全吸收处理 液中的甲醛所需时间与它们在 FTIR光谱中各组分恢复到正常水平所需时间有较好的相关性 ;在甲醛 胁迫下这2种植物叶片中可溶性总蛋白和可溶性总糖含量的变化趋势和 FTIR数据大致相符,说明 FTIR数据能反映这2种植物叶片受甲醛胁迫后的生理响应特征 。以上研究表明,FTIR分析可以应用 于植物响应甲醛胁迫的生理特性和吸收代谢能力的研究 。
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