| Biological sciences & biotechnology |
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| Screening of plant endophytic actinomycetes producing chitinase and its antagonistic activity against Sclerotinia sclerotiorum |
| GAO Xiao-ning,Gulpiye,WANG Mei-ying,HUANG Li-li,TU Xuan,KANG Zhen-sheng |
| GAO Xiao-ning,Shaanxi Key Lab of Molecular Biology for Agriculture,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi712100,China Gulpiye,Shaanxi Key Lab of Molecular Biology for Agriculture,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi712100,China WANG Mei-ying,Shaanxi Key Lab of Molecular Biology for Agriculture,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi712100,China HUANG Li-li,Shaanxi Key Lab of Molecular Biology for Agriculture,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi712100,China TU Xuan,Shaanxi Key Lab of Molecular Biology for Agriculture,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi712100,China KANG Zhen-sheng,Shaanxi Key Lab of Molecular Biology for Agriculture,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi712100,China |
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Abstract One hundred and eighty-six endophytic actinomycetes strains isolated from nine kinds of plants were screened for chitinase production by showing the transparent zone on the selective media containing chitin,and a high-yield strain GKSHJA of chitinase isolated from the stem of Polygonatumsibirium was obtained,whose antifungal activity on some common plant pathogens was evaluated by using liquid shake culture . The results showed that strain GKSHJA had high inhibition efficiency not only on Sclerotinia sclerotiorum with 85.4 % inhibition rate to mycelial growth,but also on other 10 kinds of phytopathogens . The strain GKSHJA was identified as Streptomyces longisporoflavus based on morphological,physiological and biochemical,and cytochemical characteristics . The medium with initial pH 6,containing 1% floury chitin as carbon source and 0.3 0% beef extract as nitrogen source,incubated at32℃for 6 d with 150 r.m in-1,was recommended for chitinase production of the strain GKSHJA .
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Published: 09 November 2010
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产几丁质酶内生放线菌的筛选及其对核盘菌的抑制作用
采用透明圈法对分离自9种植物的186株高产几丁质酶内生放线菌进行筛选,获得1株分离 自黄精块茎的菌株 GKSHJA,用液培法测定其对病原菌的抑制效果。结果表明:该菌株对油菜菌核病菌 的抑制率为85.4 %,该菌的粗酶液对其他10种常见植物病原菌菌丝生长具有不同程度的抑制效果;依据形态学特征、生理生化特性和细胞化学分析,该菌株被鉴定为黄色长孢链霉菌 ( Streptomyces longisporoflavus);对 GKSHJA菌株产酶条件的研究结果表明,当碳源为1%的粉状几丁质,氮源为 0.3 0%的牛肉膏,初始 pH 6,32℃培养6 d时,培养液中几丁质酶活性较高 。
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