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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2010, Vol. 36 Issue (5): 521-527    DOI: 10.3785/j.issn.1008-9209.2010.05.007
Biological sciences & biotechnology     
Isolation and identification of a decabromodiphenyl ether degrading bacterium BD-24 and its degradation characteristics
CHEN Xue- jiao1,2,JIN Dan-feng1,DING Hai- tao1,ZHAO Yu-hua1
1.Institute of Microbiology,College of Life Sciences,Zhejiang University,Hangzhou 310058,China; 2.Taizhou Academy of Agricultural Sciences,Linhai,Zhejiang317000,China
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Abstract  A bacterial strain BD-24,which was able to use decabromodiphenyl ether (BDE-209) as the sole carbon and energy source,was isolated from activated sludge in an oil refinery at Hangzhou,China by selective enrichment method .Based on morphological,physiological and biochemical characteristics,phylogenetic analysis of 16S rDNA gene sequence similarity,and (G + C) mol%,strain BD-24 was identified as Bacillussp . High performance liquid chromatograph analysis showed that the residue rate of BDE-209 (20 mg.L-1 ) in mineral medium decreased to75.1 % and65.6 % after the incubation of strain BD-24 for15 and25 days,respectively . Addition of second carbon sources generally enhanced the BDE-209 degradation by strain BD-24,especially in which maltose could result in 20 mg.L-1 of BDE-209 decreasing quickly to83.5 % within5 days . No active effect of different kinds of nitrogen sources on the BDE-209 biodegradation was observed .

Published: 13 September 2010
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CHEN Xue- jiao1
2
JIN Dan-feng1
DING Hai- tao1
ZHAO Yu-hua1
Cite this article:

CHEN Xue- jiao1,2,JIN Dan-feng1,DING Hai- tao1,ZHAO Yu-hua1. Isolation and identification of a decabromodiphenyl ether degrading bacterium BD-24 and its degradation characteristics. Journal of Zhejiang University: Agric. & Life Sci., 2010, 36(5): 521-527.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2010.05.007     OR     http://www.zjujournals.com/agr/Y2010/V36/I5/521


十溴二苯醚降解菌的分离鉴定及其降解特性的初步研究

通过选择性富集法从杭州炼油厂附近长期受石油污染的土壤中分离到1株以十溴二苯醚为唯一碳源、能源生长的细菌 BD-24,经形态、生理生化、16S rDNA核苷酸序列分析,以及(G+ C)mol% 测定,菌株 BD-24被鉴定为芽孢杆菌属(Bacillus sp.) ;并用高效液相色谱法测定十溴二苯醚浓度 。结 果表明 :菌株 BD-24在含20 mg.L-1十溴二苯醚的无机盐培养基上培养15 d,十溴二苯醚残余率下降 至75.1 %,到第25天,残余率则为65.6 % ;不同种类碳源的添加均能不同程度地促进十溴二苯醚的 降解,其中麦芽糖作用最为显著,培养5 d后,残余率仅剩83.5 % ;而不同氮源的添加对降解无显著 作用 。
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