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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2004, Vol. 30 Issue (4): 430-430    
论文     
Study on the character of chitinase produced by Trichoderna spp. with measuring reducing sugar
LIU Kai-qi　 XIANG Mei-mei　 LIU Ren　 ZENG Yong-san　 LI Hua　 JIANG Xin-yin　 ZHANG Yue-li　
LIU Kai-qi（Dept.of Resource and Environmental Science, Zhongkai Agrotechnical College, Guangzhou 510225, China）　　　　　　
XIANG Mei-mei（Dept.of Resource and Environmental Science, Zhongkai Agrotechnical College, Guangzhou 510225, China）　　　　　　
LIU Ren（Dept.of Resource and Environmental Science, Zhongkai Agrotechnical College, Guangzhou 510225, China）　　　　　　
ZENG Yong-san（Dept.of Resource and Environmental Science, Zhongkai Agrotechnical College, Guangzhou 510225, China）　　　　　　
LI Hua（College of Plant Protection, Shandong Agric. Univ., Tai' an 271018, China）　　　　　　
JIANG Xin-yin（College of Plant Protection, Shandong Agric. Univ., Tai' an 271018, China）　　　　　　
ZHANG Yue-li（College of Plant Protection, Shandong Agric. Univ., Tai' an 271018, China）　
 全文: PDF(34 KB)  
Abstract: A trusty and intuitionistic method for screening chitinase produced by Trichoderma spp. was developed. 38 isolates of Trichoderma spp. were cultured in liquid medium with chitin or colloidal chitin as the sole carbon source for 4 days. The supernatant of the fermented broth was mixed with colloidal chitin and heated in water-bath at 37℃ for 30 min, then 3,5-dinitrosalicylic acid reagent (DNS) was added to the mixture, and let them react for 10 min in water-bath. According to the different colour of the mixture, the isolates of Trichoderma spp. which can produce chitinase could be screened.……
出版日期: 2004-07-12
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引用本文:

LIU Kai-qi　 XIANG Mei-mei　 LIU Ren　 ZENG Yong-san　 LI Hua　 JIANG Xin-yin　 ZHANG. Study on the character of chitinase produced by Trichoderna spp. with measuring reducing sugar [J]. 浙江大学学报(农业与生命科学版), 2004, 30(4): 430-430.

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https://www.zjujournals.com/agr/CN/Y2004/V30/I4/430

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