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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2004, Vol. 30 Issue (4): 426-426    
论文     
Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum
DAI Fu-ming　 XU Tong　
DAI Fu-ming（Institute of Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang ,China;Plant Protection Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China）　　　　　　
XU Tong（Institute of Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang ,China）　
 全文: PDF(41 KB)  
Abstract: Sclerotinia sclerotiorum is an important pathogen to many crops and is especially damaging to rape in China. As a model plant Arabidopsis thaliana (ColO) was transformed by spraying Agrobacterium tumefacience with Trichoderma endochitinase gene ThEn-42 at initial bud stage. Eleven seedlings (corresponding to about 0.22 percent transformation) exhibited resistance to hygromycin. The DNA fragment unique to endochitinase ( ThEn-42 ) was amplified by Arabidopsis leaf-PCR or genomic DNA PCR. Unfertile, dwarf and normal phenotypes appeared in the T1 generation. In addition, an enhanced resistance to S. sclerotiorum was observed. The mortality percentage (7.7% to 33.3%) in transgenic plants was significantly lower than in non-transgenic plants (86. 7%) 10 days after inoculation with the pathogen.……
出版日期: 2004-07-12
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引用本文:

DAI Fu-ming　 XU Tong　. Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum[J]. 浙江大学学报(农业与生命科学版), 2004, 30(4): 426-426.

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https://www.zjujournals.com/agr/CN/Y2004/V30/I4/426

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