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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2004, Vol. 30 Issue (4): 418-418    
论文     
Heterologous expression of the glucose oxidase gene in Trichoderma atroviride leads enhanced ability to attack phytopathogenic fungi and induction of plant systemic disease resistance
Robert L Mach　 Brunner Kurt　 Matteo Lorito　 Susanne Zeilinger　 Rosalia Ciliento　 Sheridan Woo　
Robert L Mach（Gene Technology Group, Institute of Chemical Engineering, TU- Vienna, Getreidemarkt 9/166/5, A-1060 Wien, Austria）　　　　　　
Brunner Kurt（Gene Technology Group, Institute of Chemical Engineering, TU- Vienna, Getreidemarkt 9/166/5, A-1060 Wien, Austria）　　　　　　
Matteo Lorito（Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Sezione di. Patologia Vegetale, Universita degli Studi di Napoli "Federico Ⅱ", Via Universita,100, 80050. Portici (NA ), Italy）　　　　　　
Susanne Zeilinger（Gene Technology Group, Institute of Chemical Engineering, TU- Vienna, Getreidemarkt 9/166/5, A-1060 Wien, Austria）　　　　　　
Rosalia Ciliento（Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Sezione di. Patologia Vegetale, Universita degli Studi di Napoli "Federico Ⅱ", Via Universita,100, 80050. Portici (NA ), Italy）　　　　　　
Sheridan Woo（Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Sezione di. Patologia Vegetale, Universita degli Studi di Napoli "Federico Ⅱ", Via Universita,100, 80050. Portici (NA ), Italy）
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Abstract: 　A transgenic strain of Trichoderma atroviride that expresses the Aspergillus niger glucose oxidase gene goxA under a homologous pathogen-inducible promoter (nag1) has been constructed, with the aim of increasing the ability of this biocontrol agent (BCA) to attack phytopathogenic fungi and enhance plant systemic disease resistance. The sporulation and growth rate of the transgenic progenies were similar to the wild-type strain Pl. goxA expression occurred immediately after contact with the plant pathogen,and the glucose oxidase formed was secreted extracellularly. The transformed strain SJ3 4, containing 12-14 copies of the transgene, produced significantly less N-acetyl-glucosaminidase and endochitinase then wild type. However, the ability of its culture filtrate to inhibit the germination of Botrytis cinerea spores was increased by about 3-fold. In comparison to P1, the transgenic strain more quickly overgrew and lysed in vitro the pathogens Rhizoctonia solani and Pythium ultimum.……
出版日期: 2004-07-12
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引用本文:

Robert L Mach　 Brunner Kurt　 Matteo Lorito　 Susanne Zeilinger　 Rosalia Ciliento　. Heterologous expression of the glucose oxidase gene in Trichoderma atroviride leads enhanced ability to attack phytopathogenic fungi and induction of plant systemic disease resistance[J]. 浙江大学学报(农业与生命科学版), 2004, 30(4): 418-418.

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https://www.zjujournals.com/agr/CN/Y2004/V30/I4/418

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