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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2019, Vol. 45 Issue (3): 278-287    DOI: 10.3785/j.issn.1008-9209.2018.10.261
生物科学与技术     
简单重复序列标记和序列相关扩增多态性标记在草莓遗传多样性分析中的比较
忻雅1(),方献平1,2,王淑珍1,童建新1,来文国1,汪建荣1,余红1()
1. 杭州市农业科学研究院，杭州 310024
2. 上海市农业科学院林木果树研究所，上海 201403
Comparison of simple sequence repeat (SSR) and sequence related amplified polymorphism (SRAP) markers for genetic diversity analysis in strawberry
Ya XIN1(),Xianping FANG1,2,Shuzhen WANG1,Jianxin TONG1,Wenguo LAI1,Jianrong WANG1,Hong YU1()
1. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China
2. Institute of Forestry and Pomology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
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摘要：

利用简单重复序列（simple sequence repeat, SSR）和序列相关扩增多态性(sequence related amplified polymorphism, SRAP)标记分析了来自国内外43个草莓品种的遗传多样性，并比较了这2种分子标记的效果差异。结果表明：30对SSR引物平均多态性位点数为6.43个，每个位点的平均多态性信息含量（polymorphism information content, PIC）值为0.628 4；20个SRAP引物组合平均多态性位点数为14.30个，PIC值高达0.911 4。基于SSR标记的聚类结果与基于SRAP标记的聚类结果的相关系数为0.817，呈显著水平。而SSR标记、SRAP标记分别与SSR+SRAP联合标记的相关系数为0.938和0.966，都达到极显著水平。SSR和SRAP标记都能较好地分析草莓遗传多样性，其中SRAP标记的效果略优于SSR标记，而SSR+SRAP联合标记分析则能更好地评估种质的遗传多样性和亲缘关系。
关键词： 草莓;  简单重复序列;  序列相关扩增多态性;  遗传多样性    
Abstract:

Simple sequence repeat (SSR) and sequence related amplified polymorphism (SRAP) markers were applied to analyze genetic diversity among 43 strawberry (Fragaria×ananassa Duch.) cultivars and effectivenesses of these two kinds of molecular markers were also compared. The results showed that there were 6.43 polymorphic sites per primer pair of 30 SSR primers, and the average polymorphism information content (PIC) of each site was 0.628 4. While there were 14.30 polymorphic sites per primer pair of 20 SRAP primers，and the average PIC of each site was 0.911 4. The correlation coefficient between clustering results based on SSR markers and SRAP markers was 0.817, which was significant. The correlation coefficients between SSR markers, SRAP markers and SSR+SRAP joint markers were 0.938 and 0.966, respectively, which reached extremely significant levels. Both SSR and SRAP markers can be used to analyze the genetic diversity of strawberry, but the effect of SRAP marker is better than that of SSR marker. The analysis of SSR+SRAP joint markers can better evaluate the genetic diversity and genetic relationship of strawberry germplasm.
Key words: strawberry    simple sequence repeat (SSR)    sequence related amplified polymorphism (SRAP)    genetic diversity
收稿日期: 2018-10-26 出版日期: 2019-06-25
CLC:  S 668.4  
基金资助: 杭州市农业和社会发展科研主动设计项目(20162012A02);浙江省农业新品种选育重大科技专项(2016C02052-8);浙江省基础公益研究计划(LGN18C130002)
通讯作者: 余红     E-mail: xinya0@yeah.net;yhtlzj12345@126.com
作者简介: 忻雅（https://orcid.org/0000-0002-8459-4963），E-mail：xinya0@yeah.net
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引用本文:

忻雅,方献平,王淑珍,童建新,来文国,汪建荣,余红. 简单重复序列标记和序列相关扩增多态性标记在草莓遗传多样性分析中的比较[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(3): 278-287.

Ya XIN,Xianping FANG,Shuzhen WANG,Jianxin TONG,Wenguo LAI,Jianrong WANG,Hong YU. Comparison of simple sequence repeat (SSR) and sequence related amplified polymorphism (SRAP) markers for genetic diversity analysis in strawberry. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(3): 278-287.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2018.10.261    或    http://www.zjujournals.com/agr/CN/Y2019/V45/I3/278

编号

No.

 品种Cultivar

类型

Type

编号

No.

品种

Cultivar

类型

Type
1 章姬 日本品种 23 阿尔比 美国品种（日中性）
2 幸香 日本品种 24 波拉特 美国品种（日中性）
3 红玉 国内选育品种 25 卡姆罗莎 美国品种
4 宁玉 国内选育品种 26 达赛 法国品种
5 晶瑶 国内选育品种 27 法国1号 法国品种
6 红颊 日本品种 28 法国2号 法国品种
7 丰香 日本品种 29 法国3号 法国品种
8 甜查理 美国品种 30 京藏香 国内选育品种
9 越心 国内选育品种 31 京桃香 国内选育品种
10 越丽 国内选育品种 32 妙香 国内选育品种
11 香野 日本品种 33 黔莓 国内选育品种
12 阿玛奥 日本品种 34 艳丽 国内选育品种
13 弥生姬 日本品种 35 中莓1号 国内选育品种
14 初恋情人 日本品种（白草莓） 36 中莓3号 国内选育品种
15 桃熏 日本品种（十倍体） 37 白雪公主 国内选育品种（白草莓）
16 野生白袍 野生型 38 京郊小白 国内选育品种（白草莓）
17 野生白草莓 野生型 39 华艳 国内选育品种
18 芦根 野生型 40 香蕉草莓 国内选育品种
19 点雪 日本品种 41 粉红公主 国内选育品种
20 甘露 日本品种 42 俏佳人 国内选育品种
21 圣安德瑞斯 美国品种（日中性） 43 圣诞红 韩国品种
22 蒙特瑞 美国品种（日中性）
表1  供试草莓材料

标记

Markers

条带数

Number of bands

多态性条带数

Number of polymorphic bands

多态性比率

Polymorphic rate/%

基因型数

Number of genotypes

多态性信息含量

PIC
平均 Average 7.77 6.43 82.83 10.2 0.628 4
SB1 9 8 88.89 12 0.728 0
SB3 6 4 66.67 6 0.698 8
SB4 10 10 100.00 14 0.834 0
SB5 9 7 77.78 10 0.766 9
SB6 9 8 88.89 15 0.799 4
SB9 5 3 60.00 3 0.374 3
SB10 8 4 50.00 9 0.815 6
SB12 6 4 66.67 5 0.253 1
SB13 8 5 62.50 9 0.806 9
SB15 6 6 100.00 7 0.366 7
SB16 8 8 100.00 26 0.936 7
SB18 11 10 90.91 18 0.831 8
SB19 10 5 50.00 9 0.761 5
SB21 8 5 62.50 4 0.252 0
SB22 3 2 66.67 3 0.310 4
SB23 17 16 94.12 29 0.955 1
SB24 6 6 100.00 15 0.825 3
SB25 5 4 80.00 4 0.554 9
SB26 8 5 62.50 8 0.601 4
SB27 7 6 85.71 14 0.850 2
SB28 8 8 100.00 19 0.909 7
SB29 4 4 100.00 5 0.325 6
SB30 13 13 100.00 17 0.890 2
SB31 4 4 100.00 2 0.045 4
SB33 5 4 80.00 6 0.498 6
SB34 11 9 81.82 18 0.889 1
SB35 2 1 50.00 2 0.129 8
SB36 7 6 85.71 2 0.467 3
SB37 7 6 85.71 5 0.660 9
SB38 13 12 92.31 9 0.712 8
表2  不同SSR标记的多态性

标记

Markers

条带数

Number of bands

多态性条带数

Number of polymorphic bands

多态性比率

Polymorphic rate/%

基因型数

Number of genotypes

多态性信息量

PIC
平均 Average 16.20 14.30 88.27 27.8 0.911 4
F1R1 22 19 86.36 37 0.969 2
F1R3 13 12 92.31 37 0.969 2
F1R4 13 11 84.62 31 0.948 6
F2R1 16 15 93.75 37 0.969 2
F2R4 21 19 90.48 34 0.960 5
F3R2 20 20 100.00 39 0.969 2
F3R3 15 12 80.00 19 0.910 8
F3R4 18 18 100.00 29 0.944 3
F4R1 16 15 93.75 37 0.970 3
F4R2 11 5 45.45 8 0.708 6
F4R3 16 16 100.00 36 0.967 0
F5R1 19 16 84.21 40 0.972 4
F5R2 15 14 93.33 21 0.917 3
F5R4 12 11 91.67 11 0.808 0
F6R2 21 19 90.48 43 0.976 7
F7R2 15 11 73.33 22 0.934 6
F7R3 15 12 80.00 12 0.755 0
F8R2 8 7 87.50 20 0.844 8
F8R3 19 18 94.74 25 0.871 8
F8R4 19 16 84.21 18 0.859 9
表3  不同SRAP标记的多态性
图1   43个草莓品种的SSR标记聚类图
图2   43个草莓品种的SRAP标记聚类图
图3   43个草莓品种的SSR和SRAP标记聚类图
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