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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2016, Vol. 42 Issue (6): 665-670    DOI: 10.3785/j.issn.1008-9209.2016.01.271
生物科学与技术     
饮用水管网生物膜中弧菌数量影响因素研究
史亚1， 施晓峰1， 张永静1， 柳景青2， 程东庆1*
1.浙江中医药大学医学技术学院，杭州 310053；2.浙江大学建筑工程学院，杭州 310058
Effects of drinking water distribution system on Vibrio amounts in biofilm
SHI Ya1, SHI Xiaofeng1, ZHANG Yongjing1, LIU Jingqing2, CHENG Dongqing1*
(1. College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou 310053, China; 2. College of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310058, China)
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摘要： 研究饮用水管网中管材、管龄、管径等因素对生物膜弧菌数量的影响。采集中国东部某市管网生物膜样本12份，用硫代硫酸盐-柠檬酸盐-胆盐-蔗糖琼脂培养基分离培养弧菌，并用弧菌科细菌生化鉴定系统鉴定并计数。结果表明，弧菌检出率为50%（6/12）。在管龄相近的不同管材管壁生物膜中，弧菌数量为球墨铸铁管（212±39.40 CFU/cm2）＞镀锌钢管（4.85±1.03 CFU/cm2）＞不锈钢复合管（0.66±0.21 CFU/cm2），并且球墨铸铁管中的弧菌数量与其他管材间差异有统计学意义（P＜0.05），而在塑料管中未检出弧菌；而不同管龄的球墨铸铁管以及镀锌钢管生物膜中弧菌数量变化不明显；在不同管径（公称直径为200、150和300 mm）的球墨铸铁管生物膜中,弧菌数量分别为2.65×103±204、212±39.40和44.20±6.88 CFU/cm2。综上表明，管材对生物膜弧菌数量有较大的影响，其中，以球墨铸铁管中的弧菌数量最多；因此，饮用水管段的铺设应考虑铸铁管的潜在弧菌风险。
Abstract: With the development of modern industries, drinking water in residential area and public building were supplied by water distribution systems. The drinking water distribution system is the final step from water plants to the users, and therefore is essential to assure drinking water safety for customers. Previous studies confirmed that many bacteria in the drinking water distribution systems existed as biofilm, which could induce corrosion and scaling of the pipe walls, reduce the water quality and water-carrying capacity of the pipelines. Moreover, as the pathogens growing in the biofilms transferred in the pipelines, the risks caused by these microorganisms would increase. Vibrio species is an estuarine bacterium widely distributed in the natural aquatic environment around the world. Among the discovered 76 kinds of Vibrio species, at least 12 kinds have pathogenic effect on human, and often result in severe diarrhea and dehydration. We found the Vibrio species in our previous survey of pathogenic bacteria in biofilm, indicating that the residents were at potential risk from Vibrio species. Therefore, the effect of pipe materials, pipe ages, and pipe diameters on amount of Vibrio species in biofilm of drinking water distribution system was necessary to investigate. 
In this study, 12 biofilm samples were collected from the drinking water distribution system in east China. Vibrio was isolated using thiosulfate-citrate-bile salts-sucrose (TCBS) agar medium and was identified according to their biochemical reaction characteristics using biochemical identification kit for Vibrionaceae GYZ9V. The amounts of total bacteria were determined by a spreadplating method according to the Ministry of Health Standard Examination Methods for Drinking Water (2006). Samples and their dilutions were spread on Petri dishes with nutrient agar medium. Analysis of variance (ANOVA) by a least significant difference test was used to compare Vibrio counts from different pipe materials, ages and diameters.
The results showed that Vibrio species could be detected in nodular cast iron, galvanized steel and stainless steel clad pipes, but not in plastic pipe. The Vibrio species amount on the biofilm attached to the nodular cast iron pipe (212±39.40 CFU/cm2) was significantly higher than those attached to the galvanized steel pipe (4.85±1.03 CFU/cm2) and stainless steel clad pipe (0.66±0.21 CFU/cm2). However, the rank of total bacteria amount was galvanized steel pipe > nodular cast iron pipe ≈ plastic pipe > stainless steel clad pipe. Pipe age had little influence on Vibrio amounts from galvanized steel and nodular cast iron pipes. Nodular cast iron pipe with a nominal diameter of 200 mm had the highest amount of Vibrio species (2.65×103±204 CFU/cm2), followed by 150 mm (212±39.40 CFU/cm2) and 300 mm (44.20±6.88 CFU/cm2), and the tendency of total bacteria amount was similar with Vibrio species. 
In conclusion, contamination from Vibrio species with varying levels is observed in nodular cast iron, galvanized steel and stainless steel clad pipes, but not in plastic pipe. The different pipe materials, especially nodular cart iron pipe, have strong influence on Vibrio species amount in biofilm from drinking water distribution system; therefore, the potential risks of Vibrio species from nodular cast iron pipe should be taken into consideration while laying the pipelines.
出版日期: 2016-11-19
CLC:  R 123  
基金资助: 水体污染控制与治理国家科技重大专项(2012ZX07403-003)
通讯作者: 程东庆(http://orcid.org/0000-0002-2025-6420),E-mail:B2006069@163.com   
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史亚
施晓峰
程东庆
柳景青
张永静

引用本文:

史亚， 施晓峰， 张永静， 柳景青， 程东庆. 饮用水管网生物膜中弧菌数量影响因素研究[J]. 浙江大学学报(农业与生命科学版), 2016, 42(6): 665-670.

SHI Ya, SHI Xiaofeng, ZHANG Yongjing, LIU Jingqing, CHENG Dongqing. Effects of drinking water distribution system on Vibrio amounts in biofilm. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 42(6): 665-670.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2016.01.271    或    http://www.zjujournals.com/agr/CN/Y2016/V42/I6/665

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