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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2016, Vol. 42 Issue (3): 385-390    DOI: 10.3785/j.issn.1008-9209.2015.06.113
动物科学     
2个云南原始鸡种遗传多样性及其与红色原鸡的亲缘关系
陆俊贤, 贾晓旭, 唐修君, 樊艳凤, 唐梦君, 高玉时, 苏一军
江苏省家禽科学研究所,江苏 扬州225125
Genetic diversity of two local Yunnan chicken breeds and their relationships with red jungle fowl
Junxian, JIA Xiaoxu, TANG Xiujun, FAN Yanfeng, TANG Mengjun, GAO Yushi, SU Yijun
Jiangsu of Poultry Science, Yangzhou 225125, Jiangsu, China
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摘要： 对60只个体的线粒体基因组(mitochondrial genome,mtDNA)D-loop区全序列进行聚合酶链反应(polymerase chain reaction,PCR)扩增和测序,并结合GenBank中红色原鸡的D-loop区序列,分析其线粒体D-loop区多态性。结果发现,2个原始地方品种鸡mtDNA D-loop区全长1 231~1 232 bp,在60只个体中共发现19处单核苷酸多态位点和8种单倍型。系统发育分析发现,8种单倍型可以分为A、B、D和E 4个分支,其中A和B为主要分支。A和B分支各有3种单倍型,D和E分支分别只有1种单倍型。A和B分支与红色原鸡滇南亚种(Gallus gallus spadiceus)聚为一类,E分支与红色原鸡印度亚种(Gallus gallus murghi)聚为一类,D分支与4个红色原鸡(Gallus gallus)亚种聚为一类。表明2个品种有多个母系起源,红色原鸡滇南亚种在2个原始鸡种形成过程中贡献最大。研究为家鸡的起源和遗传分化提供基础材料,同时也为2个品种的选育改良、遗传资源保护以及进一步的开发利用提供重要的理论依据.
Abstract: Chickens are an important animal genetic resource and the conservation of local breeds is an issue for preservation. With these highly diversified geographical environments, coupled with copious precipitation, adequate sunlight, and long frost-free periods, Yunnan Province has high biodiversity, including habitats for several ancestral species of domestic chicken and which has been suggested to be a center of domestication of chicken. 　　Daweishan mini and Chahua are typical local chicken breeds in Yunnan Province. This study evaluated the genetic diversity and origins of the two breeds by analyzing their mtDNA sequence polymorphisms. Blood samples were collected from 30 of each breed. Their complete D-loop sequences were PCR amplified, sequenced and compared with the DNA data of five red jungle fowl (Gallus gallus) subspecies annotated in the GenBank. 　　The results indicated that D-loop regions of the two local Yunnan chicken breeds were 1 231—1 232 bp, with a base C deficiency from 859 bp site in the 1 231 bp haplotype. Nineteen polymorphic sites were identified across the complete mtDNA D-loop region sequence. The eight haplotypes observed in the two local chicken breeds belonged to four previously published clades, i. e. Clades A, B, D and E. Of these clades, A and B were dominant. Clades A and B both contained three haplotypes, whereas Clades D and E both contained one haplotype. Clades A and B were clustered with G. g. spadiceus indicating that the two clades may originated from this subspecies. Clade E was clustered with G. g. murgh indicating that this clade may originated from this continental subspecies. The other haplotypes were contained in Clade D, which originated from multiple maternal origins. There were diversities in the middle of the mtDNA D-loop sequences of the two local breeds, which may result from multiple maternal origins. These data indicate that G. g. spadiceus contributed more to the evolution of the two local Yunnan breeds than the other four subspecies tested. The haplotypic diversity within the Daweishan mini chicken (0.685) and Chahua (0.476) was lower than those of breeds studied using the hypervariable I (HVI) segment of the D-loop in other chickens of Yunnan Province. 　　Determining the genetic background of domestic chicken breeds local to Yunnan Province and their relationships with red Jungle fowl could improve the understanding of chicken domestication. In contrast to other chickens of Yunnan Province, the two chicken breeds studied here had lower diversity at mitochondrial levels. Due to their possible contribution to past and recent domestication, the two breeds deserve a conservation attention.
出版日期: 2016-05-20
CLC:  S 831.2  
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樊艳凤
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高玉时
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贾晓旭
唐修君

引用本文:

陆俊贤, 贾晓旭, 唐修君, 樊艳凤, 唐梦君, 高玉时, 苏一军. 2个云南原始鸡种遗传多样性及其与红色原鸡的亲缘关系[J]. 浙江大学学报(农业与生命科学版), 2016, 42(3): 385-390.

LU Junxian, JIA Xiaoxu, TANG Xiujun, FAN Yanfeng, TANG Mengjun, GAO Yushi, SU Yijun. Genetic diversity of two local Yunnan chicken breeds and their relationships with red jungle fowl. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 42(3): 385-390.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2015.06.113    或    http://www.zjujournals.com/agr/CN/Y2016/V42/I3/385

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