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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2016, Vol. 42 Issue (2): 163-168    DOI: 10.3785/j.issn.1008-9209.2015.05.251
生物科学与技术     
大肠埃希菌感染后金银花尺蠖幼虫血细胞的变化
向玉勇*， 张云， 殷培峰， 朱萍
滁州学院生物与食品工程学院，安徽 滁州 239000
Variation of hemocytes of Heterolocha jinyinhuaphaga Chu larva infected by Escherichia coli.
XIANG Yuyong*, ZHANG Yun, YIN Peifeng, ZHU Ping
(School of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, Anhui, China)
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摘要： 为了探讨金银花尺蠖（Heterolocha jinyinhuaphaga Chu）幼虫血细胞的免疫反应，该研究观察了经不同浓度大肠埃希菌（1×103、1×104、1×105、1×106、1×107和1×108个/mL）感染后不同时间（3、6、12和24 h）金银花尺蠖幼虫血细胞形态、总数及各类血细胞比例的变化。结果表明：感染各浓度大肠埃希菌菌悬液后，金银花尺蠖部分血细胞发生变形、破裂、核移位以及胞质出现空泡等变化；在感染后各时间段内，血细胞总数较对照组均有显著增加，且随着时间延长血细胞总数增加迅速，12 h时达最高水平，24 h之后开始下降；3 h时原血细胞的比例明显减少，随着时间的延长，比例不断下降，12 h时降至最低水平，24 h之后开始回升；而浆血细胞、粒血细胞、珠血细胞、类绛血细胞、囊血细胞的数量相对增加，12 h时升至最高水平，24 h之后开始下降，浆血细胞、粒血细胞、囊血细胞在各时间段的比例差异有统计学意义（P0.05）。
Abstract: Hemocytes are the main executor of cellular immune system, which play an important role in defense against pathogen invasion. The immune defense mechanism has been concerned by many scholars. Many studies showed that hemocytes would contact pathogen through hemolymph circulation when encounter with invasion of pathogen, and pathogen would be devoured, formed nodules and agglutinated into tumor or be coated. However, in the immune response, pathogens would produce toxins to cause damage, resulting in variation on morphology, quantity and proportion of the hemocytes. Therefore, studying the immune function and mechanism of the hemocytes will provide theoretical reference for biological control of the pests. Heterolocha jinyinhuaphaga Chu was a newly discovered defoliator of honeysuckle, which was observed three generations per year in Anhui Province recently. The pest caused production reduction and even death of honeysuckle in a large scale, and brought great economic losses. Several studies have investigated the biological characteristics of the pest and its control measures, the immunity response of hemocytes has not been reported yet. In the preliminary study, six types of hemocytes including prohemocytes, plasmatocytes, granulocytes, spherulocytes, oenocytoids and cystocytes were found in H. jinyinhuaphaga Chu larva. In this study, the hemocytes of H. jinyinhuaphaga Chu infected by Escherichia coli were investigated, and the variation of morphology, total number and proportion of hemocytes in H. jinyinhuaphaga Chu larva at different time was observed, to understand the hemocyte types involved in the immunity response, to explore the mechanism of hemocyte immunity function and to provide scientific reference for developing effective microbial insecticides. The results showed that deformation, disruption, nucleus shift and vacuole were found in the hemocytes of H. jinyinhuaphaga Chu larva after infected by E. coli at different dosages. In each time period, the total number of hemocytes was significantly higher than control, and increased rapidly over time. The peak was observed at 12 h, and started to decrease in 24 h. The proportion of prohemocytes decreased significantly at 3 h, and declined over time, until the lowest level was observed at 12 h, and started to increase at 24 h. But the proportion of plasmatocytes, granulocytes, spherulocytes, oenocytoids and cystocytes relatively increased, and reached the highest level at 12 h, then began to decrease at 24 h. Significant difference was observed among the proportions of the plasmatocytes, granulocytes and cystocytes （P<0.05） at each time period, but not for the spherulocytes and oenocytoids （P<0.05）. In conclusion, infection by E. coli can cause hemocyte immune response in H. jinyinhuaphaga Chu, and the plasmatocytes, granulocytes and cystocytes are the main hemocytes involved in the immune response.
出版日期: 2016-03-20
CLC:  Q 969.93  
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向玉勇
张云
殷培峰
朱萍

引用本文:

向玉勇，张云，殷培峰，朱萍. 大肠埃希菌感染后金银花尺蠖幼虫血细胞的变化[J]. 浙江大学学报(农业与生命科学版), 2016, 42(2): 163-168.

XIANG Yuyong, ZHANG Yun, YIN Peifeng, ZHU Ping. Variation of hemocytes of Heterolocha jinyinhuaphaga Chu larva infected by Escherichia coli.. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 42(2): 163-168.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2015.05.251    或    http://www.zjujournals.com/agr/CN/Y2016/V42/I2/163

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