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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2015, Vol. 41 Issue (4): 414-420    DOI: 10.3785/j.issn.1008-9209.2015.04.071
生物科学与技术     
Gnαq基因在小鼠白色和黑色皮肤中的差异表达
尹志红，夏玉，赵新，王准，卢杰丽，庞全海
山西农业大学动物科技学院，山西 太谷 030801
Differential expression of Gnαq gene in white and black skin tissues of mice
Yin Zhihong, Xia Yu, Zhao Xin, Wang Zhun, Lu Jieli, Pang Quanhai
(College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi, China)
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摘要： 为了探讨鸟嘌呤核苷酸结合蛋白α亚基q(guanine nucleotide binding protein alpha q, Gnαq)基因与动物毛色形成的关系，采用反转录聚合酶链反应（reverse transcription-polymerase chain reaction, RT-PCR）、实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)技术和免疫印迹法(western blotting)检测Gnαq mRNA和其蛋白质的相对表达量；利用免疫组织化学法对Gnαq蛋白质在不同毛色皮肤组织中进行定位分析.RT-PCR结果显示：Gnαq的部分序列被扩增出，扩增片段长度为140 bp，经测序所得为目的片段。将所得基因序列在NCBI上与其他物种的序列进行同源性比对后发现，小鼠的Gnαq序列与其他物种的Gnαq序列有较高的同源性，尤其与大鼠、灰仓鼠的同源性最高，分别为98%和92%.qRT-PCR和免疫印迹结果显示：在黑色小鼠皮肤组织中Gnαq mRNA表达量和Gnαq蛋白质表达量均极显著高于白色小鼠皮肤组织(P<0.01).免疫组织化学法检测表明，Gnαq蛋白质在白色和黑色小鼠毛囊中的细胞周围表达，且在毛囊的外根鞘和毛球部有表达.总之，Gnαq在不同毛色皮肤组织中的表达量有一定的差异，且分布在毛囊的外根鞘和毛球部，说明Gnαq可能参与了小鼠被毛颜色的形成过程.
Abstract: Rich coat color is not only an important biological characteristics, but also a significant economic value for wool producing animals. Guanine nucleotide binding protein alpha q (Gnαq) is a downstream subunit of heterologous trimeric G protein. It plays a significant role in the performance of endothelin B receptor, which is involved in regulating coat hair of animals. 
In order to understand how Gnαq gene acts in the process of manipulating mammalian hair color, reverse transcriptionpolymerase chain reaction (RT-PCR), quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were used to detect the difference, the relative expression of Gnαq gene in different mice with two kinds of colors (white and black). The localizations of Gnαq protein were analyzed by immunohistochemistry in different color skin tissues of mice. 
A partial sequence of Gnαq gene with the length of 140 bp was successfully amplified by PCR. Blasting in NCBI showed that the Gnαq sequence of mice had a high homology with other species, especially with rat and gray dwarf hamster, with the similarity of 98% and 92%, respectively. qRT-PCR and western blotting results showed that the relative expression of Gnαq mRNA and Gnαq protein in black skin of mice was significantly higher than those in the white skin of mice (P<0.01). According to the results of immunohistochemistry, the Gnαq protein was expressed around the cells in hair follicles of mice, as well as in the outer root sheath and hair bulb of hair follicle.
In conclusion, the expression of Gnαq is significantly different between the white and black skin tissues of mice, and it is distributed in the outer root sheath and hair bulb of hair follicle, suggesting that the Gnαq gene may play a critical role in the process of coat color formation in mice.
出版日期: 2015-07-20
CLC:  Q 954.539  
通讯作者: 庞全海（http://orcid.org/0000-0002-5740-356X）     E-mail: pangquanhai@126.com
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尹志红
夏玉
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卢杰丽
庞全海

引用本文:

尹志红，夏玉，赵新，王准，卢杰丽，庞全海. Gnαq基因在小鼠白色和黑色皮肤中的差异表达[J]. 浙江大学学报(农业与生命科学版), 2015, 41(4): 414-420.

Yin Zhihong, Xia Yu, Zhao Xin, Wang Zhun, Lu Jieli, Pang Quanhai. Differential expression of Gnαq gene in white and black skin tissues of mice. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(4): 414-420.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2015.04.071    或    http://www.zjujournals.com/agr/CN/Y2015/V41/I4/414

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