Please wait a minute...
浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2015, Vol. 41 Issue (5): 593-601    DOI: 10.3785/j.issn.1008-9209.2015.03.062
动物科学     
甘草提取物对2,3,7,8-四氯二苯并-p-二噁英致建鲤肝损伤的影响
杜金梁, 曹丽萍, 贾睿, 刘英娟, 申玉金, 殷国俊
1.中国水产科学研究院淡水渔业研究中心,农业部淡水渔业和种质资源利用重点实验室,江苏 无锡214081;
2.中国水产科学研究院淡水渔业研究中心,农业部鱼类免疫药理学国际联合实验室,江苏 无锡214081;
3.南京农业大学无锡渔业学院,江苏 无锡214081
Hepatoprotective and antioxidant effects of Glycyrrhiza glabra extracts
 against TCDD-induced hepatocyte damage in Jian carp (Cyprinus carpio var.  Jian)
Du Jinliang, Cao Liping, Jia Rui, Liu Yingjuan, Shen Yujin, Yin Guojun
(1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi 214081, Jiangsu, China; 2. International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China; 3. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, Jiangsu,  China)
 全文: PDF(4592 KB)   HTML ( ) 
摘要： 为了评价甘草提取物是否对2,3,7,8-四氯二苯并-p-二噁英(2,3,7,8-tetrachlorodibenzo-p-dioxin,TCDD)引起建鲤肝组织损伤具有保护作用,本实验用含甘草提取物的饲料(0.1、0.5和1.0 g/kg)饲喂建鲤60 d,再腹腔注射0.6 μg/kg TCDD,72 h后收集血液和肝组织,检测血清和肝组织匀浆中谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(aspartate transaminase,AST)、乳酸脱氢酶(lactate dehydrogenase,LDH)、超氧化物歧化酶(superoxide dismutase,SOD)、碱性磷酸酶(alkaline phosphatase,AKP)、总蛋白(total protein,TP)、白蛋白(albumin,Alb)、过氧化氢酶(hydrogen peroxidase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GPx)、总抗氧化能力(total antioxidant capacity, T-AOC)、丙二醛(malondialdehyde,MDA)等生化指标,同时制备肝组织病理切片,观察甘草提取物对TCDD诱导建鲤肝组织损伤的影响。结果表明:1.0 g/kg甘草提取物显著降低血清中ALT、AST、LDH和AKP活性,显著提高总蛋白(TP)、白蛋白(Alb)质量浓度、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD)活性,降低丙二醛(MDA)质量摩尔浓度(P＜0.05或P＜0.01)。病理组织切片观察结果显示,甘草提取物处理组可以明显减轻TCDD引起肝组织损伤。提示,甘草提取物对TCDD引起的建鲤肝组织损伤具有较好的保护作用,而且甘草提取物的保护作用可能与其抗氧化性能有关。
Abstract: In recent years, the mechanism of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)  toxicity has  been a focal point in research. Generally it enriched in adipose tissue, and gradually amplified through food chains. It is seriously interfered with the normal function of the endocrine system of the organism, and displayed a wide spectrum of toxic effects, including dermal toxicity, reproductive toxicity, immunotoxicity and hepatotoxicity. The current research about the toxic mechanism of TCDD was mainly focused on the oxidative stress reaction, which was considered to be playing an important role in TCDD toxic research. Normally, the production and elimination of activated oxygen through the antioxidant system was in a balanceable and dynamic state. The antioxidant system could prevent excessive reactive oxygen species  causing oxidative stress, keep the body balance, maintain the normal structure and function of cells. TCDD combined with aromatic hydrocarbon receptor  and entered the nucleus, which changed the mRNA transcription of target gene, caused adverse change in the activity of enzymes and proteins, produced a series of oxidative stress reaction. Chinese medicinal herbs had been widely used in animal disease prevention due to their characteristics of rich bioactive components, free pollution, low side effects and immune enhancement. Many studies had reported that natural antioxidants were efficacious in preventing oxidative stress-related liver pathologies due to particular interactions and synergisms. Glycyrrhiza glabra extracts (GGE) was one of the common traditional Chinese herbal medicine, it could adjust the immunity, and had anti-tumor, anti-virus, antioxidant effects and so on. Although numerous studies had reported its beneficial effects on mammals, there was a lack of reports on its function in aquatic animals. The aim of this study was to  evaluate the hepatoprotective and antioxidant effects of GGE on the TCDD-induced liver injury in Jian carp (Cyprinus carpio var. Jian). Jian carp were fed with diet containing 3 doses of GGE (0.1, 0.5 and 1.0 g/kg diet) for 60 days before a single intraperitoneal injection of TCDD (0.6 μg/kg), 72 h after TCDD injection, blood and liver samples were taken for biochemical analysis. The results showed that GGE at 1.0 g/kg diet for 60 days prior to TCDD intoxication significantly reduced the elevated activities of ALT, AST, LDH, AKP and increased the reduced levels of total protein (TP), albumin (Alb) in the serum, markedly inhibited the reduction of the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), total antioxidant capacity (T-AOC), and reduced the malondialdehyde (MDA) formation in liver tissue. Meanwhile, histopathological observation also showed that GGE can significantly relieve TCDD induced liver injury. It is concluded that GGE exhibited protective effect against TCDD-induced hepatotoxicity in fish, which is likely related to its antioxidant activity, and it is suggested that GGE  may potentially be used as a hepatoprotective agent for fish liver injury.
出版日期: 2015-09-20
CLC:  X 503.225  
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　
杜金梁
曹丽萍
贾睿
刘英娟
申玉金
殷国俊

引用本文:

杜金梁, 曹丽萍, 贾睿, 刘英娟, 申玉金, 殷国俊. 甘草提取物对2,3,7,8-四氯二苯并-p-二噁英致建鲤肝损伤的影响[J]. 浙江大学学报(农业与生命科学版), 2015, 41(5): 593-601.

Du Jinliang, Cao Liping, Jia Rui, Liu Yingjuan, Shen Yujin, Yin Guojun. Hepatoprotective and antioxidant effects of Glycyrrhiza glabra extracts
 against TCDD-induced hepatocyte damage in Jian carp (Cyprinus carpio var.  Jian). Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(5): 593-601.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2015.03.062    或    http://www.zjujournals.com/agr/CN/Y2015/V41/I5/593

No related articles found!