Please wait a minute...
浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2015, Vol. 41 Issue (2): 237-244    DOI: 10.3785/j.issn.1008-9209.2014.04.061
动物科学     
猪神经元蛋白3.1基因3′侧翼区多聚腺苷酸数目多态性及其与肉质性状的关系
杨云, 曾勇庆*, 张哲, 徐正刚, 陈伟, 房国锋, 王守栋
山东农业大学动物科技学院,动物遗传育种学实验室,山东 泰安 271018
PolyA polymorphisms in 3′ flanking region of pig neuronal protein 3.1 gene and their relationships with meat traits.
Yang Yun, Zeng Yongqing*, Zhang Zhe, Xu Zhenggang, Chen Wei, Fang Guofeng, Wang Shoudong
(Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, Shandong, China)
 全文: PDF(3312 KB)   HTML ( ) 
摘要： 选取54头莱芜猪和40头鲁莱黑猪,宰后测定肉质性状,利用聚合酶链反应及单链构象多态性（polymerase chain reaction-single strand conformation polymorphism, PCR-SSCP）技术检测其神经元蛋白3.1（neuronal protein 3.1, P311）基因3′侧翼区2个多聚腺苷酸（polyA）结构的长度多态性,测序确定连续腺苷酸的数目,并与肉质性状进行关联分析，以期发现有效多态位点.结果表明：1）在莱芜猪和鲁莱黑猪中共检测出2个连续腺苷酸数目的多态位点polyA-L1和polyA-L2,两者的polyA数目分别为18、15和15、12,2个位点的3种基因型分别记为polyA-L1位点的MM型、NN型和MN型,以及polyA-L2位点的BB型、DD型和BD型；这2个多态位点在莱芜猪和鲁莱黑猪群体中均处于哈代温伯格平衡（P>0.05）,其多态性在2个猪种间的分布差异在统计学上均不显著.2）莱芜猪polyA-L1位点的3种基因型之间在肉质性状上均未表现出统计学上的显著差异,但MN型个体各项肉质性状普遍优于2个纯合型,肉质较好,鲁莱黑猪中的结果与此相同.3）莱芜猪polyA-L2位点的3种基因型之间在肉质性状上均未表现出统计学上的显著差异,但鲁莱黑猪在该位点处的失水率和烹饪损失指标在统计学上差异显著,其中DD型个体的失水率显著高于BD型个体,BB型和DD型个体的烹饪损失显著高于BD型,其余各指标的基因型间在统计学上差异不显著.总之,在这2个位点处普遍表现为杂合型的肉质性状优于纯合型,生产中应重视2个纯合型个体间的杂交利用,以产生更多肉质优良的杂合型个体.
Abstract: The neuronal protein 3.1 （P311）, also called PTZ17, is coded by the P311 gene, which is located in the porcine chromosome 2. The P311 is extensively distributed in the nervous and muscular systems and had the functions in myofibroblast differentiation and alveolar generation processes. It is also closely related to the fat deposition. The 3′ flanking region doesnt code for protein, but it can affect the phenotype by some ways, such as the regulation of the degradation and translation rates of the mRNA. The research on the polymorphisms of the 3′ flanking region in the porcine P311 gene is still rare. The aim of the study was to identify the length polymorphisms of the 3′ flanking region in the porcine P311 gene and to conduct association analysis between the polymorphisms and the meat traits in order to find some efficient polymorphic loci for the breeding of high quality pigs with good meat traits. In this research, 54 Laiwu pigs and 40 Lulai pigs were chosen and slaughtered. The ear and Longissimus dorsi samples were collected for DNA extraction and meat traits determination. The extracted DNA was amplified by polymerase chain reaction （PCR） and the products were used to detect the polymorphic type with the method of single strand conformation polymorphism （SSCP） and sequencing. The meat traits were estimated in nine aspects including water loss, cooking loss, tenderness, pH1, pHμ, meat color score, marbling score, drip loss and intramuscular fat (IMF) content. All the data collected were analyzed using the SPSS 13.0 and R software. The results showed that there were both two kinds of polyA numbers in the two loci of polyA-L1 and polyA-L2. The polyA numbers in the polyA-L1 and polyA-L2 loci were 18, 15 and 15, 12, respectively, and the genotypes were termed as MM, NN, MN in the polyA-L1 locus and BB, DD, BD in the polyA-L2 locus. The preponderant allele in the polyA-L1 locus in both of the two breeds was M allele, and the genotype frequency was MM>MN>NN. In the polyA-L2 locus, the preponderant allele in the Laiwu pig was B allele and the genotype frequency was BD>BB>DD, but in the Lulai pig, the gene frequencies of the two alleles were equal and the genotype frequency was BD>BB=DD. The heterozygosity index showed that the heterozygosity of the polyA-L2 locus in the two breeds was higher than that of the polyA-L1 locus. The results of chi-square test demonstrated that the two polymorphic loci in the Laiwu and Lulai populations both fitted the Hardy-Weinberg equilibrium situation （P>0.05）. The polymorphic distributions between the two breeds showed no statistically significant differences at the two loci. In the polyA-L1 locus of the two breeds, none of the nine meat traits differed significantly among the three genotypes of MM, MN and NN, but the data displayed a trend that the performances of the MN genotype were better than those of the MM and NN genotypes. The IMF content, pH1 and marbling score of the MN genotype were the highest and its drip loss and cooking loss were the lowest within the three genotypes. At the same time, the shear force value of MN genotype was lower than that of MM genotype. Among the three polymorphic types of BB, BD and DD in the polyA-L2 locus, significant differences were found in water loss and cooking loss in the Lulai population （P<0.05）. The performances of the heterozygote were lower than those of the two homozygotes with no significant differences detected in other conditions. In the Laiwu population, the performances of DD genotype were the worst among the three genotypes, for its IMF, pH1 and pHμ were the lowest while the cooking loss and drip loss were the highest. In the Lulai population, the BD genotype performed the best for its highest IMF, pH1, pHμ and marbling score, and the lowest cooking loss and water loss. It is concluded that the two breeds with different genotypes in the two loci show different meat traits and the heterozygotes display better than the homozygotes in some indexes, such as cooking loss, drip loss, marbling score and IMF content. As a result, hybridization should be widely used in practice to obtain the ideal heterozygotes with better meat traits.
出版日期: 2015-03-20
CLC:  S 828.8  
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　
房国锋
王守栋
杨云
曾勇庆
张哲
徐正刚
陈伟

引用本文:

杨云, 曾勇庆, 张哲, 徐正刚, 陈伟, 房国锋, 王守栋. 猪神经元蛋白3.1基因3′侧翼区多聚腺苷酸数目多态性及其与肉质性状的关系[J]. 浙江大学学报(农业与生命科学版), 2015, 41(2): 237-244.

Yang Yun, Zeng Yongqing, Zhang Zhe, Xu Zhenggang, Chen Wei, Fang Guofeng, Wang Shoudong . PolyA polymorphisms in 3′ flanking region of pig neuronal protein 3.1 gene and their relationships with meat traits.. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(2): 237-244.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2014.04.061    或    http://www.zjujournals.com/agr/CN/Y2015/V41/I2/237

null
No related articles found!