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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2014, Vol. 40 Issue (6): 647-652    DOI: 10.3785/j.issn.1008-9209.2014.01.201
农业科学     
茶树中过氧化氢酶活力变化规律
杨节， 龚淑英*， 唐德松
（浙江大学农业与生物技术学院茶学系，杭州 310058）
Regulation of catalase activity variation of tea tree (Camellia sinensis).
Yang Jie, Gong Shuying*, Tang Desong
(Department of Tea Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China)
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摘要： 用紫外分光光度法测定茶树鲜叶的过氧化氢酶（catalase，CAT）活力，研究茶树CAT活力变化规律.结果表明：相同地点种植的不同茶树品种在生长旺盛的春季，其新梢的酶活力虽然会有一定的差异，但总体上差异不明显，到了夏季各品种间的差异较大，秋季酶活力普遍较高，但差异减小；不同季节、同一品种的茶树CAT活力变化明显，总体表现为秋季>夏季>春季；生长在主干的鲜叶CAT活力高于新生枝条上的鲜叶，而在同一枝条上，位于枝条中间的鲜叶CAT活力高于位于枝条两端的鲜叶；刚采下来的茶树鲜叶在摊放2 h后酶活力上升30%，而随着摊放时间的进一步延长，CAT活力持续下降，到12 h后酶活力基本稳定在100 U/g左右.可见，茶树CAT活力变化对茶树正常生长发育起着极为重要的作用，是茶树逆境生理的关键酶.
Abstract: Catalase (CAT) is a protective enzyme responsible for the degradation of hydrogen peroxide before it damages the cellular components. All aerobic organisms and many anaerobic organisms possess it in virtually. The catalatic reaction takes place in two steps: The first hydrogen peroxide molecule oxidizes the heme to an oxyferryl species in which one oxidation equivalent is removed from the iron and one from the porphyrin ring to generate a porphyrin cation radical; the second hydrogen peroxide is then used as a reductant of compound 1 to regenerate the resting state enzyme, water, and oxygen. But so far the reports about the CAT in tea tree (Camellia sinensis) are not seen so often. In this research, the regulation of CAT activity variation of tea tree was studied. The CAT was extracted from fresh tea leaves with 0.2 mol/L phosphate buffer (pH 7.4) and 15% polyvinylpolypyrrolidone (PVPP). The CAT activity was measured spectrophotometrically at 240 nm. One unit of CAT activity was defined as the activity required to destroy 1 μmol H2O2 at 25 ℃ in 0.2 mol/L phosphate buffer for 1 min. The results about the regulations of tea CAT activity variation showed that, different cultivars of tea tree had different CAT activities, especially in summer. And the CAT activity varied when the season changed, and the activity variation tendency was as autumn > summer > spring. The leaves located in the older branch had a higher CAT activity than those in the new branch; the leaves in vigorous growth had a higher CAT activity than the new and old leaves. When spreading for 2 h, the CAT activity of tea leaves increased, as the spreading time became longer, the CAT activity decreased until 100 U/g and hold for a long time. It is concluded that the CAT is one of the key enzymes affected tea tree growth and development. In different growth and development stages, the CAT activity is different in tea tree. The basic regulation is that the CAT activity in tea tree increases under stress but decreases when the stress is extremely severe.
出版日期: 2014-11-20
CLC:  S 571.1  
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杨节
龚淑英*
唐德松

引用本文:

杨节， 龚淑英*， 唐德松. 茶树中过氧化氢酶活力变化规律[J]. 浙江大学学报(农业与生命科学版), 2014, 40(6): 647-652.

Yang Jie, Gong Shuying*, Tang Desong. Regulation of catalase activity variation of tea tree (Camellia sinensis).. Journal of Zhejiang University (Agriculture and Life Sciences), 2014, 40(6): 647-652.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2014.01.201    或    http://www.zjujournals.com/agr/CN/Y2014/V40/I6/647

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