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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2014, Vol. 40 Issue (5): 495-504    DOI: 10.3785/j.issn.1008-9209.2013.11.141
农业科学     
光照及氮素水平对水稻冠层叶片SPAD值动态变化的影响
何俊俊, 杨京平*, 杨虎, 赵杏, 叶欣怡
（浙江大学环境与资源学院环境保护研究所,杭州310058）
Effects of light intensity and nitrogen supply on the dynamic characteristics of leaf SPAD value of rice canopy
He Junjun, Yang Jingping*, Yang Hu, Zhao Xing, Ye Xinyi
(
Institute of Environmental Science and Technology, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China
)
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摘要： 在盆栽试验条件下,研究在3个遮阴水平和5个施氮水平处理组合下,水稻分蘖期、孕穗期和抽穗期冠层叶片叶绿素计测定值（soil and plant analyzer  development value,
SPAD value）的空间分布规律及其差异,为弱光条件下实时进行水稻叶片氮素营养诊断及优化提供依据。结果表明,同对照相比,在遮阴条件下水稻冠层SPAD值波动幅度受到明显抑制。遮光使水稻冠层叶片SPAD值下降,并抑制施氮水平对水稻叶片向上位叶输送氮素的正向作用。经变异系数分析发现,L4叶对施氮水平变化反应最为敏感。另外,在中度和重度遮阴条件（遮光率分别为65%和85%）下可利用叶片相对叶色差值（relative SPAD value,RSPAD）进行氮素营养状态的诊断。相对叶色差值SPADL3-L2，SPADL4-L3与施氮水平呈显著线性正相关关系（R2L3-L2=0.87～0.97,R2L4-L3=0.85～0.97）,这一关系不受生育期和施氮水平的影响。在正常光照条件下,宜选用SPADL3-L2指示水稻整个生育期氮素营养状况。在日照较弱的季节或受林木遮光影响较大的田块,宜选择SPADL4-L3作为诊断指标。
Abstract: The application of real-time, site-specific nitrogen management can guarantee the high yield of rice, and improve the utilization rate of nitrogen. The management pointed out that soil and plant analyzer  development value （SPAD value） could be an index for nitrogen application precise precision, namely using chlorophyll meter as a nitrogen nutrition diagnosis tools on rice. Previous studies had looked at the effects of cultivars, growth stage, leaf position and other factors on SPAD value, while research on the influence of light and nitrogen on rice canopy SPAD value is few in our country. Therefore, it is necessary to study the distribution characteristics of SPAD value under low light conditions. And  the feasibility of applying chlorophyll meter on nitrogen nutrition diagnosis was investigated. This article studied the methods to optimize nitrogen nutrition diagnosis of rice leaves under low-light stress condition with SPAD meter. Pot experiments were conducted to study the distributing characteristics of canopy SPAD and the distributional difference at tillering, booting and heading stages. There were three levels of shading and five levels of nitrogen supply in the experiment.
There exists a law that the greenness of rice leaf was regularly changed as black-yellow alternation ways through the whole growth period of rice despite of the effect of light deficiency. But the fluctuation was significantly suppressed under shading conditions. The decrease of SPAD value resulted from lacking light. Improving nitrogen level had positive effect on increasing the SPAD values of functional leaves. However, less-light condition made an inhibitory effect on the positive role of nitrogen. Shading treatment promoted the increment of lower leaf SPAD values. The fourth leaf (L4) has an instruction function for nitrogen nutrition status during the key growing periods under the interaction of light density and nitrogen supply, and it may be a better leaf indicator for rice N status. SPADL3-L2 and SPADL4-L3 (relative SPAD value, RSPAD) were remarkably correlated linearly with nitrogen level (under full sunlight, R2L3-L2=0.870.97; under shading treatment, R2L4-L3=0.850.97) without considering the influence from the growing stages and nitrogen rates. For Hang 43, SPAD values of the top four leaves on main stem could be used as an indicator for diagnosis of nitrogen status and nitrogenous fertilizer application in rice under shading. Under the natural ultraviolet radiation condition, SPADL3-L2 will be a better one for nutrition diagnosis. When light intensity is low and less (deficient sunshine or light attenuation by mutual shading during the growing period of rice), SPADL4-L3 may perform better for N status diagnosis.
 
出版日期: 2014-09-20
CLC:  S 143.1  
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杨虎
赵杏
叶欣怡
何俊俊
杨京平*

引用本文:

何俊俊, 杨京平*, 杨虎, 赵杏, 叶欣怡. 光照及氮素水平对水稻冠层叶片SPAD值动态变化的影响[J]. 浙江大学学报(农业与生命科学版), 2014, 40(5): 495-504.

He Junjun, Yang Jingping*, Yang Hu, Zhao Xing, Ye Xinyi . Effects of light intensity and nitrogen supply on the dynamic characteristics of leaf SPAD value of rice canopy. Journal of Zhejiang University (Agriculture and Life Sciences), 2014, 40(5): 495-504.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2013.11.141    或    http://www.zjujournals.com/agr/CN/Y2014/V40/I5/495

[1] 刘彦伶1,2， 来庆3， 徐旱增4， 张宣1,2,吴良欢1,2*， 顾艳1,2， 何艳1,2， 孔向军5. 不同氮肥类型对黄泥田双季稻产量及氮素利用的影响[J]. 浙江大学学报(农业与生命科学版), 2013, 39(04): 403-412.