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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2014, Vol. 40 Issue (3): 266-    DOI: 10.3785/j.issn.1008-9209.2013.10.302
生物科学与技术     
外源水杨酸对低温胁迫下蝴蝶兰的缓解效应及其抗氧化生理特征变化
陈丹1, 王丹1, 孙丽1, 张艳嫣1, 黄冲平1,2*
(1.浙江大学农业与生物技术学院农学系,杭州 310058;2.浙江大学农业试验站,杭州310058)
Alleviating effects of exogenous salicylic acid on antioxidative physiological characters of Phalaenopsis under low temperature stress.
Chen Dan1, Wang Dan1, Sun Li1, Zhang Yanyan1, Huang Chongping1,2*
(
1. Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2.Agricultural Experiment Station, Zhejiang University, Hangzhou 310058, China
)
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摘要： 以5个不同品种的蝴蝶兰为材料,分别于叶面喷施不同浓度(0,0.2,0.4,0.6,0.8 mmol/L)的水杨酸(salicylic acid, SA)溶液,将其置于人工智能温室昼夜温度11 ℃/6 ℃的生长箱内低温胁迫处理3 d和常温(27 ℃/22 ℃)恢复生长7 d,研究蝴蝶兰叶片抗氧化系统和光合系统的生理特征变化。结果表明:外源水杨酸对低温胁迫下蝴蝶兰叶片的伤害具有缓解效应,以0.4~0.6 mmol/L的处理效果最佳,提高了蝴蝶兰叶片中的叶绿素、抗坏血酸(ascorbic acid, AsA)和谷胱甘肽(glutathione, GSH)质量分数以及Fv/Fm和超氧化物歧化酶(superoxide dismutase, SOD)活性,同时降低了抗坏血酸过氧化物酶 (ascorbate peroxidase, APX)活性和可溶性糖质量分数。低温胁迫解除后,水杨酸处理能促进叶绿素质量分数的上升和Fv/Fm的恢复;提高了SOD酶活性和GSH的积累;降低了APX的活性以及AsA质量分数,促进细胞内光合系统和抗氧化系统的恢复。比较不同耐冷性的蝴蝶兰品种表明,耐冷性强的“婚宴”对水杨酸处理更为敏感,效果更为显著。
Abstract: Phalaenopsis spp. is one of the most important orchids, which has a great economic value as potted plants in China. However, growth disorders like damage to membranes, generation of reactive oxygen species (ROS), protein denaturation, and accumulation of toxic compounds due to low temperature stress have become one of the key constraints for Phalaenopsis consumption during winter season in north subtropical area of China. There are a large number of  reports about using salicylic acid (SA) to alleviate the negative effects of chilling stress on plants.  However there is rare report about the changes of antioxidant systems under chilling stress with exogenous plant regulators application such as SA in Phalaenopsis. The objectives of this study are  to investigate the alleviating effects of exogenous SA application on Phalaenopsis under low temperature stress and in recovery growth, to determine the changes of antioxidative enzyme activities and key indicators of photosynthetic system. Five different cultivars of Phalaenopsis (“Hunyan” “Dalajiao” “Tianjiao” “Tianxianggongzhu” and “V31”) were foliar sprayed with a series of SA concentrations (0,0.2,0.4,0.6,0.8 mmol/L). After foliar application, the plants were transferred to a growth chamber treated at low temperature (LT) of 11  ℃ /6  ℃ (day/night) for 3 days and then to recover growth at regular temperature 27  ℃ /22  ℃ (day/night) for 7 days. The changes of antioxidant system and photosynthetic system in Phalaenopsis leaves were tested. The results showed that the foliar spray of SA could obviously alleviate the negative effects of LT stress. In this study, the best concentration of SA was from 0.4 to 0.6 mmol/L. Appropriate concentrations of SA could significantly increase the chlorophyll content, Fv/Fm, superoxide dismutase (SOD) activity, ascorbic acid (AsA) and glutathione (GSH) contents and improve the chilling tolerance of Phalaenopsis. The activity of ascorbate peroxidase (APX) and soluble sugar content  were decreased in most of LT stress treatments. After LT stress, exogenous SA could promote the plant growth. After 7 days recovery growth, application of 0.6 mmol/L SA increased SOD and APX activities, chlorophyll content and Fv/Fm, and decreased AsA and GSH contents. The treatment enhanced the photosynthetic and antioxidant capacity of the plants. These results indicated that the application of appropriate concentration of SA can improve the LT tolerance of Phalaenopsis. Compared the different varieties of Phalaenopsis, it is concluded that the variety “Huanyan” is of strong LT tolerance and more sensitive and effective to SA application.
出版日期: 2014-05-20
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黄冲平1
2*
陈丹1
王丹1
孙丽1
张艳嫣1

引用本文:

陈丹1, 王丹1, 孙丽1, 张艳嫣1, 黄冲平1,2*. 外源水杨酸对低温胁迫下蝴蝶兰的缓解效应及其抗氧化生理特征变化[J]. 浙江大学学报(农业与生命科学版), 2014, 40(3): 266-.

Chen Dan1, Wang Dan1, Sun Li1, Zhang Yanyan1, Huang Chongping1,2*. Alleviating effects of exogenous salicylic acid on antioxidative physiological characters of Phalaenopsis under low temperature stress.. Journal of Zhejiang University (Agriculture and Life Sciences), 2014, 40(3): 266-.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2013.10.302    或    http://www.zjujournals.com/agr/CN/Y2014/V40/I3/266

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