Please wait a minute...
浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2014, Vol. 40 Issue (2): 146-154    DOI: 10.3785/j.issn.1008-9209.2013.09.111
生物科学与技术     
拟松材线虫携带细菌对云南松的致病性
李姝江1， 朱天辉1*， 张兴华1,2
（1.四川农业大学林学院，四川 雅安 625014；2.金堂县林业园林管理局，四川 金堂 610400）
Pathogenicity of bacterium carried by Bursaphelenchus mucronatus on Pinus yunnanensis.
Li Shujiang1, Zhu Tianhui1*, Zhang Xinghua1,2
(1. College of Forestry, Sichuan Agricultural University, Ya’an, Sichuan 625014, China; 2. Jintang County Administration Bureau of Forestry and Gardens, Jintang, Sichuan 610400, China)
 全文: PDF(1164 KB)   HTML ( ) 
摘要： 为探讨拟松材线虫(Bursaphelenchus mucronatus)携带细菌在对云南松(Pinus yunnanensis)致病过程中的作用，选用二年生云南松苗木、从四川省松材线虫病病区分离到的拟松材线虫、线虫携带的细菌及其代谢产物为材料，通过室内对云南松枝条韧皮部滴管和松针浸渍法接种，观察并计算松针枯黄率，测定不同处理时间后松针的总酚含量和苯丙氨酸解氨酶（phenylalanine ammonialyase，PAL）、多酚氧化酶（polyphenol oxidase，PPO）、过氧化物酶（peroxidase，POD）等的酶活性变化来表征细菌及其代谢产物对云南松的致病力.结果表明，从拟松材线虫体表分离的26种细菌中，枯草芽孢杆菌Ⅲ(Bacillus subtilis Ⅲ)、嗜麦芽寡养单胞菌Ⅰ(Stenotrophomonas maltophilia Ⅰ)、杓兰果胶杆菌(Pectobacterium cypripedii)、解淀粉芽孢杆菌Ⅰ(B. amyloliquefaciens Ⅰ)、枯草芽孢杆菌Ⅴ(B. subtilis Ⅴ)所致段斑长度属于最高值范围且差异均无统计学意义.在进一步盆栽试验中仅杓兰果胶杆菌致病作用明显，其中，无菌拟松材线虫+杓兰果胶杆菌和无菌拟松材线虫+杓兰果胶杆菌非蛋白代谢产物的混合处理对云南松针叶致病性最强，但两者间差异无统计学意义，而与其余各处理间差异有统计学意义；在对云南松松针苯丙烷类代谢的影响中，灭菌线虫+杓兰果胶杆菌非蛋白代谢产物的混合处理作用最强，可以导致松针总酚含量，酚类抗氧化产物POD、PPO、PAL活性不断减少，且各指标与枯黄率显著相关.由此可知，云南松拟松材线虫病的发生、发展与寄主苯丙烷类代谢的异常变化有关.
Abstract: Pine wood nematode (PWN) is a destructive disease to pines. For the research on its pathogenic mechanism, the theory which is about the complex infection of PWN and the bacterium carried by it is recognized. Bursaphelenchus mucronatus is considered as a related species of Bursaphelenchus xylophilus, which may be differentiation with the homologous species in different environments. In early studies, B. mucronatus was served as contrast species to B. xylophilus due to the weak or lacking pathogenicity. But recently, many reports have pointed out that it has the strong pathogenicity and can cause the widespread death of pines, which referred to Pinus thunbergii, P. yunnanensis, P. taiwanensis, P. densata, etc. The large numbers of P. yunnanensis were dead in Sichuan in recent years. However, no systematic study was conducted on the pathogenicity of the bacterium carried by B. mucronatus. This study has the aim of evaluating the effects of the bacterium carried by B. mucronatus on wilting ratio and phenylpropanoid metabolism of pine needles, and further exploring the effect of bacterium in the pathogenic process of B. mucronatus. Two-year-old P. yunnanensis seedlings, B. mucronatus isolated from PWN disease district in Sichuan, the bacterium carried by B. mucronatus and its metabolite as the materials, the wilting ratio was calculated, and the total phenol content, phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD) activities were determined by the inoculation of burette to branch phloem of P. yunnanensis in laboratory, which represented the pathogenicity of the bacterium and its metabolite on P. yunnanensis. The results showed that Pectobacterium cypripedii and its non-protein metabolite had strongest effect on P. yunnanensis in 26 species of bacteria isolated from the surface of nematodes, and the wilting degree and wilting ratio were significantly high in these two treatments. They caused the accumulation of total phenol content and the changes of PAL, PPO and POD activities, the PPO and POD activities of which increased from 12 h to 24 h and declined after 24 h, and PAL activities declined during 12-48 h. Moreover, in above each index, significant correlation with wilting ratio was observed, but among the indexes in phenylpropanoid metabolism, only PAL and PPO activities were found to have a significant correlation. It is concluded that P. cypripedii and its nonprotein metabolite can continuously decrease the phenolic antioxidants, which makes the metabolism to be disordered and induce cell toxicity of the needles through affecting the phenylpropanoid metabolism. And then, the needles display lesion, fading, browning, drying and even death. The above results reveal that the occurrence and development of PWN disease is closely related to the abnormal changes of the phenylpropanoid metabolism in host.
出版日期: 2014-03-20
CLC:  S 763  
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　
2
李姝江1
朱天辉1*
张兴华1

引用本文:

李姝江1， 朱天辉1*， 张兴华1,2. 拟松材线虫携带细菌对云南松的致病性[J]. 浙江大学学报(农业与生命科学版), 2014, 40(2): 146-154.

Li Shujiang1, Zhu Tianhui1*, Zhang Xinghua1,2. Pathogenicity of bacterium carried by Bursaphelenchus mucronatus on Pinus yunnanensis.. Journal of Zhejiang University (Agriculture and Life Sciences), 2014, 40(2): 146-154.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2013.09.111    或    http://www.zjujournals.com/agr/CN/Y2014/V40/I2/146

No related articles found!