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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2014, Vol. 40 Issue (2): 223-230    DOI: 10.3785/j.issn.1008-9209.2013.09.031
动物科学     
在低盐度胁迫下鲻幼鱼鳃丝Na+-K+-ATP酶活性及其β亚基mRNA表达与显微结构变化
李磊1， 蒋玫1， 王云龙1， 吴庆元2， 牛俊翔2， 沈新强1*
(1.中国水产科学研究院东海水产研究所，上海 200090；2.上海海洋大学海洋科学学院，上海 201306)
Effects of low salinity stress on Na+-K+-ATPase activities, expression of Na+-K+-ATPase β-subunit mRNA and microscopical structure in gill filaments of juvenile Mugil cephalus.
Li Lei1, Jiang Mei1, Wang Yunlong1, Wu Qingyuan2, Niu Junxiang2, Shen Xinqiang1*
(1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; 2. College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China)
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摘要： 探讨在低盐度胁迫下鲻(Mugil cephalus)幼鱼鳃丝Na+-K+-ATP酶活性、Na+-K+-ATP酶β亚基mRNA表达量变化以及对鳃丝组织显微结构的影响.结果表明：在盐度分别为20(对照组)，15，10，5和0实验条件下，20 d后各低盐度组鳃丝Na+-K+-ATP酶活性均高于对照组；0和5盐度组鳃丝Na+-K+-ATP酶活性在实验过程中表现为持续上升的趋势，前期升高幅度大，后期升高幅度减小；10和15盐度组鳃丝Na+-K+-ATP酶活性在15 d后达到峰值，至20 d呈现小幅下降趋势.鳃丝Na+-K+-ATP酶β亚基mRNA表达的变化趋势与Na+-K+-ATP酶活性的变化趋势基本保持同步，显示出了其对Na+-K+-ATP酶活性的调控作用.对鳃丝显微结构观察的结果显示，随着盐度的降低，鲻幼鱼鳃小片逐渐变宽，伴随间距缩小，同时泌氯细胞数量逐渐减少，体积缩小，扁平细胞体积增大，黏液细胞数量增多.这表明在低盐度胁迫下鲻幼鱼鳃的生理功能和组织结构具有随外界水体盐度变化的适应能力.
Abstract: In natural aquatic system, salinity can largely influence growth, breeding, and even fishes survival. In general, euryhaline fishes often possess strong osmotic adjustment capacity. The gill filaments play an important role in the maintenance of blood ion and acid-base balance in seawater-acclimated fishes, especially if there is a sudden change in water salinity. Seawater-acclimated fishes drink seawater to counter osmotic loss of water, and secrete excess Na+ and Cl- across the gill filaments. When there is a change between freshwater and seawater, euryhaline fishes must transform their gill filaments from an ionabsorbing epithelium to an ionsecreting epithelium, or vice versa. Associated with this transformation is change of Na+/K+-ATPase activity, Na+/K+-ATPase gene expression and structural change of the gill. Euryhaline teleost fishes, such as killifish Fundulus heteroclitus and Mozambique tilapia Oreochromis mossambicus, have been used as model animals for the study of osmo- and ion-regulation in teleost fish. However, there are little accumulating researches and available literature on juvenile Mugil cephalus. Thus, the objective of the present study is to explore the response and adjustment mechanisms of juvenile M. cephalus to salinity stress. The present study examined the effects of low salinity stress (salinity 15, 10, 5, 0, and salinity 20 as control) on Na+-K+-ATPase activities, expression of Na+-K+-ATPase β-subunit mRNA and structural changes in the gill filaments of juvenile M. cephalus during the experimental period of 20 days. The results showed that no fish mortality occurred for all experimental groups during the study period. The Na+-K+-ATPase activities in the gill filament tissue were significantly lower in the control group than in the treatment groups. At the low salinity groups (0 and 5), the Na+-K+-ATPase activities showed rising trends by high and low amplitude during the early and later experimental period, respectively, and the activities at salinity 10 and 15 groups attained the maximum value in 15 days, and then slightly decreased. The expression of Na+-K+-ATPase β-subunit mRNA changed according with the Na+-K+-ATPase activity. It showed rising trend with the decrease of salinity. There were significant differences in Na+-K+-ATPases and their β-subunit mRNA expressions between the control group and salinity 0 group from day 5 to day 20, as well as between the control group and salinity 5 group from day 10 to day 20 (P<0.05). The highest expression of Na+-K+-ATPase β-subunit mRNA at the salinity 0, 5 groups was 4.10 and 2.51 times higher than that at the control group respectively, suggesting that the Na+-K+-ATPase β-subunit mRNA played an important role in the process of regulation. With the decrease of salinity, the gill lamellae gradually became wide, and the distance between them as well as the number and size of chloride cells showed decreasing trend, while the size of pavement cells and the number of mucous cells consistently increased. In conclusion, the structure and physiological functions of the gill of juvenile M. cephalus adapt themselves to the salinity change.
出版日期: 2014-03-20
CLC:  S 965.2  
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李磊1
蒋玫1
王云龙1
吴庆元2
牛俊翔2
沈新强1*

引用本文:

李磊1， 蒋玫1， 王云龙1， 吴庆元2， 牛俊翔2， 沈新强1*. 在低盐度胁迫下鲻幼鱼鳃丝Na+-K+-ATP酶活性及其β亚基mRNA表达与显微结构变化[J]. 浙江大学学报(农业与生命科学版), 2014, 40(2): 223-230.

Li Lei1, Jiang Mei1, Wang Yunlong1, Wu Qingyuan2, Niu Junxiang2, Shen Xinqiang1*. Effects of low salinity stress on Na+-K+-ATPase activities, expression of Na+-K+-ATPase β-subunit mRNA and microscopical structure in gill filaments of juvenile Mugil cephalus.. Journal of Zhejiang University (Agriculture and Life Sciences), 2014, 40(2): 223-230.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2013.09.031    或    http://www.zjujournals.com/agr/CN/Y2014/V40/I2/223

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