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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2014, Vol. 40 Issue (2): 155-164    DOI: 10.3785/j.issn.1008-9209.2013.09.021
农业科学     
西藏野生大麦与栽培大麦氮利用效率的基因型差异(英文)
杨丽娜， 胡宏亮， 朱波， 金晓丽， 邬飞波， 张国平*
(浙江大学农业与生物技术学院，浙江省作物种质资源重点实验室，杭州 310058)
Genotypic variations of N use efficiency in Tibetan wild and cultivated barleys
Yang Li’na, Hu Hongliang, Zhu Bo, Jin Xiaoli, Wu Feibo, Zhang Guoping*
(Key Laboratory of Crop Germplasm Resource of Zhejiang Province, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China)
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摘要： 以82份西藏野生大麦和16份栽培大麦为材料，利用水培试验，以生物量和氮素吸收为指标，比较了它们耐低氮的基因型差异.结果表明低氮耐性的基因型差异显著，野生大麦的变异大于栽培大麦.在此基础上，以鉴定获得的2个耐低氮野生大麦（XZ113和XZ149），1个中度耐低氮野生大麦（XZ125）和1个耐低氮栽培大麦品种（ZD9）为材料，研究它们在不同氮浓度下的生长与生理表现.氮素吸收动力学分析表明，ZD9与XZ113的最大吸收速率(Vmax)相似，但ZD9的亲和系数（Km）值较小，与XZ149相近；在供试的4个基因型中，XZ113的光合能力较强，而ZD9的硝酸还原酶活性较高.表明西藏一年生野生大麦的耐低氮机制可能与栽培大麦不同，为大麦低氮耐性或高效利用研究提供了新的种质材料.
Abstract: Tibetan annual wild barley is an ancestor of cultivated barley and rich in genetic diversity. Moreover, some wild barley accessions show high tolerance to poor soil. In this research, 82 wild barley accessions and 16 cultivated barley genotypes were firstly used in a hydroponic experiment, in order to compare their tolerance to low nitrogen (N). The results showed that there was a significant difference among genotypes in their low N tolerance, with the wild barley having wider variation than the cultivated barley. On the basis of the results obtained in the first experiment, two low-N tolerant wild genotypes (XZ113 and XZ149), one moderately tolerant wild genotype (XZ125) and one cultivated barley genotype (ZD9) were selected and used in the second experiment, to determine their growth and physiological performance under different N levels. The analysis of N-uptake dynamics showed that ZD9 and XZ113 had similar maximum uptake velocity (Vmax), and ZD9 had the smaller MichaelisMenten constant (Km), similar to that of XZ149. Among the four barley genotypes, XZ113 had higher photosynthetic rate and ZD9 had higher nitrate reductase activity. The current results indicate that Tibetan wild barley has different mechanisms underlying low N tolerance from those in cultivated barley, and may provide the elite germplasm with low N tolerance or high N use efficiency for modern barley breeding.
出版日期: 2014-03-20
CLC:  S 512.3  
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朱波
金晓丽
邬飞波
张国平*
杨丽娜
胡宏亮

引用本文:

杨丽娜， 胡宏亮， 朱波， 金晓丽， 邬飞波， 张国平*. 西藏野生大麦与栽培大麦氮利用效率的基因型差异(英文)[J]. 浙江大学学报(农业与生命科学版), 2014, 40(2): 155-164.

Yang Li’na, Hu Hongliang, Zhu Bo, Jin Xiaoli, Wu Feibo, Zhang Guoping*. Genotypic variations of N use efficiency in Tibetan wild and cultivated barleys. Journal of Zhejiang University (Agriculture and Life Sciences), 2014, 40(2): 155-164.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2013.09.021    或    http://www.zjujournals.com/agr/CN/Y2014/V40/I2/155

[1] 朱波， 曾建斌， 吴德志， 蔡圣冠， 杨丽娜， 张国平*. 青藏高原一年生野生大麦耐低钾种质的鉴定及其生理特性(英文)[J]. 浙江大学学报(农业与生命科学版), 2014, 40(2): 165-174.